Development of the cryopreservation method for fish oocytes and ovaries
Project/Area Number |
23300158
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Research Category |
Grant-in-Aid for Scientific Research (B)
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Allocation Type | Single-year Grants |
Section | 一般 |
Research Field |
Laboratory animal science
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Research Institution | Kochi University |
Principal Investigator |
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Co-Investigator(Kenkyū-buntansha) |
EDASHIGE Keisuke 高知大学, 教育研究部総合科学系, 教授 (30175228)
KOSHIMOTO Chihiro 宮崎大学, フロンティア科学実験総合センター, 教授 (70295210)
MATSUKAWA Kazutsugu 高知大学, 教育研究部総合科学系, 准教授 (00532160)
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Project Period (FY) |
2011-04-01 – 2014-03-31
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Project Status |
Completed (Fiscal Year 2013)
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Budget Amount *help |
¥17,940,000 (Direct Cost: ¥13,800,000、Indirect Cost: ¥4,140,000)
Fiscal Year 2013: ¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000)
Fiscal Year 2012: ¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000)
Fiscal Year 2011: ¥9,490,000 (Direct Cost: ¥7,300,000、Indirect Cost: ¥2,190,000)
|
Keywords | 凍結保存 / 魚類卵子 / アクアポリン / 不凍タンパク質 / 水チャンネル / 不凍タンパク |
Research Abstract |
We tried to vitrify zebrafish oocytes at middle stage III and stage I successfully as a model of fish oocytes and ovaries, respectively. Oocytes at both stages were injected with cRNAs of water/cryoprotectant channels and anti-freeze proteins for exogenous expression of these proteins, and vitrified with a propylene glycol-based solution. Only oocytes expressing both proteins survived vitrification just after warming. However, the oocytes were dead after 1 h of culture. Further improvement is needed to succeed in the cryopreservation of fish oocytes and ovaries.
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Report
(4 results)
Research Products
(73 results)
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[Journal Article] Increased metabolite levels of glycolysis and pentose phosphate pathway in rabbit atherosclerotic arteries and hypoxic macrophage2014
Author(s)
Atsushi Yamashita, Yan Zhao, Yunosuke Matsuura, Kazuaki Yamasaki, Sayaka Moriguchi-Goto, Chihiro Sugita, Takashi Iwakiri, Nozomi Okuyama, Chihiro Koshimoto, Keiichi Kawai, Nagara Tamaki, Songji Zhao, Yuji Kuge, Yujiro Asada
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Journal Title
PLos One
Volume: 9
Issue: 1
Pages: e86426-e86426
DOI
Related Report
Peer Reviewed / Open Access
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[Journal Article] Effects of Cholesterol-Loaded Cyclodextrins on the Rate and the Quality of Motility in Frozen and Thawed Rabbit Sperm2014
Author(s)
Kazutoshi Nishijima, Shinji Yamaguchi, Mai Tanaka, Yusuke Sakai, Chihiro Koshimoto, Masatoshi Morimoto, TeruoWatanabe, Jianglin Fan, Shuji Kitajima
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Journal Title
Experimental Animals
Volume: 63
Issue: 2
Pages: 149-154
DOI
NAID
ISSN
0007-5124, 1341-1357, 1881-7122
Related Report
Peer Reviewed / Open Access
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[Journal Article] Rapid movement of water and cryoprotectants in pig expanded blastocysts via channel processes : its relevance to their higher tolerance to cryopreservation2013
Author(s)
Bo Jin, Ryu-ichi Higashiyama, Yu-ichi Nakata, Jun-ichi Yonezawa, Shangdan Xu, Masashi Miyake, Sei-ichi Takahashi, Kazuhiro Kikuch, Ken-ichi Yazawa, Shuhei Mizobuchi, Saori Niimi, Mizuho Kitayama, Chihiro Koshimoto, Kazutsugu Matsukawa, Magosaburo Kasai, Keisuke Edashige
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Journal Title
Biology of Reproduction
Volume: 89
Issue: 4
Pages: 1-12
DOI
Related Report
Peer Reviewed
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[Journal Article] Arterial <sup>18</sup>F-Fluorodeoxyglucose Uptake Reflects Balloon Catheter-Induced Thrombus Formation and Tissue Factor Expression via Nuclear Factor-κB in Rabbit Atherosclerotic Lesions2013
Author(s)
Atsushi Yamashita, Yan Zhao, Songji Zhao, Yunosuke Matsuura), Chihiro Sugita, Takashi Iwakiri, Nozomi Okuyama, Kazuyo Ohe, Chihiro Koshimoto, Keiichi Kawai, Nagara Tamaki, Yuji Kuge, Yujiro Asada.
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Journal Title
Circulation Journal
Volume: 77
Issue: 10
Pages: 2626-2635
DOI
NAID
ISSN
1346-9843, 1347-4820
Related Report
Peer Reviewed
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[Presentation] ^<18>F-FDG uptake reflects thrombus formation and tissue factor expression via nuclear factor-κB in rabbit atherosclerotic lesions. American Heart Association2012
Author(s)
Atsushi Yamashita, Yan Zhao, Songji Zhao, Yunosuke Matsuura, Chihiro Sugita, Takashi Iwakiri, Kazuyo Ohe, Chihiro Koshimoto, Keiichi Kawai, Nagara Tamaki, Yuji Kuge, Yujiro Asada
Organizer
Scientific Session
Place of Presentation
JW Marriott Los Angeles at LA Live, Los Angeles, California
Related Report
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[Presentation] The role of aquaporin 9 in the movement of DMSO and acetamide in mouse morulae2012
Author(s)
Keisuke Edashige, Shinsuke Seki, Hayato Arimura, Kan Matsuo, Yu-ichi Nakata, Bo Jin, Kazutsugu Matsukawa, Chihiro Koshimoto, Magosaburo Kasai
Organizer
SSR's 45th Annual Meeting of Society of the Study of Reprocution
Place of Presentation
The Alumni Hall at the Hetzel Union Building, The Pennsylvania State University, University Park, Pennsylvania
Related Report
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[Presentation] The role of aquaporin 9 in the movement of DMSO and acetamide in mouse morulae.2012
Author(s)
Keisuke Edashige, Shinsuke Seki, Hayato Arimura, Kan Matsuo, Yu-ichi Nakata, Bo Jin, Kazutsugu Matsukawa, Chihiro Koshimoto, Magosaburo Kasai.
Organizer
45th Annual Meeting of Society for the Study of Reprocution
Place of Presentation
The Alumni Hall at the Hetzel Union Building, The Pennsylvania State University, (Pennsylvania state, USA)
Related Report
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[Presentation] 18F-FDG uptake reflects thrombus formation and tissue factor expression via nuclear factor-κB in rabbit atherosclerotic lesions.2012
Author(s)
A Yamashita, Y Zhao, S. Zhao, Y Matsuura, C Sugita, T Iwakiri, K Ohe, C Koshimoto, K Kawai, N Tamaki, Y Kuge, Y Asada.
Organizer
American Heart Association, Scientific Session 2012.
Place of Presentation
JW Marriott Los Angeles at LA Live (California, USA)
Related Report
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[Presentation] オキナワトゲネズミTokudaia muenninkiの2009年調査以降に確認された新たな生息地2011
Author(s)
山田文雄, 中田勝士, 村田知慧, 木戸文香, 黒岩麻里, 城ヶ原貴通, 越本知大, 三谷匡, 阿部愼太郎, 河内紀浩, 小高信彦, 高嶋敦史, 安村茂樹
Organizer
第48回沖縄生物学会大会・第47回日本動物分類学会大会合同大会
Place of Presentation
琉球大学西原キャンパス,沖縄県中頭郡西原町
Related Report
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[Presentation] 沖縄島北部におけるオキナワトゲネズミTokudaia muenninkiの2009年調査以降に確認された新たな生息地2011
Author(s)
山田文雄, 中田勝士, 村田知慧, 木戸文香, 黒岩麻里, 城ヶ原貴通, 越本知大, 三谷匡, 阿部慎太郎, 河内紀浩, 小高信彦, 高嶋敦史
Organizer
日本哺乳類学会2011年度大会
Place of Presentation
宮崎市民プラザ&宮崎観光ホテル,宮崎市
Related Report
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