Lineage potential regulated by high order chromatin structure
| Project/Area Number |
23310134
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Genome biology
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| Research Institution | Kyushu University |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
OKADA Seiji 九州大学, 医学(系)研究科(研究院), 准教授 (30448435)
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| Project Period (FY) |
2011-04-01 – 2014-03-31
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| Project Status |
Completed (Fiscal Year 2013)
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| Budget Amount *help |
¥20,540,000 (Direct Cost: ¥15,800,000、Indirect Cost: ¥4,740,000)
Fiscal Year 2013: ¥5,980,000 (Direct Cost: ¥4,600,000、Indirect Cost: ¥1,380,000)
Fiscal Year 2012: ¥5,980,000 (Direct Cost: ¥4,600,000、Indirect Cost: ¥1,380,000)
Fiscal Year 2011: ¥8,580,000 (Direct Cost: ¥6,600,000、Indirect Cost: ¥1,980,000)
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| Keywords | クロマチン / 骨格筋分化 / 次世代シークエンサー / ジーンクラスタリング |
|---|
| Research Abstract |
Lineage potential is triggered by lineage-specific transcription factor expression in association with structural chromatin changes. Histone H3.3 variant is a critical chromatin component that regulates lineage potential, but the function of each H3 variant remains unclear. Here, we found that forced incorporation of H3.1 (canonical H3 variant) into lineage-specific genes diminished trimethylation on H3K4 (H3K4me3) and increased trimethylation on H3K27 (H3K27me3), resulting in loss of lineage potential. In mouse embryos, bivalent modifications of H3K4me3 and H3K27me3 were equivalently formed on the H3.3-incorporated region before embryonic skeletal muscle differentiation at myogenic loci. These results suggest that lineage potential is established through selective H3.3 incorporation into chromatin and is defined by a quantitative balance among histone modifications.
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Report
(4 results)
Research Products
(98 results)
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[Journal Article] β-Catenin signaling regulates Foxa2 expression during endometrial hyperplasia formation2013
Author(s)
Villacorte M, Suzuki K, Hirasawa A, Ohkawa Y, Suyama M, Maruyama T, Aoki D, Ogino Y, Miyagawa S, Terabayashi T, Tomooka Y, Nakagata N, Yamada G
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Journal Title
Oncogene
Volume: 32
Issue: 29
Pages: 3477-3482
DOI
Related Report
Peer Reviewed
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[Journal Article] Chd2 interacts with H3.3 to determine myogenic cell fate2012
Author(s)
Harada A, Okada S, Konno D, Odawara J, Yoshimi T, Yoshimura S, Kumamaru H, Saiwai H, Tsubota T, Kurumizaka H, Akashi K, Tachibana T, Imbalzano AN, Ohkawa Y
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Journal Title
EMBO J
Volume: 31
Issue: 13
Pages: 2994-3007
DOI
Related Report
Peer Reviewed
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[Presentation] RNA polymerase IIのC末端ドメイン構造の非リン酸化修飾の網羅的解読2013
Author(s)
小田原 淳, 前原 一満, 成相 直樹,原田 哲仁,林 正康,植野 和子,吉見 智彦,立花 太郎, 長崎 正朗, 赤司 浩一,大川 恭行
Organizer
第36回日本分子生物学会年会
Place of Presentation
神戸ポートアイランド（兵庫）
Related Report
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[Presentation] 選択的なヒストンバリアントH3.3の取り込みによる造血細胞分化運命決定機構の解明
Author(s)
小田原 淳, 植野 和子, 成相 直樹, 林 正康, 前原 一満, 原田 哲仁, 宮脇 恒太, 河野 健太郎, 島 隆宏, 吉見 智彦, 立花 太郎, 赤司 浩一, 長崎 正朗, 大川 恭行
Organizer
第35回日本分子生物学会年会
Place of Presentation
福岡国際会議場・マリンメッセ福岡（福岡）
Related Report
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