| Budget Amount *help |
¥17,940,000 (Direct Cost: ¥13,800,000、Indirect Cost: ¥4,140,000)
Fiscal Year 2013: ¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000)
Fiscal Year 2012: ¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000)
Fiscal Year 2011: ¥9,490,000 (Direct Cost: ¥7,300,000、Indirect Cost: ¥2,190,000)
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| Research Abstract |
We tried to vitrify zebrafish oocytes at middle stage III and stage I successfully as a model of fish oocytes and ovaries, respectively. Oocytes at both stages were injected with cRNAs of water/cryoprotectant channels and anti-freeze proteins for exogenous expression of these proteins, and vitrified with a propylene glycol-based solution. Only oocytes expressing both proteins survived vitrification just after warming. However, the oocytes were dead after 1 h of culture. Further improvement is needed to succeed in the cryopreservation of fish oocytes and ovaries.
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