Development of cancer therapy inducing histone acethylation at the specific region of cancer genome

• Project/Area Number: 23300344
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Carcinogenesis
• Research Institution: Chiba Cancer Center (Research Institute)
• Principal Investigator: NAGASE Hiroki 千葉県がんセンター(研究所), がん遺伝創薬研究室, 研究所長 (90322073)
• Co-Investigator(Kenkyū-buntansha): 渡部 隆義 千葉県がんセンター(研究所), がん遺伝創薬研究室, 研究員 (60526060)
• Co-Investigator(Renkei-kenkyūsha): 板東 俊和 (20345284) ; 杉山 弘 (50183843)
• Project Period (FY): 2011-04-01 – 2014-03-31
• Project Status: Completed (Fiscal Year 2013)
• Budget Amount: ¥22,490,000 (Direct Cost: ¥17,300,000、Indirect Cost: ¥5,190,000); Fiscal Year 2013: ¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000); Fiscal Year 2012: ¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000); Fiscal Year 2011: ¥13,910,000 (Direct Cost: ¥10,700,000、Indirect Cost: ¥3,210,000)
• Keywords: ピロールイミダゾールポリアミド / ヒストンアセチル化 / ヒストン脱アセチル化酵素阻害剤 / がん / エピジェネティクス / ヒストン修飾 / エピジェネティク / 癌 / リプログラミング / iPS細胞 / SAHA / 山中因子 / 内在性遺伝子の再発現

Research Abstract

Tumor suppressor genes have anti-tumor effect leading antiproliferative and antiapoptotic effects. Tumor also repress expression of tumor specific antigen to escape immunological reaction against cancer. We are generating drugs of DNA sequence-specific Pyrrole-Imidazole polyamide-SAHA conjugates which reexpress repressed tumor suppressor gene endogenously in cancers. SAHA is an HDACi and activate gene expression. PIP can deliver SAHA to the specific gene. This PIP-SAHA may be a promising chemicals to cure cancer patients, effective drug has not yet been developed though. However in the course of this study we have successfully collaborated with Kyoto University and developed the drug which has generated iPS cells in mice.

Research Products

• [Journal Article] Distinct DNA-based epigenetic switches trigger differential transcriptional activation in human dermal fibroblasts (2014)
  • Author(s): Pandian GN, Nagase H
  • Journal Title: Scientific Reports Volume: 4 Issue: 1 Pages: 3843-3843
  • DOI: 10.1038/srep03843
  • Peer Reviewed
• [Journal Article] Inhibition of malignant phenotypes of human osteosarcoma cells by a gene silencer, a pyrrole-imidazole polyamide, which targets E-box (2014)
  • Author(s): Taniguchi M, Nakai Y, Ozaki T, Koshikawa N, Kojima T, Kataba M, Oguni A, Matsuda H, Yoshida Y, Tokuhashi Y, Fukuda N, Ueno T, Soma T, Nagase H
  • Journal Title: FEBS Open Bio Volume: (in press)
• [Journal Article] Nr4a3, a possibile oncogenic factor for neuroblastoma associated with CpGi methylation within the third exon. (2014)
  • Author(s): Uekusa S, Kawashima H, Sugito K, Yoshizawa S, Shinojima Y, Igarashi J, Ghosh S, Wang X, Fjiwara K, Ikeda T, Koshinaga T, Soma M and Nagase H.
  • Journal Title: International Journal of Oncology Volume: 44 Issue: 5 Pages: 1669-1677
  • DOI: 10.3892/ijo.2014.2340
  • Peer Reviewed / Open Access
• [Journal Article] Inhibition of malignant phenotypes of human osteosarcoma cells by a gene silencer, a pyrrole-imidazole polyamide, which targets E-box. (2014)
  • Author(s): 134. Taniguchi M, Nakai Y, Ozaki T, Koshikawa N, Kojima T, Kataba M, Oguni A, Matsuda H, Yoshida Y, Tokuhashi Y, Fukuda N, Ueno T, Soma T, Nagase H.
  • Journal Title: FEBS Open Bio Volume: 4 Issue: 1 Pages: 328-334
  • DOI: 10.1016/j.fob.2014.03.004
  • Peer Reviewed / Open Access
• [Journal Article] Obinata D Inhibition of MMP-9 by using a pyrrole-imidazole polyamide reduced cell invasion in renal cell carcinoma (2013)
  • Author(s): Sato A, Nagase H
  • Journal Title: International Journal of Oncology Volume: 43 Pages: 1441-6
• [Journal Article] Genome-wide screening of aberrant DNA methylation which associated with gene expression in mouse skin cancers (2013)
  • Author(s): Fujiwara K, Ghosh S, Liang P, Morien E, Soma M, Nagase H
  • Journal Title: Molecular Carcinogenesis Volume: e-pub Issue: 3 Pages: 1-11
  • DOI: 10.1002/mc.22085
  • Peer Reviewed
• [Journal Article] RNA binding properties of novel gene silencing pyrrole-imidazole polyamides (2013)
  • Author(s): Iguchi A, Fukuda N, Takahashi T, Watanabe T, Matsuda H, Nagase H, Bando T, Sugiyama H, Shimizu K
  • Journal Title: Biol Pharm Bull Volume: 36(7) Pages: 1152-8
  • NAID: 130003361484
• [Journal Article] A Synthetic Small Molecule Enforces T argeted Transcriptional Activation of Germ Cell Genes in a Human Somatic Cell (2013)
  • Author(s): Han L, Pandian GN, Junetha S, Sato S, Anandhakumar C, Taniguchi J, Saha A, Bando T, Nagase H and Sugiyama H
  • Journal Title: Angewandte Chemie Volume: 52(50) Pages: 13410-3
• [Journal Article] Sequence-Specific DNA Alkylation Targeting for Kras Codon 13 Mutation by Pyrrole-Imidazole Polyamide seco-CBI Conjugates (2013)
  • Author(s): Taylor RD, Asamitsu S, Takenaka T, Yamamoto M, Hashiya K, Kawamoto Y, Bando T, Nagase H, Sugiyama H
  • Journal Title: Chemistry Volume: 20 Issue: 5 Pages: 1310-1317
  • DOI: 10.1002/chem.201303295
  • Peer Reviewed
• [Journal Article] Identification of novel genomic regions with aberrant cytosine methylation in hematological malignancies (2013)
  • Author(s): Kobayashi Y, Fujiwara K, Hatta Y, Takeuchi J, Shinojima Y, Kawashima H, Igarashi J, Soma M, Nagase H.
  • Journal Title: Annals of Cancer Research and Therapy Volume: 21 Issue: 1 Pages: 01-13
  • DOI: 10.4993/acrt.21.01
  • NAID: 130003366756
  • ISSN: 1344-6835, 1880-5469
  • Peer Reviewed
• [Journal Article] Inhibition of MMP-9 by using a pyrrole-imidazole polyamide reduced cell invasion in renal cell carcinoma. (2013)
  • Author(s): Sato A, Nagase H, Obinata D, Fujiwara K, Fukuda N, Soma M, Yamaguchi K, Kawata N, Takahashi S.
  • Journal Title: International Journal of Oncology Volume: 43 Issue: 5 Pages: 1441-1446
  • DOI: 10.3892/ijo.2013.2073
  • Peer Reviewed
• [Journal Article] RNA binding properties of novel gene silencing pyrrole-imidazole polyamides. (2013)
  • Author(s): Iguchi A, Fukuda N, Takahashi T, Watanabe T, Matsuda H, Nagase H, Bando T, Sugiyama H, Shimizu K.
  • Journal Title: Biol Pharm Bull. Volume: 36 Pages: 1152-1158
  • NAID: 130003361484
  • Peer Reviewed
• [Journal Article] A Synthetic Small Molecule Enforces Targeted Transcriptional Activation of Germ Cell Genes in a Human Somatic Cell. (2013)
  • Author(s): Han L, Pandian GN, Junetha S, Sato S, Anandhakumar C, Taniguchi J, Saha A, Bando T, Nagase H and Sugiyama H.
  • Journal Title: Angewandte Chemie Volume: 52 Issue: 50 Pages: 13410-13413
  • DOI: 10.1002/anie.201306766
  • Peer Reviewed
• [Journal Article] DNA hypomethylation at the ZNF206-exon 5 CpG island associated with neuronal differentiation in mice and development of neuroblastoma in humans (2012)
  • Author(s): Kawashima H, Sugito K, Yoshizawa S, Uekusa S, Furuya T, Ikeda T, Koshinaga T, Shinojima Y, Hasegawa R, Mishra R, Igarashi J, Kimura M, Wang X, Fujiwara K, Gosh S and Nagase H
  • Journal Title: International Journal of Oncology Volume: 40 Pages: 31-39
• [Journal Article] エピジェネティクス疾患の新治療 チャイルドヘルス (2012)
  • Author(s): 永瀬浩喜
  • Journal Title: 診断と治療社 Volume: Vol.15, No.3 Pages: 38-41
• [Journal Article] A specific mitochondrial DNA mutation in mice regulates diabetes and lymphoma development (2012)
  • Author(s): Hashizume O, Shimizu A, Yokota M, Sugiyama A, Nakad K, Miyoshi H, Itami M, Ohira M, Nagase H, Takenaga K, and Hayashi J-I
  • Journal Title: Proc Natl Acad Sci U S A Jun 26 Volume: 109(26) Pages: 10528-33
  • NAID: 120007137266
• [Journal Article] A synthetic small molecule for rapid induction of multiple pluripotency genes in mouse embryonic fibroblasts (2012)
  • Author(s): Pandian GN, Nakano Y, Sato S, Morinaga H, Bando T, Nagase H, Sugiyama H
  • Journal Title: Sci Rep Volume: 2:544 Issue: 1 Pages: 544-544
  • DOI: 10.1038/srep00544
  • Peer Reviewed
• [Journal Article] Specific mitochondrial DNA mutation in mice regulates diabetes and lymphoma development (2012)
  • Author(s): Hashizume O, Shimizu A, Yokota M, Sugiyama A, Nakada K, Miyoshi H, Itami M, Ohira M, Nagase H, Takenaga K, Hayashi J.
  • Journal Title: Proc Natl Acad Sci USA. Volume: 109(26) Issue: 26 Pages: 10528-33
  • DOI: 10.1073/pnas.1202367109
  • NAID: 120007137266
  • Peer Reviewed
• [Journal Article] miR-34a is downregulated in cis-diamminedichloroplatinum treated sinonasal squamous cell carcinoma patients with poor prognosis (2012)
  • Author(s): Ogawa T, Saiki Y, Shiga K, Chen N, Fukushige S, Sunamura M, Nagase H, Hashimoto S, Matsuura K, Saijo S, Kobayashi T, Horii A.
  • Journal Title: Cancer Sci Volume: 103 Issue: 9 Pages: 1737-1743
  • DOI: 10.1111/j.1349-7006.2012.02338.x
  • Peer Reviewed
• [Journal Article] Identification of aberrant methylation regions in neuroblastoma by screening of tissue-specific differentially methylated regions (2012)
  • Author(s): Sugito K
  • Journal Title: Pediatric Blood & Cancer Volume: 60 Issue: 3 Pages: 383
  • DOI: 10.1002/pbc.24282
  • URL: https://pure.teikyo.jp/en/publications/e42ab754-2059-461f-88b8-06bcaf3d1fd7
  • Peer Reviewed
• [Journal Article] S100A4, frequently overexpressed in various human cancers, accelerates cell motility in pancreatic cancercells (2012)
  • Author(s): Sekine H, Chen N, Sato K, Saiki Y, Yoshino Y, Umetsu Y, Jin G, Nagase H, Gu Z, Fukushige S, Sunamura M, Horii A.
  • Journal Title: Biochem Biophys Res Commun Volume: 429 Issue: 3-4 Pages: 214-219
  • DOI: 10.1016/j.bbrc.2012.10.048
  • Peer Reviewed
• [Journal Article] Quantitation of pyrrole-imidazole polyamide in rat plasma by high performance liquid chromatography coupled with UV detection. (2012)
  • Author(s): Kamei T
  • Journal Title: Journal of Biomedicine and Biotechnology Volume: Article ID 715928 Pages: 1-10
  • DOI: 10.1155/2012/715928
  • Peer Reviewed
• [Journal Article] Quantitation of pyrrole-imidazole polyamide in rat plasma by high performance liquid chromatography coupled with UV detection (2012)
  • Author(s): Kamei T, Aoyama T, Tanaka C, Nagashima T, Aoyama Y, Hayashi H, Nagase H, Ueno T, Fukuda N, Matsumoto Y
  • Journal Title: Journal of Biomedicine and Biotechnology Volume: (In press)
  • Peer Reviewed
• [Journal Article] エピジェネティクス疾患の新治療 (2012)
  • Author(s): 永瀬浩喜
  • Journal Title: チャイルドヘルス診断と治療社 Volume: 15 Pages: 38-41
• [Journal Article] Development of a Novel Gene Silencer Pyrrole-Imidazole Polyamide Targeting Human Connective Tissue Growth Factor (2011)
  • Author(s): Wan JX, Fukuda N, Ueno T, Watanabe T, Matsuda H, Saito K, Nagase H, Matsumoto Y, Matsumoto K
  • Journal Title: BIOLOGICAL & PHARMACEUTICAL BULLETIN 34 Volume: 10 Pages: 1572-1577
  • NAID: 130001086589
• [Journal Article] Development of a Novel Gene Silencer Pyrrole-Imidazole Polyamide Targeting Human Connective Tissue Growth Factor (2011)
  • Author(s): Wan JX, Fukuda N, Ueno T, Watanabe T, Matsuda H, Saito K, Nagase H, Matsumoto Y, Matsumoto K
  • Journal Title: Biological and Pharmaceutical Bulletin Volume: 34 Issue: 10 Pages: 1572-1577
  • DOI: 10.1248/bpb.34.1572
  • NAID: 130001086589
  • ISSN: 0918-6158, 1347-5215
  • Peer Reviewed
• [Journal Article] Synthetic small molecules for epigenetic activation of pluripotent genes in mouse embryonic fibroblasts
  • Author(s): Pandian GN, Shinohara K, Ohtsuki A, Nakano Y, Minoshima M, Bando T, Nagase H, Yamada Y, Watanabe A, Terada N, Sato S, Morinaga H and Sugiyama H
  • Journal Title: ChemBioChem Volume: 12(18) Pages: 2822-2
• [Journal Article] Nr4a3, a possibile oncogenic factor for neuroblastoma associated with CpGi methylation within the third exon
  • Author(s): Uekusa S, Kawashima H, Sugito K, Yoshizawa S, Shinojima Y, Igarashi J, Ghosh S, Wang X, Fjiwara K, Ikeda T, Koshinaga T, Soma M and Nagase H
  • Journal Title: International Journal of Oncology Volume: (in press)
• [Presentation] A novel alkylating pyrrole‐imidazole polyamide specifically targeting KRAS codon12 mutations preferentially suppresses the malignant phenotypes in colon cancer cells bearing G12D or G12V mutation (2014)
  • Author(s): Nagase H, Hiraoka K, Inoue T, Ozaki T, Watanabe T, Shinohara K, Koshokawa N
  • Organizer: An AACR Special Conference on RAS ONCOGENES : FROM BIOLOGY TO THERAPY
  • Place of Presentation: オーランド 米国
• [Presentation] A novel alkylating pyrrole‐imidazole polyamide specifically targeting KRAS codon12 mutations preferentially suppresses the malignant phenotypes in colon cancer cells bearing G12D or G12V mutation. (2014)
  • Author(s): Hiroki Nagase, Kiriko Hiraoka,Takahiro Inoue,Toshinori Ozaki,Takayoshi Watanabe,Ken-ichi Shinohara,Nobuko Koshokawa
  • Organizer: An AACR Special Conference on RAS ONCOGENES:FROM BIOLOGY TO THERAPY
  • Place of Presentation: オーランド 米国
• [Presentation] KRASコドン12変異を標的とした分子標的アルキル化剤 (2014)
  • Author(s): 永瀬 浩喜、平岡桐子、井上隆博、渡部隆義
  • Organizer: 第18回日本がん分子標的治療学会学術集会
  • Place of Presentation: 仙台
• [Presentation] 転写因子特異的な TGFb, MMP9 抑制剤による線維化・炎症・がん転移の抑制 (2013)
  • Author(s): 永瀬浩喜
  • Organizer: 第9回免疫アジュバント研究会
  • Place of Presentation: さいたま市
  • Year and Date: 2013-09-06
• [Presentation] KRASコドン12変異を標的とした分子標的アルキル化剤 (2013)
  • Author(s): 永瀬 浩喜, 平岡桐子, 井上隆博, 渡部隆義
  • Organizer: 第18回日本がん分子標的治療学会学術集会
  • Place of Presentation: 仙台
  • Year and Date: 2013-06-26
• [Presentation] Automatic synthesis of efficient transcription inhibitors as anticancer agents designing sequence-specific DNA-banding molecules (2013)
  • Author(s): Nagase H, Koshikawa N, Watanabe T
  • Organizer: AACR American Association for Cancer Research AAUAL MEETING 2013
  • Place of Presentation: ワシントン DC 米国
• [Presentation] E-box 配列認識 PI ポリアミドによる抗腫瘍効果の検討 (2013)
  • Author(s): 藤原恭子, 谷口真史, 相馬正義, 永瀬浩喜
  • Organizer: 第22回日本癌病態治療研究会
  • Place of Presentation: 東京
• [Presentation] 変異型KRAS を標的とした塩基配列特異的アルキル化剤によるマウス移植ヒト大腸癌の増殖抑制 (2013)
  • Author(s): 井上貴博, 平岡桐子, 養田裕行, 杉本博一, 篠原憲一, 渡部隆義, 越川信子, 板東俊和, 杉山弘, 尾崎俊文, 永瀬浩喜
  • Organizer: 第36回日本分子生物学会年会
  • Place of Presentation: 神戸
• [Presentation] Genome-wide screening of aberrant DNA methylation which associated with gene expression in skin cancers (2013)
  • Author(s): 藤原恭子, 相馬正義, 永瀬浩喜
  • Organizer: 第36回日本分子生物学会年会
  • Place of Presentation: 神戸
• [Presentation] 変異型KRAS を標的とした 配列特異的アルキル化剤による腫瘍細胞の増殖抑制 (2013)
  • Author(s): 平岡桐子, 井上貴博, 養田裕行, 杉本博一, 篠原憲一, 渡部隆義, 越川信子, 板東俊和, 杉山弘, 尾崎俊文, 永瀬浩喜
  • Organizer: 第36回日本分子生物学会年会
  • Place of Presentation: 神戸
• [Presentation] DNA hypomethylation at the ZNF 206-exon 5 CpG island associatd with neuronal differentiation in mice and development of neuroblastoma in humans (2013)
  • Author(s): Kawashima H, Sugito K, Yoshizawa S, Uekusa S, Furuya T, Ikeda T, Koshinaga T , Shinojima Y, Hasegawa R, Mishra R, Igarashi J, Kimura M, Wang X, Fujiwara K, Gosh S, Nagase H
  • Organizer: 第55回日本小児血液・がん学会学術集会
  • Place of Presentation: 福岡
• [Presentation] 標的ゲノム配列特異的なヒストンアセチル化の誘導 (2013)
  • Author(s): 永瀬浩喜, 越川信子, 渡部隆義
  • Organizer: 第72回日本癌学会学術総会
  • Place of Presentation: 横浜
• [Presentation] 前立腺癌特異的融合遺伝子 TMPRSS2 -ERG の生成を抑制するピロール・イミダゾール(PI)ポリアミドの開発 (2013)
  • Author(s): 大日方大亮, 篠原恭子, 高山賢一, 浦野友彦, 永瀬浩喜, 相馬正義, 井上聡, 高橋悟
  • Organizer: 第72回日本癌学会学術総会
  • Place of Presentation: 横浜
• [Presentation] 肝癌進行を抑制する TGF-β1をターゲットにした PI ポリアミドの開発 (2013)
  • Author(s): 高木恵子, 高山忠利, 藤原恭子, 相馬正義, 永瀬浩喜
  • Organizer: 第72回日本癌学会学術総会
  • Place of Presentation: 横浜
• [Presentation] 抗腫瘍効果を持つ E-box 認識ピロール・イミダゾール・ポリアミドの開発 (2013)
  • Author(s): 藤原恭子, 相馬正義, 永瀬浩喜
  • Organizer: 第72回日本癌学会学術総会
  • Place of Presentation: 横浜
• [Presentation] 腎芽腫細胞株(G401)におけるKCNQ10T1遺伝子を標的としたPYRROLE‐IMIDAZOLE POL YAMIDE(PIP)の抗腫瘍効果の検討 (2013)
  • Author(s): 吉澤信輔, 杉藤公信, 星玲奈, 渡邉揚介,植草省太, 川島弘之, 大橋研介, 池田太郎,越永従道, 吉澤信輔, 星玲奈, 渡邉揚介, 藤原恭子, 永瀬浩喜
  • Organizer: 第55回日本小児血液・がん学会学術集会・第11回日本小児がん看護学会・第18回がんの子供を守る会公開シンポジウム
  • Place of Presentation: 福岡
• [Presentation] 新規 E ボックス認識化合物による MYC 依存性腫瘍の増殖抑制 (2013)
  • Author(s): 永瀬浩喜, 越川信子, 渡部隆義, 藤原恭子, Rajeev M
  • Organizer: 第55回日本小児血液・がん学会学術集会・第11回日本小児がん看護学会・第18回がんの子供を守る会公開シンポジウム
  • Place of Presentation: 福岡
• [Presentation] Automatic synthesis of efficient transcription inhibitors as anticancer agents designing sequence-specific DNA-banding molecules. (2013)
  • Author(s): Hiroki Nagase, Nobuko Koshikawa, Takayoshi Watanabe
  • Organizer: AACR American Association for Cancer Research AAUAL MEETING 2013
  • Place of Presentation: ワシントンDC アメリカ
• [Presentation] 転写因子特異的な TGFｂ、MMP9 抑制剤による線維化・炎症・がん転移の抑制 (2013)
  • Author(s): 永瀬浩喜
  • Organizer: 第9回免疫アジュバント研究会
  • Place of Presentation: さいたま
  • Invited
• [Presentation] 次世代半導体シークエンサーによるがん臨床ゲノム解析 (2013)
  • Author(s): ・ 永瀬浩喜, 横井左奈, 大平美紀, 影山肇, 中村友紀, 丸喜明, 伊丹真紀子, 片山稔
  • Organizer: 第51回日本癌治療学会学術集会
  • Place of Presentation: 京都
• [Presentation] 新規Eボックス認識化合物によるMYC依存性腫瘍の増殖抑制 (2013)
  • Author(s): 永瀬浩喜, 越川信子, 渡部隆義, 藤原恭子, MISHRA Rajeev
  • Organizer: 第55回日本小児血液・がん学会学術集会
  • Place of Presentation: 福岡
• [Presentation] 変異型KRASを標的とした塩基配列特異的アルキル化剤によるマウス移植ヒト大腸癌の増殖抑制 (2013)
  • Author(s): ・ 井上貴博, 平岡桐子, 養田裕行, 杉本博一, 篠原憲一, 渡部隆義, 越川信子, 尾崎俊文, 永瀬浩喜, 坂東俊和, 杉山弘
  • Organizer: 第36回日本分子生物学会年会
  • Place of Presentation: 神戸
• [Presentation] 変異型KRASを標的とした塩基配列特異的アルキル化剤による腫瘍細胞の増殖抑制 (2013)
  • Author(s): ・ 平岡桐子, 井上貴博, 養田裕行, 杉本博一, 篠原憲一, 渡部隆義, 越川信子, 尾崎俊文, 永瀬浩喜, 板東俊和, 杉山弘
  • Organizer: 第36回日本分子生物学会年会
  • Place of Presentation: 神戸
• [Presentation] DNA binding molecules : Chemical Genetic Switch to Regulate Cell Fate (2012)
  • Author(s): Nagase H
  • Organizer: The 8th Hebei Province Conference on Oncology
  • Place of Presentation: 滄州 China
  • Year and Date: 2012-08-25
• [Presentation] MYCN TENSCRIPTION SILENCER BY USING SYNTHETIC PYRROLE-IMIDAZOLE POL Y AMIDE MOLECULE IN NEUROBLASTOMA CELLS (2012)
  • Author(s): Watanabe T, Nagase H
  • Organizer: 7th INTERNA TIONAL SOCIETY OF PEDIA TRIC ONCOLOGY ASIA CONGRESS
  • Place of Presentation: Yogyokarta Indnesia
• [Presentation] MYCN TENSCRIPTION SILENCER BY USING SYNTHETIC PYRROLE-IMIDAZOLE POLYAMIDE MOLECULE IN NEUROBLASTOMA CELLS (2012)
  • Author(s): Takahiro Watanabe, Hiroki Nagase
  • Organizer: 7th INTERNATIONAL SOCIETY OF PEDIATRIC ONCOLOGY ASIA CONGRESS
  • Place of Presentation: Yogyokarta Indnesia
• [Presentation] DNA binding molecules：Chemical Genetic Switch to Regulate Cell Fate. (2012)
  • Author(s): Hiroki Nagase
  • Organizer: The 8th Hebei Province Conference on Oncology
  • Place of Presentation: 滄州 China.
• [Presentation] Target epigenetic regulation at a specific genomic region to induce stem cell like phenotype in neuroblastoma cells. (2012)
  • Author(s): Nagase H , Watanabe T, Koshikawa N, Bando T, Sugiyama H.
  • Organizer: 44th Congress of the International Society of Paediatric Oncology (SIOP)
  • Place of Presentation: London UK
• [Presentation] 哺乳類ゲノムの発生過程におけるＤＮＡメチル化のダイナミックな変化 (2012)
  • Author(s): Hiroki Nagase , Ping Liang , Fei Song , William A. Held
  • Organizer: 第６回エピジェネティクス研究会年会
  • Place of Presentation: 東京
• [Presentation] 小分子化合物によるMEFでの多能性細胞誘導遺伝子のエピジェネティックな活性化 (2012)
  • Author(s): 永瀬浩喜、渡部隆博、越川信子、井上貴博、平岡桐子、板東俊和、杉山弘
  • Organizer: 第２６回モロシヌス研究会
  • Place of Presentation: 東京
• [Presentation] ゲノム配列特異的ヒストン修飾変更:がん化メカニズムの探索と治療への応用 (2012)
  • Author(s): 永瀬 浩喜、 渡部 隆義、 越川 信子、 板東 俊和、 杉山 弘
  • Organizer: 第７１回日本癌学会学術総会
  • Place of Presentation: 札幌
• [Presentation] DNA結合合成化合物を用いたインビボでの標的遺伝子発現調節の試み (2012)
  • Author(s): 永瀬浩喜 越川信子
  • Organizer: 第16回 日本がん分子標的治療学会
  • Place of Presentation: 北九州市
• [Presentation] Molecular recognition of DNA : Application of Pyrrole-Imidazole (PI) polyamides for anti-inflammation and anti-cancer invasion (2011)
  • Author(s): Hiroki Nagase, Nobuko Koshikawa, Takahiro Watanabe
  • Organizer: The 16 the Japan-Korea Cancer Research Workshop
  • Place of Presentation: 札幌
  • Year and Date: 2011-12-12
• [Presentation] Molecular recognition of DNA : application of Pyrrole Imidazole polyamides for anti-inflammation and anti-cancer invasion (2011)
  • Author(s): Nagase H
  • Organizer: The 16th Japan-Korea Cancer Research Workshop
  • Year and Date: 2011-12-10
• [Presentation] "Genome sequence specific histone modification to regulate cell fate" 「ゲノム配列特異的なヒストン修飾阻害による細胞の運命変化」 (2011)
  • Author(s): Nagase H
  • Organizer: "Genome sequence specific histone modification to regulate cell fate" 「ゲノム配列特異的なヒストン修飾阻害による細胞の運命変化」
  • Place of Presentation: 名古屋国際会議場 第70回日本癌学会
  • Year and Date: 2011-10-04
• [Presentation] 合成 DNA 結合化合物を用いた多能細胞・疾患モデルに対する表現型の変更 (2011)
  • Author(s): 永瀬浩喜, 越川信子, 渡部隆義, 井上貴博, 石原優, 平岡桐子
  • Organizer: 第87回発生工学・疾患モデル研究会
  • Place of Presentation: 国立がんセンター
  • Year and Date: 2011-08-19
• [Presentation] 「E-box 結合転写因子の結合抑制によるMYC 下流遺伝子の制御 : がん抑制ピロールイミダゾール ポリアミド」 "Novel E-box binding PI polyamides inhibiting MYC-driven cell-proliferation" (2011)
  • Author(s): Nagase H
  • Organizer: 第15回日本分子標的学会
  • Place of Presentation: ホテル日航東京
  • Year and Date: 2011-06-24
• [Presentation] Cell permeable synthetic chemicals targeting a specific DNA sequence to modify the mammalian genome regulation (2011)
  • Author(s): Nagase H
  • Organizer: 1st China-Japan Symposium on Cancer Research
  • Place of Presentation: Shenzhen China
• [Presentation] DNA 結合化合物による疾患モデルマウスの表現型の変更 (2011)
  • Author(s): 永瀬浩喜, 渡部隆博, 越川信子, 井上貴博, 石原優, 平岡桐子, 藤原恭子
  • Organizer: 第25回モロシヌス研究会
  • Place of Presentation: 十日町新潟
• [Presentation] Cell permeable synthetic chemicals targeting a specific DNA sequence to modify the mammalian genome regulation (2011)
  • Author(s): Nagase H
  • Organizer: Japanese-German Cancer Workshop
  • Place of Presentation: Hiroshima, JAPAN
• [Presentation] Novel E-box Binding PI polyamides Inhibiting MYC Driven Cell Proliferation (2011)
  • Author(s): Nagase H
  • Organizer: International Society of Paediatric Oncology
  • Place of Presentation: Auckland, NewZealand
• [Presentation] Cell permeable synthetic chemicals targeting a specific DNA sequence to modify the mammalian genome regulation (2011)
  • Author(s): Hiroki Nagase
  • Organizer: China-Japan Symposium on Cancer Research
  • Place of Presentation: Shenzhen China
• [Presentation] E-box結合転写因子の結合抑制によるMYC下流遺伝子の制御:がん抑制ピロールイミダゾールポリアミドNovel E-box binding PI polyamides inhibiting MYC-driven cell-proliferation (2011)
  • Author(s): Hiroki Nagase
  • Organizer: 第15回日本分子標的学会
  • Place of Presentation: 東京
• [Presentation] Molecular Recognition of DNA : A tool for epigenetic DNA transcription regulation (2011)
  • Author(s): Hiroki Nagase, Takayoshi Watanabe, Ken-ichi Shinohara, Hiroshi Sugiyama
  • Organizer: The 3^<rd> EMBO meeting
  • Place of Presentation: Vienna, Austria
• [Presentation] ゲノム配列特異的なヒストン修飾阻害による細胞の運命変化 (2011)
  • Author(s): 永瀬浩喜、渡部隆義、杉山弘
  • Organizer: 第70回日本癌学会学術総会
  • Place of Presentation: 名古屋
• [Presentation] Target epigenetic regulation at a specific genomic region to induce stem cell like phenotype in nenroblastoma cells (2011)
  • Author(s): Hiroki Nagase, Takayoshi Watanabe, Nobuko Koshikawa, Toshikazu Bando, Hiroshi Sugiyama
  • Organizer: The 43^<rd> SIOP meeting第43回国際小児がん学会
  • Place of Presentation: Auckland, New Zealand
• [Book] 「遺伝子疾患モデルの作成と利用-がん」 第7章 皮膚および運動器系 第1節 表皮腫瘍誘発モデル (2012)
  • Author(s): 永瀬浩喜
• [Book] 「遺伝子疾患モデルの作成と利用―がん」 第7章 皮膚および運動器系 第１節 表皮腫瘍誘発モデル (2012)
  • Author(s): 永瀬浩喜
  • Total Pages: 633
  • Publisher: 株式会社 エル アイ シー
• [Remarks]
  • URL: http://www.pref.chiba.lg.jp/gan/kenkyujo/soshiki/iden/index.html
• [Remarks] 千葉県がんセンター研究所 がん遺伝創薬研究室
  • URL: http://www.pref.chiba.lg.jp/gan/kenkyujo/soshiki/iden/index.html
• [Remarks] がん研究分野の特性等を踏まえた支援活動
  • URL: http://ganshien.umin.jp/public/research/main/nagase/index.html
• [Remarks] 千葉県がんセンター研究所 がん遺伝創薬研究室
  • URL: http://www.chiba-cc.jp/inst/jp/organization/003.pdf
• [Remarks] がん研究分野の特性糖を踏まえた支援活動 研究紹介 永瀬浩喜
  • URL: http://ganshien.umin.jp/public/research/main/nagase/index.html
• [Remarks] 本課題の基礎となる特許「標的遺伝子特異的ヒストン修飾制御剤」を取得し、特許第4873510として平成23年12月2日に登録された。ただし、これは本研究を開始する前の成果により出願されたものである。
• [Remarks] 所属研究機関が作成したwebページ
  • URL: http://www.chiba-cc.jp/inst/jp/index.html
• [Patent(Industrial Property Rights)] ドライバーオンコジーンの遺伝子変異を標的にアルキル化する新規アルキル化剤 (2013)
  • Inventor(s): 永瀬浩喜, 杉山 弘, 板東俊和
  • Industrial Property Rights Holder: 千葉県および京都大学
  • Industrial Property Rights Type: 特許
  • Industrial Property Number: 2013-214044
  • Filing Date: 2013-10-11
• [Patent(Industrial Property Rights)] ドライバーオンコジーンの遺伝子変異を標的にアルキル化する新規アルキル化剤 (2013)
  • Inventor(s): 永瀬浩喜、杉山 弘、板東俊和
  • Industrial Property Rights Holder: 千葉県
  • Industrial Property Rights Type: 特許
  • Industrial Property Number: 2013-214044
  • Filing Date: 2013-10-11
• [Patent(Industrial Property Rights)] 標的遺伝子特異的ヒストン修飾制御剤 (2011)
  • Inventor(s): 永瀬浩喜, 杉山 弘, 鈴木 司, 板東俊和, 木村 真, 大朏彰道
  • Industrial Property Rights Holder: 日本大学
  • Industrial Property Rights Type: 特許
  • Acquisition Date: 2011-12-02