Roles of type II DNA topoisomerase in the interaction of distant genomic sites
| Project/Area Number |
23310133
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Genome biology
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| Research Institution | Okayama University |
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Principal Investigator |
TSUTSUI KEN 岡山大学, 医歯(薬)学総合研究科, 名誉教授 (70108158)
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| Co-Investigator(Kenkyū-buntansha) |
MIYAJI Mary 岡山大学, 大学院・医歯薬学総合研究科, 助教 (50349255)
HOSOYA Osamu 岡山大学, 大学院・医歯薬学総合研究科, 助教 (90304310)
SANO Kuniaki 岡山大学, 大学院・医歯薬学総合研究科, 助教 (00294405)
TSUTSUI Kimiko 岡山大学, 大学院・医歯薬学総合研究科, 助教 (70144748)
KUWANO Ryozo 新潟大学, 脳研究所, 教授 (20111734)
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| Project Period (FY) |
2011-04-01 – 2014-03-31
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| Project Status |
Completed (Fiscal Year 2013)
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| Budget Amount *help |
¥20,410,000 (Direct Cost: ¥15,700,000、Indirect Cost: ¥4,710,000)
Fiscal Year 2013: ¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000)
Fiscal Year 2012: ¥8,320,000 (Direct Cost: ¥6,400,000、Indirect Cost: ¥1,920,000)
Fiscal Year 2011: ¥7,540,000 (Direct Cost: ¥5,800,000、Indirect Cost: ¥1,740,000)
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| Keywords | 遺伝子 / ゲノム / 酵素 / 発現制御 / 発生・分化 / 発生 / 分化 |
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| Research Abstract |
We analyzed the characteristic gene regulation mechanism in terminally differentiating neuronal cells by identifying the genomic region targeted by DNA topoisomerase IIbeta (topo IIb) and its partner protein SP120/hnRNP U through massive sequencing on new generation sequencers. Results are summarized as follows: 1) Topo IIb regulates the transcription of groups of genes in both directions (up- and down-regulation). 2) Genes controlled by topo IIb are positioned non-randomly on the genome. 3) Analysis of the topo IIb action sites (toposites) suggested that the toposite enriched in intergenic regions (termed Ts3) has an important role in the gene expression of terminal differentiation. 4) The SP120-binding sites overlapping with Ts3 toposites appear to be the target of topo IIb-SP120 complex, which was also demonstrated by cytological methods as nuclear colocalization spots. 5) Ts3 toposites represent the topo IIb-mediated distant interaction sites involved.
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Report
(4 results)
Research Products
(39 results)
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[Journal Article] The CENP-O complex requirement varies among different cell types.2014
Author(s)
Kagawa, N., Hori, T., Hoki, Y., Hosoya, O., Tsutsui, K., Saga, Y., Sado, T., and *Fukagawa, T.
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Journal Title
Chrom. Research
Volume: in press
Issue: 3
Pages: 293-303
DOI
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Peer Reviewed / Open Access
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[Presentation] Induced Expression of Neuroal Genes in Terminal Differentiation: Essential Roles of DNA Topoisomerase IIbeta.2012
Author(s)
Tsutsui, K.M., Miyaji, M., Sano, K., Furuta, R., Tsutsui, K.
Organizer
BIT’s 3rd Annual World Congress of NeuroTalk-2012.
Place of Presentation
Beijing International Convention Center, Beijing, China
Related Report
Invited
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[Presentation] DNA Topoisomerase IIbeta Mediates Distal Genomic Interactions in Terminal differentiation of Neurons.2012
Author(s)
Furuta, R., Miyaji, M., Sano, K., Tsutsui, K.M., Tsutsui, K.
Organizer
BIT’s 3rd Annual World Congress of NeuroTalk-2012.
Place of Presentation
Beijing International Convention Center, Beijing, China
Related Report
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[Book] Catalase gene mutant mice; Acatalasemic and hypocatalasemin mice. In “Advances in Medicine and Biology”2013
Author(s)
Wang, D.H., Ogino, K., Tsutsui, K., Sano, K., Masuoka, N., Sugiyama, H., Miyazaki, M., Fujita, H., Taketa, K., Ogata, M.
Publisher
NOVA Science publishers, Inc., New York
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