ADF-STEM Analysis of Magnetic Particles in Bio-system without Staining

• Project/Area Number: 23310075
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Nanomaterials/Nanobioscience
• Research Institution: Kyoto University
• Principal Investigator: ISODA Seiji 京都大学, 物質-細胞統合システム拠点, 研究員 (00168288)
• Co-Investigator(Kenkyū-buntansha): TAKANO Mikio 京都大学, 物質-細胞統合システム拠点 (70068138)
• Project Period (FY): 2011-11-18 – 2014-03-31
• Project Status: Completed (Fiscal Year 2013)
• Budget Amount: ¥3,120,000 (Direct Cost: ¥2,400,000、Indirect Cost: ¥720,000); Fiscal Year 2013: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000); Fiscal Year 2012: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
• Keywords: 無染色可視化 / 暗視野電子顕微鏡 / ソフトマテリアル / メゾスコピック系 / 可視化 / 走査型透過電子顕微鏡 / メゾスコピック / 磁性粒子 / 細胞・組織

Research Abstract

In the transfer mechanism of external particles into the biological system, the identification of pathways and final accumulation sites is essential for the understanding of the interaction of particles and biological systems. The aim of this study was to propose a methodology for high-resolution electron microscopic analysis of biological samples for realizing the direct spatial distribution analysis of the particle transfer.
In this study, a scanning transmission electron microscope dark field method was proposed for the analysis of a specimen mainly composed of light elements such as biological cells, by which we proposed a non-staining and also thick sample analysis. As a result, it was possible to analyze the localization of microparticles in cell samples introduced with magnetic particles by means of the non-staining electron microscopy,.

Research Products

• [Journal Article] Quantitative Imaging of Tg in Block Copolymers by Low-Angle Annular Dark-Field Scanning Transmission Electron Microscopy (2013)
  • Author(s): Ryotaro Aso, Hiroki Kurata, Takeshi Namikoshi, Tamotsu Hashimoto, Shiao-Wei Kuo, Feng-Chih Chang, Hirokazu Hasegawa, Masahiko Tsujimoto, Mikio Takano, and Seiji Isoda
  • Journal Title: Macromolecules Volume: 46(21) Pages: 8589-8595
  • Peer Reviewed
• [Journal Article] Integration of porous coordination polymers and gold nano-rods into core-shell mesoscopic composites towards light-induced molecular release (2013)
  • Author(s): Kira Khaletskaya, Julien Reboul, Mikhail Meilikhov, Masashi Nakahama, Stephane Diring, Masahiko Tsujimoto, Seiji Isoda, Franklin Kim, Ken-ichiro Kamei, Roland A. Fischer, Susumu Kitagawa and Shuhei Furukawa
  • Journal Title: J. American Chemical Society Volume: 135(30) Pages: 10998-11005
  • Peer Reviewed
• [Journal Article] Highly ordered alignment of polymer chains by host-guest cross-polymerization (2013)
  • Author(s): Gaetano Distefano, Takashi Uemura Hirohito Suzuki, Masahiko Tsujimoto, Seiji Isoda, Silvia Bracco, Angiolina Comotti, Piero Sozzani and Susumu Kitagawa
  • Journal Title: Nature Chem. Volume: 5(4) Pages: 335-341
  • Peer Reviewed
• [Journal Article] Role of Adsorption Structures of Zn-Porphyrin on TiO2 in Dye-Sensitized Solar Cells Studied by Sum Frequency Generation Vibrational Spectroscopy and Ultrafast Spectroscopy (2013)
  • Author(s): Shen Ye, Arunkumar Kathiravan, Hironobu Hayashi, Yujin Tong, Yingyot Infahsaeng, Pavel Chabera, Torbjorn Pascher, Arkady P. Yartsev, Seiji Isoda, Hiroshi Imahori and Villy Sundstrom
  • Journal Title: J. Physical Chemistry, C. Volume: 117 Pages: 6066-6080
  • Peer Reviewed
• [Journal Article] Shape Memory Nanopores Induced in Coordination Frameworks by Crystal Downsizing (2013)
  • Author(s): Yoko Sakata, Shuhei Furukawa, Mio Kondo, Kenji Hirai, Nao Horike, Yohei Takashima, Hiromitsu Uehara, Nicolas Louvain, Mikhail Meilikhov, Takaaki Tsuruoka, Seiji Isoda, Wataru Kosaka, Osami Sakata, and Susumu Kitagawa
  • Journal Title: Science Volume: 339(6116) Pages: 193-196
  • Peer Reviewed
• [Journal Article] Anionic Complexes of MWCNT with Supergiant Cyanobacterial Polyanions (2013)
  • Author(s): Okajima, MK.; Maiko K.; Kumar, A.; Fujiwara, A.; Mitsumata, T.; Kaneko, D.; Ogawa, T.; Kurata, H.; Isoda, S.: Kaneko, T.
  • Journal Title: Biopolymers Volume: 99(1) Pages: 1-9
  • Peer Reviewed
• [Journal Article] Integration of porous coordination polymers and gold nano-rods into core-shell mesoscopic composites towards light-induced molecular release (2013)
  • Author(s): Kira Khaletskaya, Julien Reboul, Mikhail Meilikhov, Masashi Nakahama, Stéphane Diring, Masahiko Tsujimoto, Seiji Isoda, Franklin Kim, Ken-ichiro Kamei, Roland A. Fischer, Susumu Kitagawa and Shuhei Furukawa
  • Journal Title: Journal of the American Chemical Society Volume: 135 Issue: 30 Pages: 10998-11005
  • DOI: 10.1021/ja403108x
  • Peer Reviewed
• [Journal Article] Shape-Memory Nanopores Induced in Coordination Frameworks by Crystal Downsizing (2013)
  • Author(s): Yoko Sakata, Shuhei Furukawa, Kenji Hirai, Susuau Kitagawa, et al.
  • Journal Title: Science Volume: 339 Issue: 6116 Pages: 193-196
  • DOI: 10.1126/science.1231451
  • Peer Reviewed
• [Journal Article] Anionic Complexes of MWCNT with Supergiant Cyanobacterial Polyanions (2013)
  • Author(s): Okajima, MK.; Maiko K.; Kumar, A.; Fujiwara, A.; Mitsumata, T.; Kaneko, D.; Ogawa, T.; Kurata, H.; Isoda, S.: Kaneko, T.
  • Journal Title: Biopolymers Volume: 99 Issue: 1 Pages: 109-109
  • DOI: 10.1002/bip.22129
  • Peer Reviewed
• [Journal Article] Photodynamic and Photothermal Effects of Semiconducting and Metallic-Enriched Single-Walled Carbon Nanotubes (2012)
  • Author(s): Murakami, Tatsuya; Nakatsuji, Hirotaka; Inada, Mami; Matoba, Yoshinori; Umeyama, Tomokazu; Tsujimoto, Masahiko; Isoda, Seiji; Hashida, Mitsuru; Imahori, Hiroshi
  • Journal Title: J. Am. Chem. Soc. Volume: 134(43) Pages: 17862-17865
  • Peer Reviewed
• [Journal Article] Modular Design of Domain Assembly in Porous Coordination Polymer Crystals via Reactivity-Directed Crystallization Process (2012)
  • Author(s): Fukushima, Tomohiro; Horike, Satoshi; Kobayashi, Hirokazu; Tsujimoto, Masahiko; Isoda, Seiji; Foo, Maw Lin; Kubota, Yoshiki; Takata, Masaki; Kitagawa, Susumu
  • Journal Title: J. Am. Chem. Soc. Volume: 134(32) Pages: 13341-13347
  • Peer Reviewed
• [Journal Article] Nanotubes Assisted by Self-assembled Peptides Designed in Conjunction with β-Sheet Formation (2012)
  • Author(s): Yasuhiko Hashida, tomokazu Umeyama, Jyunya Mihara, Hiroshi Imahori, Masahimo Tsujimoto, Seiji Isoda and Mikio Takano
  • Journal Title: J. Pharmaceutical Sciences Volume: 101(9) Pages: 3398-3412
  • Peer Reviewed
• [Journal Article] Highly photoconducting .-Stacked Polymer accommodated in coordination nanochannels (2012)
  • Author(s): Takashi Uemura, Noriyuki Uchida, Atsushi Asano, Akinori Saeki, Shu Seki, Masahiko Tsujimoto, Seiji Isoda, and Susumu Kitagawa
  • Journal Title: JACS Volume: 134 (20) Pages: 8360-8363
  • Peer Reviewed
• [Journal Article] Guest-to-Host Structural Transmission for Stimuli-Responsive Adsorption Property (2012)
  • Author(s): Nobuhiro Yanai, Takashi Uemura, Masafumi Inoue, Ryotaro Matsuda, Tomohiro Fukushima, Masahiko Tsujimoto, Seiji Isoda, and Susumu Kitagawa
  • Journal Title: JACS. Communication Volume: 134(10) Pages: 4501-4504
  • Peer Reviewed
• [Journal Article] A Photoconductive, Thiophene-Fullerene Double-Cable Polymer, Nanorod Device (2012)
  • Author(s): Hiroshi Imahori, Shinji Kitaura, Aiko Kira, Hironobu Hayashi, Masayuki Nishi, Kazuyuki Hirao, Seiji Isoda, Masahiko Tsujimoto, Mikio Takano, Zhang Zhe, Yuji Miyato, Kei Noda, Kazumi Matsushige, Kati Stranius, Nikolai V. Tkachenko, Helge Lemmetyinen, Lidong Qin, Sarah J. Hurst and Chad A. Mirkin
  • Journal Title: J. Phys. Chem., Lett. Volume: 3 Pages: 478-481
  • Peer Reviewed
• [Journal Article] Development of a Novel Composite Material with Carbon Nanotubes Assisted by Self-assembled Peptides Designed in Conjunction with β-Sheet Formation (2012)
  • Author(s): Y. Hashida, T. Umeyama, J. Mihara, H. Imahori, M. Tsujimoto, S. Isoda and M. Takano
  • Journal Title: Journal of Pharmaceutical Sciences Volume: 101 Issue: 27 Pages: 3398-3412
  • DOI: 10.1002/anie.201200474
  • Peer Reviewed
• [Journal Article] Development of a Novel Composite Material with Carbon Nanotubes Assisted by Self-assembled Peptides Designed in Conjunction with β-Sheet Formation (2012)
  • Author(s): Yasuhiko Hashida, Tomokazu Umeyama, Jyunya Mihara, Hiroshi Imahori, Masahimo Tsujimoto, Seiji Isoda, Mikio Takano
  • Journal Title: Journal of Pharmaceutical Sciences Volume: (印刷中)
  • Peer Reviewed
• [Journal Article] Effects of Fullerene Encapsulation on Photodynamics of Porphyrin-Linked Single-Walled Carbon Nanotubes (2011)
  • Author(s): Tomokazu Umeyama, Junya Mihara, Hironobu Hayashi, Naoki Kadota, Vladimir Chukharev, Nikolai V. Tkachenko, Helge Lemmtyinen, Kaname Yoshida, Seiji Isoda and Hirohi Imahori
  • Journal Title: Chem.Commun Volume: 47(42) Pages: 11781-11783
  • Peer Reviewed
• [Journal Article] Photoinduced Charge Carrier Dynamics of Zn-Porphyrin-TiO2 Electrodes . The Key Role of Charge Recombination for Solar Cell Performance (2011)
  • Author(s): Imahori, Hiroshi; Kang, Soonchul; Hayashi, Hironobu; Haruta, Mitsutaka; Kurata, Hiroki; Isoda, Seiji; Canton, Sophie; Infahsaeng, Yingyot; Kathiravan, Arunkumar; Pascher, Torbjorn; Chabera, Pavel; Yartsev, Arkady and Sundstrom, Villy
  • Journal Title: The Journal of Physical Chemistry, A Volume: 115(16) Pages: 3679-3690
  • Peer Reviewed
• [Journal Article] Photophysics and Photoelectrochemical Properties of Nanohybrids Consisting of Fullerene-encapsulated Single-Walled Carbon Nanotubes and Poly-(3-hexylthiophene) (2011)
  • Author(s): Noriyasu Tezuka, Tomokazu Umeyama, Yoshihiro Matano, Tetsuya Ogawa, Seiji Isoda, Kati Stranius, Vladimir Chukharev, Nikolai V. Tkachenkod, Helge Lemmetyinen and Hiroshi Imahori
  • Journal Title: Energy & Environmental Science Volume: 4 Pages: 741-750
  • Peer Reviewed
• [Presentation] Surface Modification of 1D Nanostructures (2013)
  • Author(s): Seiji Isoda
  • Organizer: The 5th Symposium of Promotion Center for Global Materials Research (PCGMR)
  • Place of Presentation: Tainan, Taiwan
• [Presentation] Quantitative Imaging of Micro-phases in Copolymers by LAADF-STEM (2013)
  • Author(s): Ryotaro Aso, Hiroki Kurata, and Seiji Isoda
  • Organizer: The 13th Pacific Polymer Conference (PPC 2013)
  • Place of Presentation: Kaohsiung, Taiwan
• [Presentation] Photodynamic and Photothermal Effects of Semiconducting and Metallic-Enriched Single-Walled Carbon Nanotubes for Cancer Cell Killing (2013)
  • Author(s): Hirotaka Nakatsuji, Tatsuya Murakami, Mami Inada, Yoshinori Matoba, Tomokazu Umeyama, Masahiko Tsujimoto, Seiji Isoda, Mitsuru Hashida and Hiroshi Imahori
  • Organizer: 223rd ECS Meeting
  • Place of Presentation: Toronto, Ontario, Canada
• [Presentation] Molecular imaging by electron microscopy (2013)
  • Author(s): S. Isoda and H.Kurata
  • Organizer: The 30-th Annual Conference of Microscopy Society of Thailand
  • Place of Presentation: Chanthaburi, Thailand
• [Presentation] Surface Modification of 1D Nanostructures (2013)
  • Author(s): Seiji Isoda
  • Organizer: The 5th Symposium of Promotion Center for Global Materials Research
  • Place of Presentation: Tainan, Taiwan
  • Invited
• [Presentation] Quantitative Imaging of Micro-phases in Copolymers by LAADF-STEM (2013)
  • Author(s): Ryotaro Aso, Hiroki Kurata, and Seiji Isoda
  • Organizer: The 13th Pacific Polymer Conference
  • Place of Presentation: Kaohsiung, Taiwan
  • Invited
• [Presentation] Photodynamic and Photothermal Effects of Semiconducting and Metallic-Enriched Single-Walled Carbon Nanotubes for Cancer Cell Killing (2013)
  • Author(s): Hirotaka Nakatsuji, Tatsuya Murakami, Mami Inada, Yoshinori Matoba, Tomokazu Umeyama, Masahiko Tsujimoto, Seiji Isoda, Mitsuru Hashida and Hiroshi Imahori
  • Organizer: The 223rd ECS Meeting
  • Place of Presentation: Toronto, Canada
• [Presentation] Molecular imaging by electron microscopy (2013)
  • Author(s): S. Isoda and H.Kurata
  • Organizer: The 30-th Annual Conference of Microscopy Society of Thailand
  • Place of Presentation: Maneechan Resort, Chanthaburi, Thailand
  • Invited
• [Presentation] Local Electronic Structure Analysis for Ca(Sr)FeO2.5 by Site-resolved Energy-loss Near-edge Structures (2012)
  • Author(s): M.Haruta, H.Kurata, S.Isoda
  • Organizer: The 10th Asia-Pacific Microscopy Conference
  • Place of Presentation: Perth, Australia(招待講演)
  • Year and Date: 2012-02-07
• [Presentation] Atomic resolution imaging of organic crystals by STEM (2012)
  • Author(s): Mitsutaka Haruta, Seiji Isoda and Hiroki Kurata
  • Organizer: The 9-th Polish-Japanese-Joint Seminar on Micro and Nano Analysis
  • Place of Presentation: Sieniawa, Poland
• [Presentation] ADF-STEM Analysis for Soft Materials (2012)
  • Author(s): Ryotaro Aso, Masahiko Tsujimoto, Hiroki Kurata, and Seiji Isoda
  • Organizer: The 9-th Polish-Japanese-Joint Seminar on Micro and Nano Analysis
  • Place of Presentation: Sieniawa, Poland
• [Presentation] ADF-STEM Analysis for Soft Materials (2012)
  • Author(s): R. Aso, M. Tsujimoto, H. Kurata, and S. Isoda
  • Organizer: The 9-th Polish-Japanese-Joint Seminar on Micro and Nano Analysis
  • Place of Presentation: Sieniawa Hotel, Sieniawa, Poland
  • Invited
• [Presentation] ADF-STEM Imaging of Block Copolymers without Staining (2011)
  • Author(s): Ryotaro Aso, Mitsutaka Haruta, Takeshi Namikoshi, Tamotsu Hashimoto, Hiroki Kurata and Seiji Isoda
  • Organizer: The International Union of Materials Research Societies -International Conference in Asia (IUMRS-ICA)
  • Place of Presentation: Taipei, Taiwan
• [Presentation] Environmentally-friendly Materials as Photosensitizers for Dye-sensitized Solar Cell (2011)
  • Author(s): H. Takano, S. Isoda, M. Tsujimoto, M. Adachi, T. Kiyomura, T. Nemoto and H. Kurata
  • Organizer: The International Union of Materials Research Societies -International Conference in Asia (IUMRS-ICA)
  • Place of Presentation: Taipei, Taiwan
• [Presentation] Mesocrystalline Structure of Copper Oxalate Nanocrystals (2011)
  • Author(s): Jean-Christophe Valmalette, Tetsuya Ogawa, Julien Romann and Seiji Isoda
  • Organizer: E-MRS conference
  • Place of Presentation: Nice, France