| Project/Area Number |
23310152
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Living organism molecular science
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| Research Institution | Kyushu University |
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Principal Investigator |
ISHINO YOSHIZUMI 九州大学, (連合)農学研究科(研究院), 教授 (30346837)
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| Co-Investigator(Kenkyū-buntansha) |
ATOMI Haruyuki 京都大学, 大学院・工学研究科, 教授 (90243047)
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| Project Period (FY) |
2011-04-01 – 2014-03-31
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| Project Status |
Completed (Fiscal Year 2013)
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| Budget Amount *help |
¥19,890,000 (Direct Cost: ¥15,300,000、Indirect Cost: ¥4,590,000)
Fiscal Year 2013: ¥5,980,000 (Direct Cost: ¥4,600,000、Indirect Cost: ¥1,380,000)
Fiscal Year 2012: ¥5,590,000 (Direct Cost: ¥4,300,000、Indirect Cost: ¥1,290,000)
Fiscal Year 2011: ¥8,320,000 (Direct Cost: ¥6,400,000、Indirect Cost: ¥1,920,000)
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| Keywords | アーキア / DNA複製 / 極限環境生命 / DNA修復 / 超す熱性アーキア / 生体高分子 / レプリソーム / 超好熱性アーキア |
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| Research Abstract |
Proteins and enzymes involved in DNA replication and repair in the hyperthermophilic archaea, Thermococcus kodakarensis and Pyrococcus furious were characterized by both biochemical and genetical techniques. There are three Mcm and two PCNA in T. kodakarensis, and to understand the functional sharing, we characterized the purified Mcm and PCNA proteins. In addition, we isolated amutant T. kodakarensis strains, in which one of these genes wa knocked out, and characterized their phenotypes. We found that Mcm3 and PCNA1 are essential for the viability of T. kodakarensis. Exact functions of Mcm1, Mcm2, and PCNA2 remain to be elucidated. We searched for enzymes involved in the damaged base repair system in the hyperthermophilic archaea. We identified a novel endonuclease, which cleaves at 5'-side of the damaged nucleotide, hypoxanthine, uracil, xanthine, and AP site in the DNA strand. We designated it Endonuclease Q and applied for a patent as a novel substance from Kyushu University.
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