How is the genome integrity of the hyperthermophiles protected and propagated?
Project/Area Number |
23310152
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Research Category |
Grant-in-Aid for Scientific Research (B)
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Allocation Type | Single-year Grants |
Section | 一般 |
Research Field |
Living organism molecular science
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Research Institution | Kyushu University |
Principal Investigator |
ISHINO YOSHIZUMI 九州大学, (連合)農学研究科(研究院), 教授 (30346837)
|
Co-Investigator(Kenkyū-buntansha) |
ATOMI Haruyuki 京都大学, 大学院・工学研究科, 教授 (90243047)
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Project Period (FY) |
2011-04-01 – 2014-03-31
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Project Status |
Completed (Fiscal Year 2013)
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Budget Amount *help |
¥19,890,000 (Direct Cost: ¥15,300,000、Indirect Cost: ¥4,590,000)
Fiscal Year 2013: ¥5,980,000 (Direct Cost: ¥4,600,000、Indirect Cost: ¥1,380,000)
Fiscal Year 2012: ¥5,590,000 (Direct Cost: ¥4,300,000、Indirect Cost: ¥1,290,000)
Fiscal Year 2011: ¥8,320,000 (Direct Cost: ¥6,400,000、Indirect Cost: ¥1,920,000)
|
Keywords | アーキア / DNA複製 / 極限環境生命 / DNA修復 / 超す熱性アーキア / 生体高分子 / レプリソーム / 超好熱性アーキア |
Research Abstract |
Proteins and enzymes involved in DNA replication and repair in the hyperthermophilic archaea, Thermococcus kodakarensis and Pyrococcus furious were characterized by both biochemical and genetical techniques. There are three Mcm and two PCNA in T. kodakarensis, and to understand the functional sharing, we characterized the purified Mcm and PCNA proteins. In addition, we isolated amutant T. kodakarensis strains, in which one of these genes wa knocked out, and characterized their phenotypes. We found that Mcm3 and PCNA1 are essential for the viability of T. kodakarensis. Exact functions of Mcm1, Mcm2, and PCNA2 remain to be elucidated. We searched for enzymes involved in the damaged base repair system in the hyperthermophilic archaea. We identified a novel endonuclease, which cleaves at 5'-side of the damaged nucleotide, hypoxanthine, uracil, xanthine, and AP site in the DNA strand. We designated it Endonuclease Q and applied for a patent as a novel substance from Kyushu University.
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Report
(4 results)
Research Products
(103 results)
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[Journal Article] A novel single-strand specific 3'-5' exonuclease found in the hyperthermophilic archaeon,, Pyrococcus furiosus2013
Author(s)
Tori, K., Ishino, S., Kiyonari, S., Tahara, S. and Ishino, Y
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Journal Title
Related Report
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[Journal Article] A guanine nucleotide exchange factor for Rab5 proteins is essential for intracellular transport of the proglutelin from the Golgi apparatus to the protein storage vacuole in rice endosperm2013
Author(s)
Masako Fukuda, Liuying Wen, Mio Satoh-Cruz, Yasushi Kawagoe, Yoshiaki Nagamura, Thomas W. Okita, Haruhiko Washida, Aya Sugino, Sonoko Ishino, Yoshizumi Ishino, Masahiro Ogawa, Mariko Sunada, Takashi Ueda, and Toshihiro Kumamaru
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Journal Title
Plant Physiol.
Volume: 162
Issue: 2
Pages: 663-674
DOI
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Peer Reviewed
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[Journal Article] Comparative analyses of the two PCNAs from the hyperthermophilic archaeon, Thermococcus kodakarensis2012
Author(s)
Kuba, Y., Ishino, S., Yamagami, T., Tokuhara, M., Kanai, T., Fujikane, R.,Daiyasu., Atomi, H., Ishino, Y.
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Journal Title
Genes Cells
Volume: 17
Pages: 923-937
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[Journal Article] Biochemical and genetical analyses of the three mcm genes from the hyperthermophilic archaeon, Thermococcus kodakarensis2011
Author(s)
Ishino, S., Fujino, S., Tomita, H., Ogino, H., Takao, K., Daiyasu, H., Kanai, T., Atomi, H., and Ishino, Y.
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Journal Title
Genes Cells
Volume: 16
Pages: 1176-1189
Related Report
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[Journal Article] The GINS complex from the thermoacidophilic archaeon, Thermoplasma acidophilum may function as a homotetramer in DNA replication2011
Author(s)
Ogino, H., Ishino, S. Mayanagi, K., Haugland,G, H., Birkeland, N-K., Yamagishi, A., and Ishino Y
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Journal Title
Extremophiles
Volume: 15
Pages: 529-539
Related Report
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[Journal Article] Architectures of archaeal GINS complexes, essential DNA replication initiation factors2011
Author(s)
Oyama, T, Ishino, S, Fujino, S, Ogino, H, Shirai, T, Mayanagi, K, Saito, M, Nagasawa, N, Ishino, and Y, Morikawa, K.
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Journal Title
BMC Biol
Volume: 9
Pages: 28-28
Related Report
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[Journal Article] Reverse-Chaperoning Activity of an AAA+ Protein2011
Author(s)
Liu, C., McKinney, MC., Chen, YH., Earnest, TM., Shi, X., Lin, LJ., Ishino, Y., Dahmen, K., Cann, I.K., Ha, T
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Journal Title
Biophys J
Volume: 100
Pages: 1344-1352
Related Report
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[Journal Article] Architecture of the DNA polymerase B-proliferating cell nuclear antigen (PCNA)-DNA ternary complex2011
Author(s)
Mayanagi, K., Kiyonari, S., Nishida, H., Saito, M., Kohda, D., Ishino, Y., Shirai, T., and Morikawa, K
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Journal Title
Proc. Natl. Acad. Sci. USA
Volume: 108
Pages: 1845-1849
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[Presentation] Activation mechanism of the replicative helicase in the thermophilic archaeon, Thermoplasma acidophilum.2013
Author(s)
Hiromi Ogino, Sonoko Ishino, Kouta Mayanagi, Takuji Oyama, Tsuyoshi Shirai, Kosuke Morikawa, Gyri Teien Haugland, Nils-Kare Birkeland, and Yoshizumi Ishino
Organizer
Thermophiles 2013
Place of Presentation
Regensburg, Germany
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[Presentation] The synergistic stimulation of the MCM activity by Cdc6/Orc1 and GINS homotetramer from the archaeon, Thermoplasma acidophilum2012
Author(s)
Hiromi Ogino, Sonoko Ishino, Kouta Mayanagi, Takuji Oyama, Tsuyoshi Shirai, Kosuke Morikawa, Gyri Teien Haugland, Nils-Kåre Birkeland, and Yoshizumi Ishino
Organizer
第35回日本分子生物学会年会
Place of Presentation
福岡国際会議場,福岡
Related Report
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[Presentation] Divergently evolved replicative helicase from the archaeon2012
Author(s)
Hiromi Ogino, Sonoko Ishino, Kouta Mayanagi, Takuji Oyama, Tsuyoshi Shirai, Kosuke Morikawa, Gyri Teien Haugland, Nils-Kåre Birkeland, and Yoshizumi Ishino
Organizer
Thermoplasma acidophilum The 8th 3R symposium
Place of Presentation
Awaji Yumebutai International Conference Center, Japan
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