Measurement of local viscosity of the hydration shell of protein by time-resolved fluorescence anisotropy spectroscopy
| Project/Area Number |
23310071
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Nanomaterials/Nanobioscience
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| Research Institution | Tohoku University |
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Principal Investigator |
SUZUKI MAKOTO 東北大学, 工学(系)研究科(研究院), 教授 (60282109)
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| Co-Investigator(Kenkyū-buntansha) |
MORIMOTO Nobuyuki 東北大学, 大学院工学研究科, 准教授 (00313263)
和沢 鉄一 東北大学, 工学(系)研究科(研究院), 研究支援者 (80359851)
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| Co-Investigator(Renkei-kenkyūsha) |
WAZAWA Tetsuichi 東北大学, 大学院工学研究科, 研究支援者 (80359851)
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| Project Period (FY) |
2011-04-01 – 2014-03-31
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| Project Status |
Completed (Fiscal Year 2013)
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| Budget Amount *help |
¥19,500,000 (Direct Cost: ¥15,000,000、Indirect Cost: ¥4,500,000)
Fiscal Year 2013: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
Fiscal Year 2012: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
Fiscal Year 2011: ¥15,080,000 (Direct Cost: ¥11,600,000、Indirect Cost: ¥3,480,000)
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| Keywords | 局所粘度計測 / 蛍光偏光解消 / 回転相関時間 / ハイパーモバイル水 / アクチンフィラメント / 蛍光異方性 / 水和水 / 水和水の粘度 / ハイパーモバイル水が |
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| Research Abstract |
In this study, rotational correlation time Tc of fluorescent dye was measured to evaluate the local viscosity of the hydration region of protein surface.The Tc measured by this method was found to be in linear relation with the solvent viscosity. The CW-laser-based method revealed shorter Tc of Cy3 linked at Cys374 of actin in F-actin than around G-actin, which is consistent with the result by dielectric relaxation data.On the other hand,Tc (<1ns) of small fluorescent dye such as rhodamin 6G in water could not be measured by pulse-laser-based method without picosecond pulse width.We have designed a frequency-region fluorescent anisotropy spectroscopic method to measure Tc of small fluorescent dyes.
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Report
(4 results)
Research Products
(74 results)
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[Presentation] 誘電緩和分光法によるアルキルカルボン酸の水和特性
Author(s)
Yangtian Wang, Goerge Mogami, Tetsuichi Wazawa, Nobuyuki Morimoto, Makoto Suzuki
Organizer
第50回日本生物物理学会年会
Place of Presentation
名古屋大学
Related Report
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