role of mechanosensitive response of organelle in dynamic biomechanics of cardiomyocytes

• Project/Area Number: 23300167
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Biomedical engineering/Biological material science
• Research Institution: Okayama University
• Principal Investigator: IRIBE Gentaro 岡山大学, 医歯(薬)学総合研究科, 講師 (90284885)
• Co-Investigator(Kenkyū-buntansha): WAKIMOTO Shuichi 岡山大学, 大学院・自然科学研究科, 准教授 (40452560)
• Co-Investigator(Renkei-kenkyūsha): NARUSE Keiji 岡山大学, 大学院・医歯薬学総合研究科, 教授 (40252233)
• Project Period (FY): 2011-04-01 – 2014-03-31
• Project Status: Completed (Fiscal Year 2013)
• Budget Amount: ¥18,720,000 (Direct Cost: ¥14,400,000、Indirect Cost: ¥4,320,000); Fiscal Year 2013: ¥3,770,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥870,000); Fiscal Year 2012: ¥3,770,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥870,000); Fiscal Year 2011: ¥11,180,000 (Direct Cost: ¥8,600,000、Indirect Cost: ¥2,580,000)
• Keywords: 機械感受性 / ミトコンドリア膜電位 / 活性酸素 / カルシウムスパーク / 心筋細胞 / 機械刺激 / ミトコンドリア / 機械電気帰還現象

Research Abstract

Calcium spark is diastolic spontaneous calcium releasing event from ryanodine receptors (RyR) on sarcoplasmic reticulum. We have previously reported that myocardial stretch increases calcium spark rate. It has been regarded that stretch-induced increase in reactive oxygen species (ROS) production from NADPH oxidase stimulates RyR to cause the phenomenon. However, the present study revealed that stretch-induced increase in ROS production is derived from mitochondria.

Research Products

• [Journal Article] Effect of azelnidipine and amlodipine on single cell mechanics in mouse cardiomyocytes. (2013)
  • Author(s): Iribe G, Kaihara K, Ito H, Naruse K.
  • Journal Title: Eur J Pharmacol Volume: 715 Issue: 1-3 Pages: 142-146
  • DOI: 10.1016/j.ejphar.2013.05.030
  • Peer Reviewed
• [Journal Article] Role of Sarcolemmal BKCa Channels in Stretch-Induced Extrasystoles in Isolated Chick Hearts (2011)
  • Author(s): Iribe G, Jin H, Naruse K
  • Journal Title: Circulation Journal Volume: 75 Issue: 11 Pages: 2552-2558
  • DOI: 10.1253/circj.CJ-11-0486
  • NAID: 10030031956
  • ISSN: 1346-9843, 1347-4820
  • Peer Reviewed
• [Journal Article] Minimum information about a cardiac electrophysiology experiment (MICEE) : Standardised reporting for model reproducibility, interoperability, and data sharing (2011)
  • Author(s): Quinn, T.A.
  • Journal Title: Progress in Biophysics & Molecular Biology Volume: 107 Issue: 1 Pages: 4-10
  • DOI: 10.1016/j.pbiomolbio.2011.07.001
  • Peer Reviewed
• [Presentation] Involvement of TRPC in the slow force response observed in mouse ventricular myocytes (2014)
  • Author(s): Yamaguchi Y, Kaneko T, Naruse K, Iribe G
  • Organizer: Biophysical Society 58th Annual Meeting
  • Place of Presentation: San Francisco, USA
• [Presentation] Mechano-electric interaction and arrhythmia (2014)
  • Author(s): Iribe G
  • Organizer: KPS-PSJ Symposium, the 91st Annual Meeting of the Physiological Society of Japan
  • Place of Presentation: Kagoshima, Japan
  • Invited
• [Presentation] Mitochondrial reactive oxygen species (ROS) production causes stretch-induced increase in calcium spark rate (2013)
  • Author(s): Iribe G, Kaihara K, Naruse K
  • Organizer: Venice Arrhythmia 2013
  • Place of Presentation: Venice, Italy
• [Presentation] Improvement in carbon fiber technique for cardiomyocyte mechanics and mechano-electric coupling study (2013)
  • Author(s): Iribe G, Kaneko T, Yamaguchi Y, Kaihara K, Naruse K
  • Organizer: 35th Annual International Conference of the IEEE Engineering in Medicine and Biology Society
  • Place of Presentation: Osaka, Japan
• [Presentation] Non-sarcolemmal stretch-activated channels (2013)
  • Author(s): Iribe G
  • Organizer: 6th Internationla Workshop, Cardiac Mechano-Electric Coupling and Arrhythmias
  • Place of Presentation: Oxford, UK
  • Invited
• [Presentation] Non-sarcolemmal stretch-activated channels (2013)
  • Author(s): Iribe G
  • Organizer: 6th International Workshop, Cardiac Mechano-Electric Coupling and Arrhythmias
  • Place of Presentation: Oxford, UK
  • Invited
• [Presentation] Mitochondrial reactive oxygen species (ROS) production causes stretch-induced increase in calcium spark rate (2013)
  • Author(s): Iribe G, Kaihara K, Naruse K
  • Organizer: Venice Arrhythmia
  • Place of Presentation: Venice, Italy
• [Presentation] Possible role of mitochondria on stretch-induced increase in calcium spark rate (2013)
  • Author(s): 入部玄太郎
  • Organizer: Gordon Research Conference: Cardiac Arrhythmia Mechanisms
  • Place of Presentation: Ventura, USA
• [Presentation] Improvement in carbon fiber technique for cardiomyocyte mechanics and mechano-electric coupling study (2013)
  • Author(s): 入部玄太郎
  • Organizer: The 35th Annual International Conference of the IEEE Engineering in Medicine and Biology Society
  • Place of Presentation: 大阪
• [Presentation] A novel cardiomyocyte stretch system for single cell mechanics study under physiological loading condition (2012)
  • Author(s): 金子智之
  • Organizer: World Congress on Medical Physics and Biomedical Engineering and the 19th International Conference on Medical Physics
  • Place of Presentation: 北京，中国
• [Presentation] Development of advanced stretch system for single isolated cardiomyocyte (2012)
  • Author(s): 金子智之
  • Organizer: 第51回日本生体医工学会大会
  • Place of Presentation: 福岡
• [Presentation] Effects of axial stretch on calcium handling in sarcoplasmic reticulum and mitochondria in cardiac myocytes (2011)
  • Author(s): Iribe, G.
  • Organizer: 5th Shanghai International Conference on Biophysics and Molecular biology
  • Place of Presentation: 上海
  • Year and Date: 2011-11-06
• [Presentation] Role of SAKCa channels in stretch-induced extrasystoles (2011)
  • Author(s): Iribe, G.
  • Organizer: 8th International Congress of Comparative Physiology
  • Place of Presentation: 名古屋
  • Year and Date: 2011-06-03
• [Presentation] 機械受容チャネルと不整脈 (2011)
  • Author(s): 入部玄太郎
  • Organizer: 第50回日本生体医工学会大会
  • Place of Presentation: 東京
  • Year and Date: 2011-04-29
• [Book] Non-sarcolemmal stretch-activated channels. In: Kohl P, Sachs F, Franz MR, editors. Cardiac mechano-electric coupling and arrhythmias (2011)
  • Author(s): Iribe G, Kohl P
  • Publisher: Oxford: Oxford University
• [Book] Non-sarcolemmal stretch-activated channels in Cardiac mechano-electric coupling and arrhythmias (2011)
  • Author(s): Iribe, G.
  • Total Pages: 7
  • Publisher: Oxford University Press
• [Book] アクチュエータが切り拓く医療、福祉(医学、バイオ研究での利用)アクチュエータが未来を創る (2011)
  • Author(s): 入部玄太郎
  • Total Pages: 10
  • Publisher: 産業図書