| Budget Amount *help |
¥9,880,000 (Direct Cost: ¥7,600,000、Indirect Cost: ¥2,280,000)
Fiscal Year 2013: ¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000)
Fiscal Year 2012: ¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000)
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| Research Abstract |
The nucleoid protein, H-NS, binds hundreds of regions on the E. coli genome and consequently silences the expression of many genes. Interestingly, many of the genes silenced by H-NS are horizontally acquired, some that are integrated into host transcriptional regulatory networks contribute to stress response, virulence and pathogenicity of the host E. coli strains. Here, we report a comparative evaluation of H-NS binding profiles, determined using the ChAP-seq method, to conserved common segments in the genome sequences of three E.coli strains, K-12, SE11, and SE15. We observed that the H-NS binding profiles are highly conserved in common segments among the strains, and sequence diversity within these regions in the different strains does not affect H-NS interactions. Rather, sequence-diverse regions of the common segments in the E. coli genome tend to bind H-NS, and the encoded genes in these regions seem to play important roles in growth during intestinal environment.
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