| Budget Amount *help |
¥13,650,000 (Direct Cost: ¥10,500,000、Indirect Cost: ¥3,150,000)
Fiscal Year 2014: ¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000)
Fiscal Year 2013: ¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000)
Fiscal Year 2012: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
Fiscal Year 2011: ¥6,630,000 (Direct Cost: ¥5,100,000、Indirect Cost: ¥1,530,000)
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| Outline of Final Research Achievements |
Development of chiral specific sensor is needed for analysis of the role of D-amino acids in central nervous system, however chiral specificity of an electrode grafted with molecularly imprinted polymer (MIP) is declined in aqueous system. During the supported term, chiral specific sensing with MIP of an amino acid can be performed by blending hydrophilic and hydrophobic crosslinking monomers.
Voltage sensitive dye (VSD) imaging technique is needed to analyze dependency of neural network on D-amino acids. However VSD imaging is yet to be established for Aplysia central nervous system. We developed new VSD imaging method by using di-4-ANEPPS and tetraethyl ammonium cation. The method successfully detect action potential responding to taste stimulation on the mouth of Aplysia.
Those established method is feasible for analysis of the role of D-amino acids in central nervous system which is suspected to be important factor of nervous deceases.
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