| Budget Amount *help |
¥19,760,000 (Direct Cost: ¥15,200,000、Indirect Cost: ¥4,560,000)
Fiscal Year 2013: ¥5,720,000 (Direct Cost: ¥4,400,000、Indirect Cost: ¥1,320,000)
Fiscal Year 2012: ¥5,720,000 (Direct Cost: ¥4,400,000、Indirect Cost: ¥1,320,000)
Fiscal Year 2011: ¥8,320,000 (Direct Cost: ¥6,400,000、Indirect Cost: ¥1,920,000)
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| Outline of Final Research Achievements |
An attempt of elucidation of molecular mechanism of the DOHaD in which fetal environmental factors are hypothesized to cause an epigenomic alteration and memory persisted in adulthood. Additionally we developed a new genome-wide methylation analysis (MSD-AFLP method). The Cyp1a1 promoter of the liver is hypomethylated from day 3 after birth when given TCDD to pregnant mice and the altered methylation level is maintained until after maturity. Because the Dnmt3b dissociation was found by ChIP assay, we assumed mechanistic possibility of passive demethylation. Whereas we tested whether this memory could happen in the adulthood. In the mouse liver of 10 weeks of age, it was found that hypomethylation was caused by the early point in time such as 24 hours after TCDD administration, suggesting that active demethylation should be considered.
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