Disorder of cell adhesion system in tumor progression
| Project/Area Number | 23300351 |
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Tumor biology
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| Research Institution | Yokohama City University |
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Principal Investigator |
MIYAZAKI KAORU 横浜市立大学, 付置研究所, その他 (70112068)
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| Co-Investigator(Renkei-kenkyūsha) |
HIGASHI Shouichi 横浜市立大学, 大学院・生命ナノシステム科学研究科, 准教授 (10275076)
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| Project Period (FY) |
2011-04-01 – 2014-03-31
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| Project Status |
Completed(Fiscal Year 2013)
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| Budget Amount *help |
¥22,100,000 (Direct Cost : ¥17,000,000、Indirect Cost : ¥5,100,000)
Fiscal Year 2013 : ¥5,330,000 (Direct Cost : ¥4,100,000、Indirect Cost : ¥1,230,000)
Fiscal Year 2012 : ¥7,540,000 (Direct Cost : ¥5,800,000、Indirect Cost : ¥1,740,000)
Fiscal Year 2011 : ¥9,230,000 (Direct Cost : ¥7,100,000、Indirect Cost : ¥2,130,000)
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| Keywords | がん浸潤 / 細胞接着分子 / ラミニン332 / ラミニンγ2 / 上皮間葉変換（EMT） / CD44 / がん幹細胞 / 腫瘍血管 / 腫瘍-間質相互作用 / がん浸潤モデル / コラーゲンゲル / HGF / TGF-beta / ラミニン / 線維芽細胞 / EMT / TGF-β / 細胞接着 / 浮遊性増殖 / 浸潤モデル / 微小管 / コラーゲンマトリックス / がんの浸潤 / 浮遊増殖 / 腹水 |
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| Research Abstract |
Non-invasive tumor cells are fixed on basement membranes (BMs) by stably binding to cell adhesion proteins such as laminin-332 (Lm332). However, they start to invade the BMs and then stroma during tumor progression. In this study, we investigated the mechanism of tumor invasion, mainly focusing on the interaction of tumor cells with cell adhesion proteins. Major results are as follows.
Laminin gamma2 (Lm-g2), a subunit of Lm332, is often overexpressed in invasive human cancers and hence regarded as a tumor invasion marker. We found that (1) Lm-g2 is induced in association with epithelial-mesenchymal transition of tumor cells, and (2) amino-terminal fragments of Lm-g2 directly interacts with the cancer stem cell marker CD44 in metastatic cancer cells, leading to the stimulation of their migration. These results suggest the cooperation of the two important tumor markers for cancer progression. We also found that Lm-g2 acts on vascular endothelial cells, increasing vascular permeability.
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Report
(4results)
Research Products
(64results)
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[Journal Article] Amino-terminal fragments of laminin gamma2 chain retract vascular endothelial cells and increase vascular permeability.2014
Author(s)
Sato, H, Oyanagi, J., Komiya, E., Ogawa, T, Higashi, S., Miyazaki, K.
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Journal Title
Cancer Sci.
Volume : 105
Pages : 168-175
DOI
Related Report
Peer Reviewed / Open Access / Acknowledgement Compliant
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[Journal Article] Modulation of matrix metalloproteinase-9 secretion from tumor-associated macrophage-like cells by proteolytically processed laminin-332 (laminin-5).2014
Author(s)
Kamoshida, G., Ogawa, T., Oyanagi, J., Sato, H., Komiya, E., Higashi, S., Miyazaki, K., Tsuji, T.
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Journal Title
Clin. Exp. Metastasis
Volume : 31
Pages : 285-291
DOI
Related Report
Peer Reviewed
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[Journal Article] Angiomodulin (AGM/IGFBP-rP1) is a molecular marker of vascular endothelial cells activated by VEGF in human breast cancers.2014
Author(s)
Komiya, E, Sato, H., Ise, I., Watanabe, N., Higashi, H., Miyagi, Y., Miyazaki, K.
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Journal Title
Cancer Med
Volume : 印刷中
Issue : 3
Pages : 537-549
DOI
Related Report
Peer Reviewed / Open Access / Acknowledgement Compliant
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[Journal Article] Inhibition of transforming growth factor-β signaling potentiates tumor cell invasion into collagen matrix induced by fibroblast-derived hepatocyte growth factor.2014
Author(s)
Oyanagi, J., Kojima, N., Sato, H., Higashi, S., Kikuchi, K., Sakai, K., Matsumoto, K., Miyazaki, K.
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Journal Title
Exp Cell Res.
Volume : 印刷中
Pages : 267-279
DOI
Related Report
Peer Reviewed / Open Access / Acknowledgement Compliant
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