| Project/Area Number |
23300172
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Biomedical engineering/Biological material science
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| Research Institution | Hiroshima International University |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
NAKAMURA Kazufumi 岡山大学, 岡山大学病院, 准教授 (10335630)
KATAOKA Noriyuki 川崎医療福祉大学, 医療技術学部, 教授 (20250681)
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| Project Period (FY) |
2011-04-01 – 2014-03-31
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| Project Status |
Completed (Fiscal Year 2013)
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| Budget Amount *help |
¥18,200,000 (Direct Cost: ¥14,000,000、Indirect Cost: ¥4,200,000)
Fiscal Year 2013: ¥3,510,000 (Direct Cost: ¥2,700,000、Indirect Cost: ¥810,000)
Fiscal Year 2012: ¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000)
Fiscal Year 2011: ¥10,270,000 (Direct Cost: ¥7,900,000、Indirect Cost: ¥2,370,000)
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| Keywords | 心筋線維 / 心臓 / 発達 / X線回折 / 心筋細胞内カルシウム濃度 / 左心室圧 / 左心室容積 / 左心室圧容積関係 / PassiveStiffness / 心筋発達 / ラット / 筋線維 / 筋節構造 |
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| Research Abstract |
Isolated adult cardiomyocytes show rod shape with clear transverse striation pattern. However, isolated neonatal rat cardiomyocytes or induced cardiomyocytes of embryonic stem cells show star shape without clear transverse striation pattern. To analyze the development of structure of myofilament of cardiomyocytes, we performed x-ray diffraction analysis using Spring-8. We found that myofilament of neonatal rat heart had poor aligned myofilament lattice. the averaged inter filament lattice spacing was not so different during development over two weeks, deviation of interfilament lattice spacing was quite large at birth and decreased with development. We also considered how the poorly developed myofilament creates enough pressure to perfuse whole body. According to Laplace's law, small ventriclele requires small tension to create the enough pressure. Then, the well aligned myofilament can provide enough pressure even in the well developed ventricle.
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