| Budget Amount *help |
¥18,590,000 (Direct Cost: ¥14,300,000、Indirect Cost: ¥4,290,000)
Fiscal Year 2013: ¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000)
Fiscal Year 2012: ¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2011: ¥10,400,000 (Direct Cost: ¥8,000,000、Indirect Cost: ¥2,400,000)
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| Research Abstract |
The purpose of this study was to develop a super-selective molecular delivery system of lymph node metastasis via lymphatic vessels using nanobubbles (NBs) and ultrasound (US). We used MXH10/Mo-lpr/lpr mice exhibiting remarkable systemic lymphadenopathy, with proper axillary lymph nodes (PALNs) and subiliac lymph nodes (SiLNs) that are 6 to 12 mm in diameter. First, we identified 22 different LNs situated in the head and neck, limbs, thoracic and abdominal regions. The metastasis route was found to be via the efferent lymphatic vessels of the SiLN, and metastasis incidence depended on the number of cells injected, the injection duration and the SiLN volume. Next, we investigated drug delivery efficiency to the lymph node metastasis by the molecular delivery method using NBs and US. It was found that exposure to US in the presence of NBs enhanced the delivery of fluorophores into the lymphocytes near the lymphatic channels, compared to the use of US in the absence of NBs.
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