Constitutive approach to the translation system from wheat
| Project/Area Number |
23310141
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
System genome science
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| Research Institution | Ehime University |
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Principal Investigator |
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| Research Collaborator |
HISAMATSU Keigo
TOMIKAWA Chie
KISHIMOTO Tatsuro
KANNO Keisuke
UENO Hidemichi
KONDO Masaki
TOKUMARU Tatsuya
NONAKA Taku
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| Project Period (FY) |
2011-04-01 – 2015-03-31
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| Project Status |
Completed (Fiscal Year 2014)
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| Budget Amount *help |
¥19,630,000 (Direct Cost: ¥15,100,000、Indirect Cost: ¥4,530,000)
Fiscal Year 2014: ¥3,250,000 (Direct Cost: ¥2,500,000、Indirect Cost: ¥750,000)
Fiscal Year 2013: ¥3,380,000 (Direct Cost: ¥2,600,000、Indirect Cost: ¥780,000)
Fiscal Year 2012: ¥3,380,000 (Direct Cost: ¥2,600,000、Indirect Cost: ¥780,000)
Fiscal Year 2011: ¥9,620,000 (Direct Cost: ¥7,400,000、Indirect Cost: ¥2,220,000)
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| Keywords | コムギ / タンパク質合成 / 再構成 / 組換え発現 / タンパク質合成系 / 翻訳 / 翻訳因子 / リボソーム / 合成生物学 / 植物 / 無細胞再構成 |
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| Outline of Final Research Achievements |
Factors involving in translation in wheat were purified one by one, in order to facilitate constructive approaches to the mechanisms in eukaryotic protein synthesis. Samples of acceptable quality were obtained concerning the ribosomes, the 2 eukaryotic elongation factors, 7 of the 14 eukaryotic initiation factors, eukaryotic release factor (eRF) 1, and 4 of the 20 aminoacyl-tRNA synthetases. As no cDNA clone for eRF3 was found in the library of wheat cDNA clones by the National Bio-Resource Project, which had been in progress in parallel with this project, we obtained a clone from wheat germ.
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Report
(5 results)
Research Products
(12 results)
