High functionality of novel carbonyl sulfide hydrolase aiming at the effective removal of carbonyl sulfide

• Project/Area Number: 23310051
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Environmental technology/Environmental materials
• Research Institution: Tokyo University of Agriculture and Technology
• Principal Investigator: KATAYAMA Yoko 東京農工大学, (連合)農学研究科(研究院), 教授 (90165415)
• Co-Investigator(Kenkyū-buntansha): ODAKA Masahumi 東京農工大学, 大学院・工学研究院, 准教授 (20224248) ; NOGUCHI Keiichi 東京農工大学, 学内共同利用施設等, 准教授 (00251588)
• Project Period (FY): 2011-04-01 – 2014-03-31
• Project Status: Completed (Fiscal Year 2013)
• Budget Amount: ¥19,760,000 (Direct Cost: ¥15,200,000、Indirect Cost: ¥4,560,000); Fiscal Year 2013: ¥5,460,000 (Direct Cost: ¥4,200,000、Indirect Cost: ¥1,260,000); Fiscal Year 2012: ¥5,980,000 (Direct Cost: ¥4,600,000、Indirect Cost: ¥1,380,000); Fiscal Year 2011: ¥8,320,000 (Direct Cost: ¥6,400,000、Indirect Cost: ¥1,920,000)
• Keywords: 硫化カルボニル / 致死性有毒ガス / オゾン層破壊 / 酵素分解処理 / 分子育種 / 結晶構造解析 / 微生物分解 / 温室効果ガス / 分解微生物

Research Abstract

Carbonyl sulfide hydrolase (COSase) has been isolated from sulfur oxidizing bacterium. COSase catalyses degradation of carbonyl sulfide (COS) that is a trace constituent in the atmosphere and is considered as one of important gas affecting on the global climate especially on ozone depletion. The aim of our research is to obtain information to make highly efficient enzyme molecule so that powerful removal of COS will be possible. We introduced mutations of several amino acids around the sequence of the active center of the enzyme and alpha 5 helix that is a putative tunnel of substrate or reaction product by forming point mutation and examined the effects against COS degrading activity. Furthermore, we tried to isolate new COS degrading microorganisms from soil samples because soil environment is the major sink of atmospheric COS. Novel COS degrading microorganisms including bacteria and fungi were isolated from the soil.

Research Products

• [Journal Article] Carbonyl sulfide hydrolase from Thiobacillus thioparus strain THI115 is one of the β-carbonic anhydrase family enzymes (2013)
  • Author(s): Ogawa T, Noguchi K, Saito M, Nagahata Y, Kato H, Ohtaki A, Nakayama H, Dohmae N, Matsushita Y, Odaka M, Yohda M, Nyunoya H, Katayama Y
  • Journal Title: J. Am. Chem. Soc. Volume: 135 Issue: 10 Pages: 3818-3825
  • DOI: 10.1021/ja307735e
  • Peer Reviewed
• [Journal Article] Addressing the microbiological problems of cultural property and archive documents after earthquake and tsunami (2013)
  • Author(s): Gu JD, Kigawa R, Sato Y, Katayama Y
  • Journal Title: Int Biodeter Biodeg Volume: 85 Pages: 345-346
  • DOI: 10.1016/j.ibiod.2013.08.018
  • Peer Reviewed
• [Journal Article] Cloning and expression of a gene encoding novel thermostable thiocyanate- degrading enzyme from a mesophilic alpha- proteobacteria strain THI201 (2013)
  • Author(s): Hussain A, Ogawa T, Saito M, Sekine T, Nameki M, Matsushita Y, Hayashi T, Katayama Y
  • Journal Title: Microbiol Volume: 159 Issue: Pt_11 Pages: 2294-2302
  • DOI: 10.1099/mic.0.063339-0
  • Peer Reviewed
• [Journal Article] Bacterial Communities in Pigmented Biofilms Formed on the Sandstone Bas-Relief Walls of the Bayon Temple, Angkor Thom, Cambodia (2013)
  • Author(s): Kusumi A, Li X, Osuga Y, Kawashima A, Gu JD, Nasu M. Katayama Y
  • Journal Title: Microbes and Environments Volume: 28 Issue: 4 Pages: 422-431
  • DOI: 10.1264/jsme2.ME13033
  • NAID: 130003382014
  • ISSN: 1342-6311, 1347-4405
  • Peer Reviewed
• [Journal Article] Occurrence of Aspergillus allahabadii on sandstone at Bayon Temple, Angkor Thom, Cambodia (2013)
  • Author(s): Hu H, Ding S, Katayama Y. (他5名)
  • Journal Title: Int Biodeter Biodeg Volume: 76 Pages: 112-117
  • DOI: 10.1016/j.ibiod.2012.06.022
  • Peer Reviewed
• [Journal Article] Phylogenetic assessment of culture collection strains of Thiobacillus thioparus, and deWnitive 16S rRNA gene sequences for T.thioparus, T.denitriWcans, and Halothiobacillus neapolitanus (2012)
  • Author(s): Rich Boden, David Cleland, Peter N.Green, Yoko Katayama, Yoshihito Uchino, J.Colin Murrell, Donovan P.Kelly
  • Journal Title: Archives of Microbiology Volume: 194 Issue: 3 Pages: 187-195
  • DOI: 10.1007/s00203-011-0747-0
  • Peer Reviewed
• [Journal Article] Vertical distribution of carbonyl sulfide at Mt.Fuji, Japan (2012)
  • Author(s): Hiromi Kato, Yasuhito Igarashi, Tukiko Dokiya, Yoko Katayama
  • Journal Title: Water, Air, and Soil Pollution Volume: 223 Issue: 1 Pages: 159-167
  • DOI: 10.1007/s11270-011-0847-0
  • Peer Reviewed
• [Journal Article] Mycobacteria isolated from Angkor monument sandstones grow chemo-lithoautotrophically by oxidizing elemental sulfur (2011)
  • Author(s): Asako Kusumi, Xian Shu Li, Yoko Katayama
  • Journal Title: Frontiers in Microbiology Volume: 2 Pages: 104-109
  • DOI: 10.3389/fmicb.2011.00104
  • Peer Reviewed
• [Journal Article] Crystalline structure of polyethylene containing vinylene units in the main chain (2011)
  • Author(s): Naofumi Naga
  • Journal Title: Polymer Volume: 52 Issue: 21 Pages: 4857-4866
  • DOI: 10.1016/j.polymer.2011.08.058
  • Peer Reviewed
• [Journal Article] Rhodium-catalyzed olefin isomerization/enantioselective intramolecular Alder-Enereaction cascade (2011)
  • Author(s): Ryuichi Okamoto
  • Journal Title: Org.Lett. Volume: 13 Issue: 18 Pages: 4894-4897
  • DOI: 10.1021/ol201986e
  • Peer Reviewed
• [Journal Article] Properties and crystal structure of methylenetetrahydrofolate reductase from Thermus thermophilus HB8 (2011)
  • Author(s): 猪狩早雅(筆頭著者)、山田和弘(責任著者)(他6名)
  • Journal Title: PLoS One Volume: Vol.6 Issue: 8 Pages: e23716-e23716
  • DOI: 10.1371/journal.pone.0023716
  • Peer Reviewed
• [Journal Article] Linking the Conformational Flexibility and Kinetics : Catalytic 1,4-Type Friedel-Crafts Reactions of Phenols Utilizing 1,3-Diamine-Tethered-Guanidine/Bisthiourea Organocatalysts (2011)
  • Author(s): Yoshihiro Sohtome, Bongki Shin, Natsuko Horitsugi, Keiichi Noguchi, Kazuo Nagasawa
  • Journal Title: Chem.Asian J. Volume: 6 Issue: 9 Pages: 2463-2470
  • DOI: 10.1002/asia.201100363
  • Peer Reviewed
• [Journal Article] Osteoclast-forming suppressing compounds, gargalols A, B, and C, from the edible mushroom grifola gargal (2011)
  • Author(s): Jing Wu
  • Journal Title: Tetrahedron Volume: 67 Issue: 35 Pages: 6576-6581
  • DOI: 10.1016/j.tet.2011.05.091
  • Peer Reviewed
• [Journal Article] Rhodium- Catalyzed Highly Enantioselective Cyclizations of g-Alkynylaldehydes with Acyl Phosphonates: Ligand and Substituent-Controlled C-P or C-H Bond Cleavage (2011)
  • Author(s): K. Masuda, N. Sakiyama, R. Tanaka, K. Noguchi, K. Tanaka
  • Journal Title: J. Am. Chem. Soc Volume: 133 Issue: 18 Pages: 6918-6921
  • DOI: 10.1021/ja201337x
  • Peer Reviewed
• [Journal Article] Rhodium-Catalyzed Asymmetric Formal Olefination or Cycloaddition: 1,3-Dicarbonyl Compounds Reacting with 1,6-Diynes or 1,6-Enynes (2011)
  • Author(s): T. Suda, K. Noguchi, K. Tanaka
  • Journal Title: Angew. Chem. Int. Ed Volume: 50 Issue: 19 Pages: 4475-4479
  • DOI: 10.1002/anie.201007727
  • Peer Reviewed
• [Presentation] 遺跡劣化砂岩から分離されたFusarium solaniに内生する細菌 (2014)
  • Author(s): 片山葉子
  • Organizer: 第1回菌類内生細菌情報交換会
  • Place of Presentation: 茨城大学(茨城県)
  • Year and Date: 2014-03-21
  • Invited
• [Presentation] 津波堆積物の性状と微生物叢の好気および嫌気環境下での変化 (2014)
  • Author(s): 猪原英之、堀知行、髙崎みつる、 片山葉子
  • Organizer: 第48回日本水環境学会年会
  • Place of Presentation: 東北大学（宮城県）
• [Presentation] 遺跡劣化砂岩から分離されたFusarium solaniに内生する細菌 (2014)
  • Author(s): 片山葉子
  • Organizer: 第1回菌類内生細菌情報交換会
  • Place of Presentation: 茨城大学（茨城県）
  • Invited
• [Presentation] 土壌より分離した細菌による硫化カルボニル分解 (2013)
  • Author(s): 猪俣祥
  • Organizer: 第29回日本微生物生態学会大会
  • Place of Presentation: 鹿児島大学(鹿児島県)
  • Year and Date: 2013-11-24
• [Presentation] 大気微量硫黄成分である硫化カルボニルの Trichoderma spp.による分解 (2013)
  • Author(s): 小澤理恵
  • Organizer: 第29回日本微生物生態学会大会
  • Place of Presentation: 鹿児島大学(鹿児島県)
  • Year and Date: 2013-11-24
• [Presentation] アンコール遺跡劣化石材表面のバイオフィルムに蓄積した硝酸とその起源の推定 (2013)
  • Author(s): 片山葉子
  • Organizer: 第29回日本微生物生態学会大会
  • Place of Presentation: 鹿児島大学(鹿児島県)
  • Year and Date: 2013-11-24
• [Presentation] Degradation of hedoro, organically enriched sediment, discharged by tsunami of the great east Japan earthquake in 2011 (2013)
  • Author(s): H IHARA
  • Organizer: The 22th Korea-Japan Symposium on Water Environment 2013
  • Place of Presentation: グランドホテル テグ(大韓民国)
  • Year and Date: 2013-10-21
• [Presentation] 森林および火山ガス発生地域の土壌から分離された真菌の硫化カルボニル分解活性の比較 (2013)
  • Author(s): 小澤理恵
  • Organizer: 日本土壌微生物学会2013年度大会
  • Place of Presentation: 東京農工大学(東京都)
  • Year and Date: 2013-06-20
• [Presentation] 遺跡劣化砂岩から分離されたFusarium solani THIF01f 株の無機塩硫黄培地での生育 (2013)
  • Author(s): 北原直樹
  • Organizer: 日本土壌微生物学会 2013年度大会
  • Place of Presentation: 東京農工大学(東京都)
  • Year and Date: 2013-06-20
• [Presentation] 津波堆積物の性状と微生物叢の好気および嫌気環境下での変化 (2013)
  • Author(s): 猪原英之
  • Organizer: 第48回日本水環境学会年会
  • Place of Presentation: 東北大学(仙台市)
  • Year and Date: 2013-03-19
• [Presentation] 森林および火山ガス発生地域の土壌から分離された真菌の硫化カルボニル分解活性の比較 (2013)
  • Author(s): 小澤理恵、猪俣 祥、片山葉子
  • Organizer: 日本土壌微生物学会2013年度大会
  • Place of Presentation: 東京農工大学（東京都）
• [Presentation] 遺跡劣化砂岩から分離されたFusarium solani THIF01f株の無機塩硫黄培地での生育 (2013)
  • Author(s): 北原直樹、大岩祐児、李 賢淑、 Gu Ji-Dong、片山葉子
  • Organizer: 日本土壌微生物学会2013年度大会
  • Place of Presentation: 東京農工大学（東京都）
• [Presentation] Degradation of hedoro, organically enriched sediment, discharged by tsunami of the great east Japan earthquake in 2011 (2013)
  • Author(s): Hideyuki IHARA, Tomoyuki HORI, Mitsuru TAKASAKI, Yoko KATAYAMA
  • Organizer: The 22th Korea-Japan Symposium on Water Environment 2013
  • Place of Presentation: グランドホテル テグ（テグ市、大韓民国）
• [Presentation] 土壌より分離した細菌による硫化カルボニル分解 (2013)
  • Author(s): 猪俣 祥、片山葉子
  • Organizer: 第29回日本微生物生態学会大会
  • Place of Presentation: 鹿児島大学（鹿児島県）
• [Presentation] 大気微量硫黄成分である硫化カルボニルのTrichoderma spp.による分解 (2013)
  • Author(s): 小澤理恵、片山葉子
  • Organizer: 第29回日本微生物生態学会大会
  • Place of Presentation: 鹿児島大学（鹿児島県）
• [Presentation] アンコール遺跡劣化石材表面のバイオフィルムに蓄積した硝酸とその起源の推定 (2013)
  • Author(s): 林フェンユー、久住朝子、 Gu Ji-Dong、染谷 孝、勝山千恵、 諏訪祐一、木庭啓介、片山葉子
  • Organizer: 第29回日本微生物生態学会大会
  • Place of Presentation: 鹿児島大学（鹿児島県）
• [Presentation] Microbial deterioration of stones at Angkor site, Cambodia (2012)
  • Author(s): Y. Katayama
  • Organizer: International Symposium on the Conservation and Restoration of Cultural Property 2012
  • Place of Presentation: 上野(東京都)
  • Year and Date: 2012-12-05
  • Invited
• [Presentation] Oxidation of elemental sulfur and chemolithotrophic growth of Fusarium solani strain THIF01 isolated from deteriorated stone of Angkor monuments (2012)
  • Author(s): N. Kitahara
  • Organizer: International Symposium on the Conservation and Restoration of Cultural Property 2012
  • Place of Presentation: 上野(東京都)
  • Year and Date: 2012-12-05
• [Presentation] Degradation of atmospheric COS by Trichoderma sp. strain KSF8 (2012)
  • Author(s): R Ozawa
  • Organizer: 第28回日本微生物生態学会大会
  • Place of Presentation: 豊橋技術科学大学(愛知県)
  • Year and Date: 2012-09-10
• [Presentation] Microbial growth and oxidation of elemental sulfur on deteriorated sandstones in Angkor monuments, Cambodia (2012)
  • Author(s): Y. Katayama
  • Organizer: Chemical and ecological researches on the microorganisms for preserving cultural properties in both Japan and Hungary
  • Place of Presentation: 奈良市(奈良県)
  • Year and Date: 2012-07-07
  • Invited
• [Presentation] Carbonyl sulfide hydrolase : a novel β-carbonic anhydrase family enzyme catalyzing ambient level of carbonyl sulfide (2012)
  • Author(s): T Ogawa
  • Organizer: 12th Japan-China-Korea Joint Symposium on Enzyme Engineering(日中韓酵素工学会議)
  • Place of Presentation: (日中韓酵素工学会議)
  • Year and Date: 2012-05-28
• [Presentation] Degradation of atmospheric COS by Trichoderma sp. strain KSF8 (2012)
  • Author(s): R. Ozawa, Y. Katayama
  • Organizer: 第２７回日本微生物生態学会大会
  • Place of Presentation: 豊橋、愛知県、日本
• [Presentation] Carbonyl sulfide hydroase:a novel β-carbonic anhydrase family enzyme catalyzing ambient level of carbonil sulfide (2012)
  • Author(s): T. Ogawa, K. Noguchi, M. Odaka, Y. Katayama
  • Organizer: 12th Japan-China-Korea Joint Symposium on Enzyme Enginnering（日中韓酵素工学会議）
  • Place of Presentation: 金沢、石川県、日本
• [Presentation] Microbial deterioration of stones at Angkor site, Cambodia. (2012)
  • Author(s): Y. Katayama
  • Organizer: International Symposium on the Conservation and Restoration of Cultural Property 2012.
  • Place of Presentation: 上野、東京都、日本
  • Invited
• [Presentation] Microbial growth and oxidation of elemental sulfur on deteriorated sandstones in Angkor monuments, Cambodia. (2012)
  • Author(s): Y. Katayama
  • Organizer: Chemical and ecological researches on the microorganisms for preserving cultural properties in both Japan and Hungary.
  • Place of Presentation: 奈良市、奈良県、日本
  • Invited
• [Presentation] Oxidation of elemental sulfur and chemolithotrophic growth of Fusarium solani strain THIF01 isolated from deteriorated stone of Angkor monuments. (2012)
  • Author(s): N. Kitahara, Y. Katayama
  • Organizer: International Symposium on the Conservation and Restoration of Cultural Property 2012.
  • Place of Presentation: 上野、東京都、日本
• [Presentation] 富士山頂で採取した自由対流圏を長距離輸送されたバイオエアロゾルに含まれる微生物の特徴 (2011)
  • Author(s): 忠地駿、久住朝子、富田新、大須賀雄、小林拓、片山葉子
  • Organizer: 第27回日本微生物生態学会大会
  • Place of Presentation: 京都大学農学部総合館
  • Year and Date: 2011-10-08
• [Presentation] アンコール遺跡における剥離劣化石材と着色劣化石材の細菌群集構造の比較 (2011)
  • Author(s): 久住朝子、李賢淑、大須賀雄、大岩祐児、富田新、片山葉子
  • Organizer: 第27回日本微生物生態学会大会
  • Place of Presentation: 京都大学農学部総合館
  • Year and Date: 2011-10-08
• [Presentation] Siphonobacter sp.THI313株の辛味ダイコン由来チオシアネート配糖体の分解とそれに伴うCOSの生成 (2011)
  • Author(s): 坂本友希子、関田真奈、高田保孝、片山葉子、芥川一成、千葉一裕
  • Organizer: 第27回日本微生物生態学会大会
  • Place of Presentation: 京都大学農学部総合館
  • Year and Date: 2011-10-08
• [Presentation] Gene cloning of enzyme thiocyanate hydrolase of strain THI201 (2011)
  • Author(s): Adeeba Hussain, Y.Katayama, Y.Matsushita
  • Organizer: International Union of Microbiological Societies 2011 Congress
  • Place of Presentation: Sapporo, Japan. Sapporo Convention Center
• [Presentation] Bacterial community analyses of biofilms on the deteriorated sandstone of Bayon temple of Angkor monuments in Cambodia (2011)
  • Author(s): Asako Kusumi, Xian-Shu Li, Yu Osuga, Ji-Dong Gu, Yoko Katayama
  • Organizer: 15^<th> International Biodeterioration and Biodegradation Symposium
  • Place of Presentation: Univ.of Natural Resources and Life Sciences, Vienna, Austria
• [Presentation] Thiobacillus thioparus THI115の硫化カルボニル(COS)加水分解酵素のCOS分解特性およびカルボニックアンヒドラーゼ(CA)との比較 (2011)
  • Author(s): 小川貴弘、野口恵一、尾高雅文、養王田正文、片山葉子
  • Organizer: 環境バイオテクノロジー学会2011年度大会
  • Place of Presentation: 東京大学弥生講堂
• [Book] よみがえる富士山測候所 2012年 (2012)
  • Author(s): 大河内博,緒方裕子,皆巳幸也,片山葉子,米持真一
  • Publisher: 成山堂書店
• [Book] よみがえる富士山測候所２０１２年 (2012)
  • Author(s): 土器屋由紀子
  • Total Pages: 180
  • Publisher: 成山堂書店
• [Book] 環境毒性学 (2011)
  • Author(s): 片山葉子(分担)
  • Total Pages: 252
  • Publisher: 朝倉書店
• [Remarks] 国立大学法人東京農工大学科学技術展2013土壌に生息する微生物による空気中の硫化カルボニルの分解 猪俣祥,小澤理恵,片山葉子
• [Remarks] 国立大学法人東京農工大学科学技術展2012 土壌微生物のはたらき‐空気中の微量硫黄ガスの分解‐小澤理恵,猪俣祥,加藤広海,片山葉子
• [Remarks] 国立大学法人東京農工大学科学技術展2011 効率的除去を目指した新奇硫化カルボニル分解酵素の高機能化 片山葉子,野口恵一,小川貴弘,尾高雅文