• Search Research Projects
  • Search Researchers
  • How to Use
  1. Back to previous page

ADF-STEM Analysis of Magnetic Particles in Bio-system without Staining

Research Project

Project/Area Number 23310075
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Research Field Nanomaterials/Nanobioscience
Research InstitutionKyoto University

Principal Investigator

ISODA Seiji  京都大学, 物質-細胞統合システム拠点, 研究員 (00168288)

Co-Investigator(Kenkyū-buntansha) TAKANO Mikio  京都大学, 物質-細胞統合システム拠点 (70068138)
Project Period (FY) 2011-11-18 – 2014-03-31
Project Status Completed (Fiscal Year 2013)
Budget Amount *help
¥3,120,000 (Direct Cost: ¥2,400,000、Indirect Cost: ¥720,000)
Fiscal Year 2013: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2012: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Keywords無染色可視化 / 暗視野電子顕微鏡 / ソフトマテリアル / メゾスコピック系 / 可視化 / 走査型透過電子顕微鏡 / メゾスコピック / 磁性粒子 / 細胞・組織
Research Abstract

In the transfer mechanism of external particles into the biological system, the identification of pathways and final accumulation sites is essential for the understanding of the interaction of particles and biological systems. The aim of this study was to propose a methodology for high-resolution electron microscopic analysis of biological samples for realizing the direct spatial distribution analysis of the particle transfer.
In this study, a scanning transmission electron microscope dark field method was proposed for the analysis of a specimen mainly composed of light elements such as biological cells, by which we proposed a non-staining and also thick sample analysis. As a result, it was possible to analyze the localization of microparticles in cell samples introduced with magnetic particles by means of the non-staining electron microscopy,.

Report

(4 results)
  • 2013 Annual Research Report   Final Research Report ( PDF )
  • 2012 Annual Research Report
  • 2011 Annual Research Report
  • Research Products

    (35 results)

All 2013 2012 2011

All Journal Article (20 results) (of which Peer Reviewed: 20 results) Presentation (15 results) (of which Invited: 4 results)

  • [Journal Article] Quantitative Imaging of Tg in Block Copolymers by Low-Angle Annular Dark-Field Scanning Transmission Electron Microscopy2013

    • Author(s)
      Ryotaro Aso, Hiroki Kurata, Takeshi Namikoshi, Tamotsu Hashimoto, Shiao-Wei Kuo, Feng-Chih Chang, Hirokazu Hasegawa, Masahiko Tsujimoto, Mikio Takano, and Seiji Isoda
    • Journal Title

      Macromolecules

      Volume: 46(21) Pages: 8589-8595

    • Related Report
      2013 Annual Research Report 2013 Final Research Report
    • Peer Reviewed
  • [Journal Article] Integration of porous coordination polymers and gold nano-rods into core-shell mesoscopic composites towards light-induced molecular release2013

    • Author(s)
      Kira Khaletskaya, Julien Reboul, Mikhail Meilikhov, Masashi Nakahama, Stephane Diring, Masahiko Tsujimoto, Seiji Isoda, Franklin Kim, Ken-ichiro Kamei, Roland A. Fischer, Susumu Kitagawa and Shuhei Furukawa
    • Journal Title

      J. American Chemical Society

      Volume: 135(30) Pages: 10998-11005

    • Related Report
      2013 Final Research Report
    • Peer Reviewed
  • [Journal Article] Highly ordered alignment of polymer chains by host-guest cross-polymerization2013

    • Author(s)
      Gaetano Distefano, Takashi Uemura Hirohito Suzuki, Masahiko Tsujimoto, Seiji Isoda, Silvia Bracco, Angiolina Comotti, Piero Sozzani and Susumu Kitagawa
    • Journal Title

      Nature Chem.

      Volume: 5(4) Pages: 335-341

    • Related Report
      2013 Annual Research Report 2013 Final Research Report
    • Peer Reviewed
  • [Journal Article] Role of Adsorption Structures of Zn-Porphyrin on TiO2 in Dye-Sensitized Solar Cells Studied by Sum Frequency Generation Vibrational Spectroscopy and Ultrafast Spectroscopy2013

    • Author(s)
      Shen Ye, Arunkumar Kathiravan, Hironobu Hayashi, Yujin Tong, Yingyot Infahsaeng, Pavel Chabera, Torbjorn Pascher, Arkady P. Yartsev, Seiji Isoda, Hiroshi Imahori and Villy Sundstrom
    • Journal Title

      J. Physical Chemistry, C.

      Volume: 117 Pages: 6066-6080

    • Related Report
      2013 Final Research Report
    • Peer Reviewed
  • [Journal Article] Shape Memory Nanopores Induced in Coordination Frameworks by Crystal Downsizing2013

    • Author(s)
      Yoko Sakata, Shuhei Furukawa, Mio Kondo, Kenji Hirai, Nao Horike, Yohei Takashima, Hiromitsu Uehara, Nicolas Louvain, Mikhail Meilikhov, Takaaki Tsuruoka, Seiji Isoda, Wataru Kosaka, Osami Sakata, and Susumu Kitagawa
    • Journal Title

      Science

      Volume: 339(6116) Pages: 193-196

    • Related Report
      2013 Final Research Report
    • Peer Reviewed
  • [Journal Article] Anionic Complexes of MWCNT with Supergiant Cyanobacterial Polyanions2013

    • Author(s)
      Okajima, MK.; Maiko K.; Kumar, A.; Fujiwara, A.; Mitsumata, T.; Kaneko, D.; Ogawa, T.; Kurata, H.; Isoda, S.: Kaneko, T.
    • Journal Title

      Biopolymers

      Volume: 99(1) Pages: 1-9

    • Related Report
      2013 Final Research Report
    • Peer Reviewed
  • [Journal Article] Integration of porous coordination polymers and gold nano-rods into core-shell mesoscopic composites towards light-induced molecular release2013

    • Author(s)
      Kira Khaletskaya, Julien Reboul, Mikhail Meilikhov, Masashi Nakahama, Stéphane Diring, Masahiko Tsujimoto, Seiji Isoda, Franklin Kim, Ken-ichiro Kamei, Roland A. Fischer, Susumu Kitagawa and Shuhei Furukawa
    • Journal Title

      Journal of the American Chemical Society

      Volume: 135 Issue: 30 Pages: 10998-11005

    • DOI

      10.1021/ja403108x

    • Related Report
      2013 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Shape-Memory Nanopores Induced in Coordination Frameworks by Crystal Downsizing2013

    • Author(s)
      Yoko Sakata, Shuhei Furukawa, Kenji Hirai, Susuau Kitagawa, et al.
    • Journal Title

      Science

      Volume: 339 Issue: 6116 Pages: 193-196

    • DOI

      10.1126/science.1231451

    • Related Report
      2012 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Anionic Complexes of MWCNT with Supergiant Cyanobacterial Polyanions2013

    • Author(s)
      Okajima, MK.; Maiko K.; Kumar, A.; Fujiwara, A.; Mitsumata, T.; Kaneko, D.; Ogawa, T.; Kurata, H.; Isoda, S.: Kaneko, T.
    • Journal Title

      Biopolymers

      Volume: 99 Issue: 1 Pages: 109-109

    • DOI

      10.1002/bip.22129

    • Related Report
      2012 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Photodynamic and Photothermal Effects of Semiconducting and Metallic-Enriched Single-Walled Carbon Nanotubes2012

    • Author(s)
      Murakami, Tatsuya; Nakatsuji, Hirotaka; Inada, Mami; Matoba, Yoshinori; Umeyama, Tomokazu; Tsujimoto, Masahiko; Isoda, Seiji; Hashida, Mitsuru; Imahori, Hiroshi
    • Journal Title

      J. Am. Chem. Soc.

      Volume: 134(43) Pages: 17862-17865

    • Related Report
      2013 Final Research Report
    • Peer Reviewed
  • [Journal Article] Modular Design of Domain Assembly in Porous Coordination Polymer Crystals via Reactivity-Directed Crystallization Process2012

    • Author(s)
      Fukushima, Tomohiro; Horike, Satoshi; Kobayashi, Hirokazu; Tsujimoto, Masahiko; Isoda, Seiji; Foo, Maw Lin; Kubota, Yoshiki; Takata, Masaki; Kitagawa, Susumu
    • Journal Title

      J. Am. Chem. Soc.

      Volume: 134(32) Pages: 13341-13347

    • Related Report
      2013 Final Research Report
    • Peer Reviewed
  • [Journal Article] Nanotubes Assisted by Self-assembled Peptides Designed in Conjunction with β-Sheet Formation2012

    • Author(s)
      Yasuhiko Hashida, tomokazu Umeyama, Jyunya Mihara, Hiroshi Imahori, Masahimo Tsujimoto, Seiji Isoda and Mikio Takano
    • Journal Title

      J. Pharmaceutical Sciences

      Volume: 101(9) Pages: 3398-3412

    • Related Report
      2013 Final Research Report
    • Peer Reviewed
  • [Journal Article] Highly photoconducting .-Stacked Polymer accommodated in coordination nanochannels2012

    • Author(s)
      Takashi Uemura, Noriyuki Uchida, Atsushi Asano, Akinori Saeki, Shu Seki, Masahiko Tsujimoto, Seiji Isoda, and Susumu Kitagawa
    • Journal Title

      JACS

      Volume: 134 (20) Pages: 8360-8363

    • Related Report
      2013 Final Research Report
    • Peer Reviewed
  • [Journal Article] Guest-to-Host Structural Transmission for Stimuli-Responsive Adsorption Property2012

    • Author(s)
      Nobuhiro Yanai, Takashi Uemura, Masafumi Inoue, Ryotaro Matsuda, Tomohiro Fukushima, Masahiko Tsujimoto, Seiji Isoda, and Susumu Kitagawa
    • Journal Title

      JACS. Communication

      Volume: 134(10) Pages: 4501-4504

    • Related Report
      2013 Final Research Report
    • Peer Reviewed
  • [Journal Article] A Photoconductive, Thiophene-Fullerene Double-Cable Polymer, Nanorod Device2012

    • Author(s)
      Hiroshi Imahori, Shinji Kitaura, Aiko Kira, Hironobu Hayashi, Masayuki Nishi, Kazuyuki Hirao, Seiji Isoda, Masahiko Tsujimoto, Mikio Takano, Zhang Zhe, Yuji Miyato, Kei Noda, Kazumi Matsushige, Kati Stranius, Nikolai V. Tkachenko, Helge Lemmetyinen, Lidong Qin, Sarah J. Hurst and Chad A. Mirkin
    • Journal Title

      J. Phys. Chem., Lett.

      Volume: 3 Pages: 478-481

    • Related Report
      2013 Final Research Report
    • Peer Reviewed
  • [Journal Article] Development of a Novel Composite Material with Carbon Nanotubes Assisted by Self-assembled Peptides Designed in Conjunction with β-Sheet Formation2012

    • Author(s)
      Y. Hashida, T. Umeyama, J. Mihara, H. Imahori, M. Tsujimoto, S. Isoda and M. Takano
    • Journal Title

      Journal of Pharmaceutical Sciences

      Volume: 101 Issue: 27 Pages: 3398-3412

    • DOI

      10.1002/anie.201200474

    • Related Report
      2012 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Development of a Novel Composite Material with Carbon Nanotubes Assisted by Self-assembled Peptides Designed in Conjunction with β-Sheet Formation2012

    • Author(s)
      Yasuhiko Hashida, Tomokazu Umeyama, Jyunya Mihara, Hiroshi Imahori, Masahimo Tsujimoto, Seiji Isoda, Mikio Takano
    • Journal Title

      Journal of Pharmaceutical Sciences

      Volume: (印刷中)

    • Related Report
      2011 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Effects of Fullerene Encapsulation on Photodynamics of Porphyrin-Linked Single-Walled Carbon Nanotubes2011

    • Author(s)
      Tomokazu Umeyama, Junya Mihara, Hironobu Hayashi, Naoki Kadota, Vladimir Chukharev, Nikolai V. Tkachenko, Helge Lemmtyinen, Kaname Yoshida, Seiji Isoda and Hirohi Imahori
    • Journal Title

      Chem.Commun

      Volume: 47(42) Pages: 11781-11783

    • Related Report
      2013 Final Research Report
    • Peer Reviewed
  • [Journal Article] Photoinduced Charge Carrier Dynamics of Zn-Porphyrin-TiO2 Electrodes . The Key Role of Charge Recombination for Solar Cell Performance2011

    • Author(s)
      Imahori, Hiroshi; Kang, Soonchul; Hayashi, Hironobu; Haruta, Mitsutaka; Kurata, Hiroki; Isoda, Seiji; Canton, Sophie; Infahsaeng, Yingyot; Kathiravan, Arunkumar; Pascher, Torbjorn; Chabera, Pavel; Yartsev, Arkady and Sundstrom, Villy
    • Journal Title

      The Journal of Physical Chemistry, A

      Volume: 115(16) Pages: 3679-3690

    • Related Report
      2013 Final Research Report
    • Peer Reviewed
  • [Journal Article] Photophysics and Photoelectrochemical Properties of Nanohybrids Consisting of Fullerene-encapsulated Single-Walled Carbon Nanotubes and Poly-(3-hexylthiophene)2011

    • Author(s)
      Noriyasu Tezuka, Tomokazu Umeyama, Yoshihiro Matano, Tetsuya Ogawa, Seiji Isoda, Kati Stranius, Vladimir Chukharev, Nikolai V. Tkachenkod, Helge Lemmetyinen and Hiroshi Imahori
    • Journal Title

      Energy & Environmental Science

      Volume: 4 Pages: 741-750

    • Related Report
      2013 Final Research Report
    • Peer Reviewed
  • [Presentation] Surface Modification of 1D Nanostructures2013

    • Author(s)
      Seiji Isoda
    • Organizer
      The 5th Symposium of Promotion Center for Global Materials Research (PCGMR)
    • Place of Presentation
      Tainan, Taiwan
    • Related Report
      2013 Final Research Report
  • [Presentation] Quantitative Imaging of Micro-phases in Copolymers by LAADF-STEM2013

    • Author(s)
      Ryotaro Aso, Hiroki Kurata, and Seiji Isoda
    • Organizer
      The 13th Pacific Polymer Conference (PPC 2013)
    • Place of Presentation
      Kaohsiung, Taiwan
    • Related Report
      2013 Final Research Report
  • [Presentation] Photodynamic and Photothermal Effects of Semiconducting and Metallic-Enriched Single-Walled Carbon Nanotubes for Cancer Cell Killing2013

    • Author(s)
      Hirotaka Nakatsuji, Tatsuya Murakami, Mami Inada, Yoshinori Matoba, Tomokazu Umeyama, Masahiko Tsujimoto, Seiji Isoda, Mitsuru Hashida and Hiroshi Imahori
    • Organizer
      223rd ECS Meeting
    • Place of Presentation
      Toronto, Ontario, Canada
    • Related Report
      2013 Final Research Report
  • [Presentation] Molecular imaging by electron microscopy2013

    • Author(s)
      S. Isoda and H.Kurata
    • Organizer
      The 30-th Annual Conference of Microscopy Society of Thailand
    • Place of Presentation
      Chanthaburi, Thailand
    • Related Report
      2013 Final Research Report
  • [Presentation] Surface Modification of 1D Nanostructures2013

    • Author(s)
      Seiji Isoda
    • Organizer
      The 5th Symposium of Promotion Center for Global Materials Research
    • Place of Presentation
      Tainan, Taiwan
    • Related Report
      2013 Annual Research Report
    • Invited
  • [Presentation] Quantitative Imaging of Micro-phases in Copolymers by LAADF-STEM2013

    • Author(s)
      Ryotaro Aso, Hiroki Kurata, and Seiji Isoda
    • Organizer
      The 13th Pacific Polymer Conference
    • Place of Presentation
      Kaohsiung, Taiwan
    • Related Report
      2013 Annual Research Report
    • Invited
  • [Presentation] Photodynamic and Photothermal Effects of Semiconducting and Metallic-Enriched Single-Walled Carbon Nanotubes for Cancer Cell Killing2013

    • Author(s)
      Hirotaka Nakatsuji, Tatsuya Murakami, Mami Inada, Yoshinori Matoba, Tomokazu Umeyama, Masahiko Tsujimoto, Seiji Isoda, Mitsuru Hashida and Hiroshi Imahori
    • Organizer
      The 223rd ECS Meeting
    • Place of Presentation
      Toronto, Canada
    • Related Report
      2013 Annual Research Report
  • [Presentation] Molecular imaging by electron microscopy2013

    • Author(s)
      S. Isoda and H.Kurata
    • Organizer
      The 30-th Annual Conference of Microscopy Society of Thailand
    • Place of Presentation
      Maneechan Resort, Chanthaburi, Thailand
    • Related Report
      2012 Annual Research Report
    • Invited
  • [Presentation] Local Electronic Structure Analysis for Ca(Sr)FeO2.5 by Site-resolved Energy-loss Near-edge Structures2012

    • Author(s)
      M.Haruta, H.Kurata, S.Isoda
    • Organizer
      The 10th Asia-Pacific Microscopy Conference
    • Place of Presentation
      Perth, Australia(招待講演)
    • Year and Date
      2012-02-07
    • Related Report
      2011 Annual Research Report
  • [Presentation] Atomic resolution imaging of organic crystals by STEM2012

    • Author(s)
      Mitsutaka Haruta, Seiji Isoda and Hiroki Kurata
    • Organizer
      The 9-th Polish-Japanese-Joint Seminar on Micro and Nano Analysis
    • Place of Presentation
      Sieniawa, Poland
    • Related Report
      2013 Final Research Report
  • [Presentation] ADF-STEM Analysis for Soft Materials2012

    • Author(s)
      Ryotaro Aso, Masahiko Tsujimoto, Hiroki Kurata, and Seiji Isoda
    • Organizer
      The 9-th Polish-Japanese-Joint Seminar on Micro and Nano Analysis
    • Place of Presentation
      Sieniawa, Poland
    • Related Report
      2013 Final Research Report
  • [Presentation] ADF-STEM Analysis for Soft Materials2012

    • Author(s)
      R. Aso, M. Tsujimoto, H. Kurata, and S. Isoda
    • Organizer
      The 9-th Polish-Japanese-Joint Seminar on Micro and Nano Analysis
    • Place of Presentation
      Sieniawa Hotel, Sieniawa, Poland
    • Related Report
      2012 Annual Research Report
    • Invited
  • [Presentation] ADF-STEM Imaging of Block Copolymers without Staining2011

    • Author(s)
      Ryotaro Aso, Mitsutaka Haruta, Takeshi Namikoshi, Tamotsu Hashimoto, Hiroki Kurata and Seiji Isoda
    • Organizer
      The International Union of Materials Research Societies -International Conference in Asia (IUMRS-ICA)
    • Place of Presentation
      Taipei, Taiwan
    • Related Report
      2013 Final Research Report
  • [Presentation] Environmentally-friendly Materials as Photosensitizers for Dye-sensitized Solar Cell2011

    • Author(s)
      H. Takano, S. Isoda, M. Tsujimoto, M. Adachi, T. Kiyomura, T. Nemoto and H. Kurata
    • Organizer
      The International Union of Materials Research Societies -International Conference in Asia (IUMRS-ICA)
    • Place of Presentation
      Taipei, Taiwan
    • Related Report
      2013 Final Research Report
  • [Presentation] Mesocrystalline Structure of Copper Oxalate Nanocrystals2011

    • Author(s)
      Jean-Christophe Valmalette, Tetsuya Ogawa, Julien Romann and Seiji Isoda
    • Organizer
      E-MRS conference
    • Place of Presentation
      Nice, France
    • Related Report
      2013 Final Research Report

URL: 

Published: 2013-05-15   Modified: 2019-07-29  

Information User Guide FAQ News Terms of Use Attribution of KAKENHI

Powered by NII kakenhi