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Measurement of local viscosity of the hydration shell of protein by time-resolved fluorescence anisotropy spectroscopy

Research Project

Project/Area Number 23310071
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Research Field Nanomaterials/Nanobioscience
Research InstitutionTohoku University

Principal Investigator

SUZUKI MAKOTO  東北大学, 工学(系)研究科(研究院), 教授 (60282109)

Co-Investigator(Kenkyū-buntansha) MORIMOTO Nobuyuki  東北大学, 大学院工学研究科, 准教授 (00313263)
和沢 鉄一  東北大学, 工学(系)研究科(研究院), 研究支援者 (80359851)
Co-Investigator(Renkei-kenkyūsha) WAZAWA Tetsuichi  東北大学, 大学院工学研究科, 研究支援者 (80359851)
Project Period (FY) 2011-04-01 – 2014-03-31
Project Status Completed (Fiscal Year 2013)
Budget Amount *help
¥19,500,000 (Direct Cost: ¥15,000,000、Indirect Cost: ¥4,500,000)
Fiscal Year 2013: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
Fiscal Year 2012: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
Fiscal Year 2011: ¥15,080,000 (Direct Cost: ¥11,600,000、Indirect Cost: ¥3,480,000)
Keywords局所粘度計測 / 蛍光偏光解消 / 回転相関時間 / ハイパーモバイル水 / アクチンフィラメント / 蛍光異方性 / 水和水 / 水和水の粘度 / ハイパーモバイル水が
Research Abstract

In this study, rotational correlation time Tc of fluorescent dye was measured to evaluate the local viscosity of the hydration region of protein surface.The Tc measured by this method was found to be in linear relation with the solvent viscosity. The CW-laser-based method revealed shorter Tc of Cy3 linked at Cys374 of actin in F-actin than around G-actin, which is consistent with the result by dielectric relaxation data.On the other hand,Tc (<1ns) of small fluorescent dye such as rhodamin 6G in water could not be measured by pulse-laser-based method without picosecond pulse width.We have designed a frequency-region fluorescent anisotropy spectroscopic method to measure Tc of small fluorescent dyes.

Report

(4 results)
  • 2013 Annual Research Report   Final Research Report ( PDF )
  • 2012 Annual Research Report
  • 2011 Annual Research Report
  • Research Products

    (74 results)

All 2014 2013 2012 2011 Other

All Journal Article (10 results) (of which Peer Reviewed: 10 results) Presentation (56 results) (of which Invited: 10 results) Book (1 results) Remarks (3 results) Patent(Industrial Property Rights) (4 results)

  • [Journal Article] Comparative Study on the Properties of Hydration Water of Na- and K-Halide Ions by Raman OH/OD-stretching Spectroscopy and Dielectric Relaxation Data2014

    • Author(s)
      Yuichiro Okazaki, Tetsuo Taniuchi, George Mogami, Nobuyuki Matubayasi and Makoto Suzuki
    • Journal Title

      J. Phys. Chem. A

      Volume: 118 Issue: 16 Pages: 2922-2930

    • DOI

      10.1021/jp412804d

    • Related Report
      2013 Annual Research Report 2013 Final Research Report
    • Peer Reviewed
  • [Journal Article] What is “hypermobile” water? :detected in alkali halide, adenosine phosphate and F-actin solutions by high-resolution microwave dielectric spectroscopy2014

    • Author(s)
      Makoto Suzuki
    • Journal Title

      Pure and Applied Chemistry

      Volume: 86 Issue: 2 Pages: 181-189

    • DOI

      10.1515/pac-2014-5024

    • Related Report
      2013 Annual Research Report 2013 Final Research Report
    • Peer Reviewed
  • [Journal Article] 1,3-Diethylurea-enhanced Mg-ATPase activity of skeletal muscle myosin with a converse effect on the sliding motility2013

    • Author(s)
      Tetsuichi Wazawa, Shin-ichiro Yasui, Nobuyuki Morimoto, Makoto Suzuki
    • Journal Title

      Biochimica et Biophysica Acta (BBA) - Proteins and Proteomics

      Volume: 1834 Issue: 12 Pages: 2620-2629

    • DOI

      10.1016/j.bbapap.2013.08.003

    • Related Report
      2013 Annual Research Report 2013 Final Research Report
    • Peer Reviewed
  • [Journal Article] Anion-Dependence of Fast Relaxation Component in Na-, K-Halide 2 Solutions at Low Concentrations Measured by High-Resolution 3 Microwave Dielectric Spectroscopy2013

    • Author(s)
      George Mogami, Takashi Miyazaki, Tetsuichi Wazawa, Nobuyuki Matubayasi, and Makoto Suzuki
    • Journal Title

      J. Phys. Chem A

      Volume: 117 Issue: 23 Pages: 4851-4862

    • DOI

      10.1021/jp4012119

    • Related Report
      2013 Annual Research Report 2013 Final Research Report
    • Peer Reviewed
  • [Journal Article] Hydration state change of horse heart cytochrome c corresponding to trifluoroacetic-acid-induced unfolding2013

    • Author(s)
      Yusuke Miyashita, Tetsuichi Wazawa, George Mogami, Satoshi Takahashi, Yoshihiro Sambongi, and Makoto Suzuki
    • Journal Title

      Biophysical Journal

      Volume: 104 Issue: 1 Pages: 163-172

    • DOI

      10.1016/j.bpj.2012.11.3825

    • Related Report
      2013 Annual Research Report 2012 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Role of Water in Actin-Myosin Binding and Actin Polymerization : Rotational and Translational Mobility of Water Molecules2012

    • Author(s)
      Makoto Suzuki, Tetsuichi Wazawa, George Mogami, and Takao Kodama
    • Journal Title

      Journal of the Physical Society of Japan, Supplement

      Volume: 81 Issue: Suppl.A Pages: SA003-SA003

    • DOI

      10.1143/jpsjs.81sa.sa003

    • NAID

      40019408743

    • Related Report
      2013 Annual Research Report 2013 Final Research Report
    • Peer Reviewed
  • [Journal Article] Molecular dynamics study of fast dielectric relaxation of water around a molecular-sized ion2012

    • Author(s)
      Y. Kubota, A. Yoshimori, N. Matubayasi, M. Suzuki, and R. Akiyama
    • Journal Title

      J. Chem. Phys

      Volume: 137 Issue: 22

    • DOI

      10.1063/1.4769972

    • Related Report
      2012 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Role of Water in Actin–Myosin Binding and Actin Polymerization: Rotational and Translational Mobility of Water Molecules2012

    • Author(s)
      Makoto Suzuki, Tetsuichi Wazawa, George Mogami, and Takao Kodama
    • Journal Title

      J. Phys. Soc. Jpn.

      Volume: 81

    • NAID

      40019408743

    • Related Report
      2012 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Role of Water in Actin-Myosin Binding and Actin Polymerization : Rotational and Translational Mobility of Water Molecules2012

    • Author(s)
      Makoto Suzuki, Tetsuichi Wazawa, George Mogami, Takao Kodama
    • Journal Title

      Journal of the Physical Societu of Japan

      Volume: Supplement(In press)

    • NAID

      40019408743

    • Related Report
      2011 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Hyper-mobility of water around actin filaments revealed using pulse-field gradient spin-echo 1H-NMR and fluorescence spectroscopy2011

    • Author(s)
      Tetsuichi Wazawa, Takashi Sagawa, Tsubasa Ogawa, Nobuyuki Morimoto, Makoto Suzuki
    • Journal Title

      Biochem.Biophys.Res.Comm.

      Volume: 404 Issue: 4 Pages: 985-990

    • DOI

      10.1016/j.bbrc.2010.12.096

    • Related Report
      2013 Final Research Report 2011 Annual Research Report
    • Peer Reviewed
  • [Presentation] Rotational correlation time of a fluorophore tethered to actin as studied by frequency-domain fluorescence anisotropy measurements2013

    • Author(s)
      Tetsuichi Wazawa, Nobuyuki Morimoto, Makoto Suzuki
    • Organizer
      51st Ann. Meeting Biophys. Soc. Jpn
    • Place of Presentation
      Kyoto
    • Related Report
      2013 Final Research Report
  • [Presentation] 1,3-Diethylurea-enhanced Mg-ATPase of skeletal muscle myosin with a converse effect on theslidingmotility2013

    • Author(s)
      Tetsuichi Wazawa, Shin-ichiro Yasui, Nobuyuki Morimoto, Makoto Suzuki
    • Organizer
      51st Ann. Meeting Biophys. Soc. Jpn
    • Place of Presentation
      Kyoto
    • Related Report
      2013 Final Research Report
  • [Presentation] Hydration properties of sodium-oligophosphates, -alkyl carboxylates and -alkyl sulfonates by dielectric relaxation spectroscopy2013

    • Author(s)
      Kazuki Ishimori, Yangtian Wang, Norihiko Tanno, George Mogami, Tetsuichi Wazawa, Nobuyuki Morimoto, Makoto Suzuki
    • Organizer
      51st Ann. Meeting Biophys. Soc. Jpn
    • Place of Presentation
      Kyoto
    • Related Report
      2013 Final Research Report
  • [Presentation] Hydration and partial specific volume measurements of G-and F-actin2013

    • Author(s)
      Asato Imao, Takahiro Watanabe, Tetsuichi Wazawa, George Mogami, Nobuyuki Morimoto, Makoto Suzuki
    • Organizer
      51st Ann. Meeting Biophys. Soc. Jpn
    • Place of Presentation
      Kyoto
    • Related Report
      2013 Final Research Report
  • [Presentation] Halide ion effect on hydration state of F-actin2013

    • Author(s)
      Noriyoshi Ishida, Takahiro Watanabe, George Mogami, Tetsuichi Wazawa, Makoto Suzuki
    • Organizer
      51st Ann. Meeting Biophys. Soc. Jpn
    • Place of Presentation
      Kyoto
    • Related Report
      2013 Final Research Report
  • [Presentation] Hydration study of adenosine phosphates(ATP) and ATP-driven proteins by dielectric relaxation spectroscopy2013

    • Author(s)
      M. Suzuki, G. Mogami, A. Imao, T. Wazawa, N. Morimoto
    • Organizer
      33rd Int. Conf. of Solution Chemistry
    • Place of Presentation
      京都
    • Related Report
      2013 Annual Research Report
    • Invited
  • [Presentation] 水を主役としたATPエネルギー変換2013

    • Author(s)
      鈴木 誠
    • Organizer
      第3回CSJフェスタ
    • Place of Presentation
      東京
    • Related Report
      2013 Annual Research Report
    • Invited
  • [Presentation] Rotational correlation time of a fluorophore- tethered G actin as studied by frequency-domain fluorescence anisotropy measurements2013

    • Author(s)
      T. Wazawa,N. Morimoto,M. Suzuki
    • Organizer
      51st Ann. Meeting of Biophys. Soc. Jpn
    • Place of Presentation
      京都
    • Related Report
      2013 Annual Research Report
  • [Presentation] 1,3-Diethylurea-enhancedMg-ATPase of skeletal musde myosin wilh a converse effect on the sliding motility2013

    • Author(s)
      T. Wazawa, S. Yasui,N. Morimoto,M. Suzuki
    • Organizer
      51st Ann. Meeting of Biophys. Soc. Jpn
    • Place of Presentation
      京都
    • Related Report
      2013 Annual Research Report
  • [Presentation] Effects of halide ions on the hydration properties of F-actin, Effects of nucleotide and cosolvents on the hydration state of myosin subfragment-12013

    • Author(s)
      鈴木 誠
    • Organizer
      新学術領域研究「水を主役としたATPエネルギー変換」国際シンポジウム
    • Place of Presentation
      仙台秋保 岩沼屋
    • Related Report
      2012 Annual Research Report
    • Invited
  • [Presentation] Hypermobile water observed in sulfonates, phosphates solutions and its volume change in protein interactions2013

    • Author(s)
      鈴木 誠
    • Organizer
      新学術領域研究「水を主役としたATPエネルギー変換」国際シンポジウム
    • Place of Presentation
      仙台秋保 岩沼屋
    • Related Report
      2012 Annual Research Report
    • Invited
  • [Presentation] Anion-dependence of hypermobile water in Na-, K-halide solutions at low concentrations measured by high-resolution microwave dielectric spectroscopy2013

    • Author(s)
      鈴木 誠
    • Organizer
      新学術領域研究「水を主役としたATPエネルギー変換」国際シンポジウム
    • Place of Presentation
      仙台秋保 岩沼屋
    • Related Report
      2012 Annual Research Report
    • Invited
  • [Presentation] Hydration and ATP energetic on Actomyosin2013

    • Author(s)
      鈴木 誠
    • Organizer
      Alpbach Workshop on Molecular Motors 2013
    • Place of Presentation
      Alpbach, Austria
    • Related Report
      2012 Annual Research Report
  • [Presentation] マイクロ波誘電緩和分光法による有機・無機イオンおよびタンパク質の水和特性2012

    • Author(s)
      鈴木誠
    • Organizer
      日本分光学会テラヘルツ分光部会シンポジウム「テラヘルツ分光法の最先端VI~ここまできたテラヘルツ時間領域分光~」
    • Place of Presentation
      千葉
    • Year and Date
      2012-10-26
    • Related Report
      2013 Final Research Report
    • Invited
  • [Presentation] Temperature dependence of hydration properties of F-actin by die1eclectric re1axation spectroscopy2012

    • Author(s)
      Yuichiro Okazaki, Asato Imao, Noriyoshi Ishida, George Mogami, Tetsuichi Wazawa, Makoto Suzuki
    • Organizer
      50th Ann. Meeting of Biophys. Soc. Jpn
    • Place of Presentation
      Nagoya
    • Related Report
      2013 Final Research Report
  • [Presentation] Dielectrie analysis of hydration change of actin by polymerization2012

    • Author(s)
      Alato Imao, Yuichiro Okazaki, Noriyoshi Ishida, Tetsuichi Wazawa. George Mogami. Makoto Suzuki (Grad. Sch. Eng., Tohoku. Univ)
    • Organizer
      50th Ann. Meeting of Biophys. Soc. Jpn
    • Place of Presentation
      Nagoya
    • Related Report
      2013 Final Research Report
  • [Presentation] Effects of halide ions on the hydration properties of F-actin2012

    • Author(s)
      Noriyoshi Ishida, Asato Imao, Yuichiro Okazaki, Tetsuichi Wazawa, George Mogami, Makoto Suzuki
    • Organizer
      50th Ann. Meeting of Biophys. Soc. Jpn
    • Place of Presentation
      Nagoya
    • Related Report
      2013 Final Research Report
  • [Presentation] Cosolvent effects on the hydration state and the enzymatie activity of skeletal myosin2012

    • Author(s)
      Takuya Nakagawa, Shin-ichiro Yasui., Tetsuichi Wazawa, George Mogami, Makoto Suzuki
    • Organizer
      50th Ann. Meeting of Biophys. Soc. Jpn
    • Place of Presentation
      Nagoya
    • Related Report
      2013 Final Research Report
  • [Presentation] Hydration study of oligophosphates by dielectric relaxation specytroscopy2012

    • Author(s)
      Kazuki Ishimori, George Mogami, Nobuyuki Morimoto, Makoto Suzuki
    • Organizer
      50th Ann. Meeting of Biophys. Soc. Jpn
    • Place of Presentation
      Nagoya
    • Related Report
      2013 Final Research Report
  • [Presentation] アクチンフィラメント周囲の高い運動性を有する水和水の蛍光法による検出2012

    • Author(s)
      和沢鉄一,最上譲二,佐川貴史,森本展行,鈴木誠
    • Organizer
      第12回日本蛋白質科学会年会
    • Place of Presentation
      名古屋
    • Related Report
      2013 Final Research Report
  • [Presentation] アクチン重合におけるハイパーモバイル水の役割について考える2012

    • Author(s)
      鈴木誠
    • Organizer
      2012年生体運動研究合同班会議
    • Place of Presentation
      筑波大学
    • Related Report
      2011 Annual Research Report
  • [Presentation] ATP加水分解およびATP駆動タンパク質のエネルギー・水和状態相関解析2012

    • Author(s)
      鈴木誠
    • Organizer
      新学術領域研究「水を主役としたATPエネルギー変換」第4回領域全体会議
    • Place of Presentation
      仙台秋保岩沼屋
    • Related Report
      2011 Annual Research Report
  • [Presentation] アクチンの重合による水和状態の変化およびF-アクチン周りバイパーモバイル水の生成エンタルピー2012

    • Author(s)
      今尾麻人、岡崎裕一郎、和沢鉄一、最上譲二、森本展行、鈴木誠
    • Organizer
      新学術領域研究「水を主役としたATPエネルギー変換」第4回領域全体会議
    • Place of Presentation
      仙台秋保岩沼屋
    • Related Report
      2011 Annual Research Report
  • [Presentation] アクチンの重合・脱重合における熱(DSC, ITC)測定2012

    • Author(s)
      阿部智彦、安居慎一郎、和沢鉄一、最上譲二、森本展行、鈴木誠
    • Organizer
      新学術領域研究「水を主役としたATPエネルギー変換」第4回領域全体会議
    • Place of Presentation
      仙台秋保岩沼屋
    • Related Report
      2011 Annual Research Report
  • [Presentation] ミオシンS1のATPase活性およびヌクレオチド結合熱におよぼす尿素および尿素誘導体の効果2012

    • Author(s)
      安居慎一郎、阿部智彦、和沢鉄一、最上譲二、鈴木誠
    • Organizer
      新学術領域研究「水を主役としたATPエネルギー変換」第4回領域全体会議
    • Place of Presentation
      仙台秋保岩沼屋
    • Related Report
      2011 Annual Research Report
  • [Presentation] 尿素および尿素誘導体水溶液の誘電緩和特性の温度依存性2012

    • Author(s)
      小野祐貴、和沢鉄一、最上譲二、森本展行、鈴木誠
    • Organizer
      新学術領域研究「水を主役としたATPエネルギー変換」第4回領域全体会議
    • Place of Presentation
      仙台秋保岩沼屋
    • Related Report
      2011 Annual Research Report
  • [Presentation] アクチンおよびミオシンS1の水和状態に及ぼす尿素および尿素誘導体の効果2012

    • Author(s)
      中川卓哉、石田誠宜、安居慎一郎、和沢鉄一、最上譲二、鈴木誠
    • Organizer
      新学術領域研究「水を主役としたATPエネルギー変換」第4回領域全体会議
    • Place of Presentation
      仙台秋保岩沼屋
    • Related Report
      2011 Annual Research Report
  • [Presentation] カルボン酸の水和におよぼすアルキル基の効果2012

    • Author(s)
      王楊天、最上譲二、和沢鉄一、森本展行、鈴木誠
    • Organizer
      新学術領域研究「水を主役としたATPエネルギー変換」第4回領域全体会議
    • Place of Presentation
      仙台秋保岩沼屋
    • Related Report
      2011 Annual Research Report
  • [Presentation] アクチンフィラメントの重合による周りの水の物性変化と熱の出入り2012

    • Author(s)
      鈴木誠
    • Organizer
      「水和とATP」サテライトミーティング"蛋白質折り畳みの理解に向けた一分子基礎科学を目指して"
    • Place of Presentation
      仙台作並一の坊
    • Related Report
      2011 Annual Research Report
  • [Presentation] 蛍光スペクトルで観るアクチンフィラメント周りの水のハイパーモビリティ2012

    • Author(s)
      和沢鉄一
    • Organizer
      「水和とATP」サテライトミーティング"蛋白質折り畳みの理解に向けた一分子基礎科学を目指して"
    • Place of Presentation
      仙台作並一の坊
    • Related Report
      2011 Annual Research Report
  • [Presentation] 誘電緩和スペクトルの緩和成分解析からわかること2012

    • Author(s)
      最上譲二
    • Organizer
      「水和とATP」サテライトミーティング"蛋白質折り畳みの理解に向けた一分子基礎科学を目指して"
    • Place of Presentation
      仙台作並一の坊
    • Related Report
      2011 Annual Research Report
  • [Presentation] アクチンの周りの水の回転・並進運動性と局所粘度2011

    • Author(s)
      鈴木誠
    • Organizer
      平成23年度「筋生理の集い」
    • Place of Presentation
      東京慈恵医科大学
    • Year and Date
      2011-12-17
    • Related Report
      2011 Annual Research Report
  • [Presentation] Overview of studies on ATP-driven proteins and asymmetry2011

    • Author(s)
      Makoto Suzuki
    • Organizer
      Symposium " Asymmetry produced by water and ATP",第49回日本生物物理学会年会
    • Place of Presentation
      姫路
    • Year and Date
      2011-09-16
    • Related Report
      2013 Final Research Report
    • Invited
  • [Presentation] Overview of studies on ATP-driven proteins and asymmetry, Symposium "Asymmetry produced by water and ATP"2011

    • Author(s)
      鈴木誠
    • Organizer
      第49回日本生物物理学会年会
    • Place of Presentation
      兵庫県立大学(招待)
    • Year and Date
      2011-09-16
    • Related Report
      2011 Annual Research Report
  • [Presentation] アクチンフィラメントの一方向的な構造変化:ミオシン力発生機構への関与の可能性2011

    • Author(s)
      上田太郎、鈴木誠
    • Organizer
      第49回日本生物物理学会年会
    • Place of Presentation
      兵庫県立大学(招待)
    • Year and Date
      2011-09-16
    • Related Report
      2011 Annual Research Report
  • [Presentation] ATP加水分解過程におけるタンパク質表面の水の状態の変化2011

    • Author(s)
      鈴木誠
    • Organizer
      シンポジウム「蛋白質/水界面の熱力学とATPエネルギー」第63回コロイドおよび界面化学討論会
    • Place of Presentation
      京都
    • Year and Date
      2011-09-07
    • Related Report
      2013 Final Research Report
    • Invited
  • [Presentation] ATP加水分解過程におけるタンパク質表面の水の状態の変化、シンポジウム「蛋白質/水界面の熱力学とATPエネルギー」2011

    • Author(s)
      鈴木誠
    • Organizer
      第63回コロイドおよび界面化学討論会
    • Place of Presentation
      京都(招待)
    • Year and Date
      2011-09-07
    • Related Report
      2011 Annual Research Report
  • [Presentation] ATP加水分解およびATP駆動タンパク質のエネルギー・水和状態相関解析2011

    • Author(s)
      鈴木誠
    • Organizer
      新学術領域研究「水を主役としたATPエネルギー変換」の領域研究の活動として、全体会議後期(2011-2012)研究計画
    • Place of Presentation
      大阪ガーデンパレス
    • Year and Date
      2011-08-11
    • Related Report
      2011 Annual Research Report
  • [Presentation] Hyper-mobility of water around actin filaments revealed using pulse-field gradient spin-echo 1H NMR and fluorescence spectroscopy2011

    • Author(s)
      Tetsuichi Wazawa, Takashi Sagawa, Tsubasa Ogawa, Nobuyuki Morimoto, Takao Kodama, Makoto Suzuki
    • Organizer
      新学術領域研究「水を主役としたATPエネルギー変換」の領域研究の活動として、全体会議後期(2011-2012)研究計画
    • Place of Presentation
      大阪ガーデンパレス
    • Year and Date
      2011-08-11
    • Related Report
      2011 Annual Research Report
  • [Presentation] Actin polymerization induces hyper-mobility of surrounding water molecules2011

    • Author(s)
      Tetsuichi Wazawa, Takashi Sagawa, Tsubasa Ogawa, Nobuyuki Morimoto, Makoto Suzuki
    • Organizer
      日本生物物理学会東北支部講演会
    • Place of Presentation
      東北大学片平キャンパス(招待)
    • Year and Date
      2011-06-17
    • Related Report
      2011 Annual Research Report
  • [Presentation] Role of water in actin-myosin binding and actin polymerization : rotational and translational mobility of water molecules2011

    • Author(s)
      Makoto Suzuki, Tetsuichi Wazawa, George Mogami, Takao Kodama
    • Organizer
      5th Mini-Symposium on Liquids . Fundamental problems on liquids and related topics-
    • Place of Presentation
      Okayama
    • Related Report
      2013 Final Research Report
    • Invited
  • [Presentation] ポリ(アミドアミン)デンドリマの水和状態解析2011

    • Author(s)
      森本展行、小川翼、最上譲二、鈴木誠
    • Organizer
      第60回高分子学会年次大会
    • Place of Presentation
      大阪
    • Related Report
      2011 Annual Research Report
  • [Presentation] 誘電緩和分光法による水和測定の分解能の向上と信頼性の検証2011

    • Author(s)
      George Mogami, Tetsuichi Wazawa, Makoto Suzuki
    • Organizer
      第49回日本生物物理学会年会
    • Place of Presentation
      兵庫県立大学
    • Related Report
      2011 Annual Research Report
  • [Presentation] Effects of Urea and Its Derivatives on the Water Dynamics as studied by microwave dielectric relaxation Spectroscopy2011

    • Author(s)
      Batnasan Altansukh, Tetsuichi Wazawa, George Mogami, Nobuyuki Morimoto, Makoto Suzuki
    • Organizer
      第49回日本生物物理学会年会
    • Place of Presentation
      兵庫県立大学
    • Related Report
      2011 Annual Research Report
  • [Presentation] ADPとミオシンS1の結合熱に及ぼす尿素とその誘導体の影響2011

    • Author(s)
      Tomohiko Abe, Altansukh Batnasan, Shin-ichiro Yasui, George Mogami, Makoto Suzuki
    • Organizer
      第49回日本生物物理学会年会
    • Place of Presentation
      兵庫県立大学
    • Related Report
      2011 Annual Research Report
  • [Presentation] Hyper-mobility of water around actin filaments studied by pulse-field gradient spin-echo 1H NMR and fluorescence spectroscopy2011

    • Author(s)
      Tetsuichi Wazawa, Takashi Sagawa, Tsubasa Ogawa, Nobuyuki Morimoto, Takao Kodama, Makoto Suzuki
    • Organizer
      第49回日本生物物理学会年会
    • Place of Presentation
      兵庫県立大学
    • Related Report
      2011 Annual Research Report
  • [Presentation] 尿素及び尿素誘導体のミオシンATP加水分解活性に及ぼす効果2011

    • Author(s)
      安居慎一郎, 和沢鉄一, Batnasan Altansukh, 石田誠宜, 中川卓哉, 鈴木誠
    • Organizer
      第49回日本生物物理学会年会
    • Place of Presentation
      兵庫県立大学
    • Related Report
      2011 Annual Research Report
  • [Presentation] Local viscosity decrease of water surrounding actin upon pplymerization detected by dielectric relaxation, PFGSE-NMR, and fluorescence anisotropy measurements2011

    • Author(s)
      鈴木誠
    • Organizer
      The 5th international mini-symposium on liquids
    • Place of Presentation
      岡山大学創立50周年記念館(招待)
    • Related Report
      2011 Annual Research Report
  • [Presentation] Proton dififusion coeffcient of actin solution measured by PFG-SE H NMR2011

    • Author(s)
      鈴木誠
    • Organizer
      新学術領域研究「水を主役としたATPエネルギー変換」の領域研究の活動として、前期(2008-2010)研究成果報告会
    • Place of Presentation
      大阪ガーデンパレス
    • Related Report
      2011 Annual Research Report
  • [Presentation] ATP加水分解過程における蛋白質表面の水の状態の変化2011

    • Author(s)
      鈴木誠
    • Organizer
      第63回コロイドおよび界面化学討論会「タンパク質/水界面の熱力学とATPエネルギー」シンポジウム
    • Place of Presentation
      京都大学百周年時計台記念館(招待講演)
    • Related Report
      2011 Annual Research Report
  • [Presentation] Hydration Properties of Adenosine Phosphate Series as Studied by Microwave Dielectric Spectroscopy2011

    • Author(s)
      George Mogami, Tetsuichi Wazawa, Nobuyuki Morimoto, Takao Kodama, Makoto Suzuki
    • Organizer
      新学術領域研究「水を主役としたATPエネルギー変換」前期(2008-2010)研究成果報告会
    • Place of Presentation
      大阪ガーデンパレス
    • Related Report
      2011 Annual Research Report
  • [Presentation] Hydration properties of urea, thiourea and alkylurea derivatives as studied by microwave dielectric relaxation Spectroscopy2011

    • Author(s)
      Batnasan Altansukh, George Mogami, Tetsuichi Wazawa, Nobuyuki Morimoto, Makoto Suzuki
    • Organizer
      Grant-in-Aid for Scientific Researcheron Innovative Area : Hydration and ATP Energy
    • Place of Presentation
      大阪ガーデンパレス(poster)
    • Related Report
      2011 Annual Research Report
  • [Presentation] 精密誘電分光測定によるポリ(ビニルホスホン酸)の水和構造解析2011

    • Author(s)
      佐々木優, 丹野則彦, 最上譲二, 和沢鉄一, 森本展行
    • Organizer
      第60回高分子討論会
    • Place of Presentation
      岡山大学
    • Related Report
      2011 Annual Research Report
  • [Presentation] マイクロ波誘電緩和分光法による有機・無機イオンおよびタンパク質の水和特性

    • Author(s)
      鈴木 誠
    • Organizer
      日本分光学会テラヘルツ分光部会シンポジウム「テラヘルツ分光法の最先端VI~ここまできたテラヘルツ時間領域分光~」
    • Place of Presentation
      筑波
    • Related Report
      2012 Annual Research Report
    • Invited
  • [Presentation] 時間分解蛍光偏光法によるタンパク質周囲の水和層の局所粘度変性測定

    • Author(s)
      Tetsuichi Wazawa, George Mogami, Nobuyuki Morimoto, NOriyoshi Ishida, Makoto Suzuki
    • Organizer
      第50回日本生物物理学会年会
    • Place of Presentation
      名古屋大学
    • Related Report
      2012 Annual Research Report
  • [Presentation] 誘電緩和分光法によるアルキルカルボン酸の水和特性

    • Author(s)
      Yangtian Wang, Goerge Mogami, Tetsuichi Wazawa, Nobuyuki Morimoto, Makoto Suzuki
    • Organizer
      第50回日本生物物理学会年会
    • Place of Presentation
      名古屋大学
    • Related Report
      2012 Annual Research Report
  • [Book] アクチュエータ研究開発の最前線第2編第3章第5節2011

    • Author(s)
      鈴木誠
    • Total Pages
      576
    • Publisher
      エヌ・ティー・エス
    • Related Report
      2011 Annual Research Report
  • [Remarks]

    • URL

      http://www.material.tohoku.ac.jp/atpwater/

    • Related Report
      2013 Final Research Report
  • [Remarks] 水を主役としたATPエネルギー変換

    • Related Report
      2012 Annual Research Report
  • [Remarks]

    • URL

      http://www.material.tohoku.ac.jp/atpwater/index.htm

    • Related Report
      2011 Annual Research Report
  • [Patent(Industrial Property Rights)] 周波数領域蛍光測定装置2014

    • Inventor(s)
      和沢鉄一、鈴木誠
    • Industrial Property Rights Holder
      国立大学法人東北大学
    • Industrial Property Rights Type
      特許
    • Filing Date
      2014-01-08
    • Related Report
      2013 Final Research Report
  • [Patent(Industrial Property Rights)] 周波数領域蛍光測定装置2014

    • Inventor(s)
      和沢鉄一、鈴木 誠
    • Industrial Property Rights Holder
      国立大学法人東北大学
    • Industrial Property Rights Type
      特許
    • Industrial Property Number
      2014-001292
    • Filing Date
      2014-01-08
    • Related Report
      2013 Annual Research Report
  • [Patent(Industrial Property Rights)] 周波数領域蛍光測定装置2013

    • Inventor(s)
      和沢鉄一、鈴木誠
    • Industrial Property Rights Holder
      国立大学法人東北大学
    • Industrial Property Rights Type
      特許
    • Filing Date
      2013-09-18
    • Related Report
      2013 Final Research Report
  • [Patent(Industrial Property Rights)] 周波数領域蛍光測定装置2013

    • Inventor(s)
      和沢鉄一、鈴木 誠
    • Industrial Property Rights Holder
      国立大学法人東北大学
    • Industrial Property Rights Type
      特許
    • Industrial Property Number
      2013-192544
    • Filing Date
      2013-09-18
    • Related Report
      2013 Annual Research Report

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Published: 2011-04-06   Modified: 2019-07-29  

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