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Development of tissue peptidome analysis technology and its application to discovery of new biologically active peptides

Research Project

Project/Area Number 23310155
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Research Field Living organism molecular science
Research InstitutionNational Cardiovascular Center Research Institute

Principal Investigator

MINAMINO Naoto  独立行政法人国立循環器病研究センター, 研究所, 部長 (50124839)

Co-Investigator(Kenkyū-buntansha) SASAKI Kazuki  独立行政法人国立循環器病研究センター, 研究所, 室長 (80260313)
MOCHIZUKI Akikazu  独立行政法人国立循環器病研究センター, 研究所, 研究員 (30589601)
Co-Investigator(Renkei-kenkyūsha) TAKAO Toshifumi  大阪大学, 蛋白質研究所, 教授 (10197048)
OSAKI Tsukasa  独立行政法人国立循環器病研究センター, 研究所, 特任研究員 (60380565)
Project Period (FY) 2011-04-01 – 2014-03-31
Project Status Completed (Fiscal Year 2013)
Budget Amount *help
¥19,760,000 (Direct Cost: ¥15,200,000、Indirect Cost: ¥4,560,000)
Fiscal Year 2013: ¥6,370,000 (Direct Cost: ¥4,900,000、Indirect Cost: ¥1,470,000)
Fiscal Year 2012: ¥6,370,000 (Direct Cost: ¥4,900,000、Indirect Cost: ¥1,470,000)
Fiscal Year 2011: ¥7,020,000 (Direct Cost: ¥5,400,000、Indirect Cost: ¥1,620,000)
Keywordsペプチドーム解析 / 生理活性ペプチド / 分解ペプチド / 内在ペプチド / 質量分析 / プロセシング / 標識法 / 分解ペプチド標識法 / ペプチド / 組織ペプチドーム解析
Research Abstract

Biologically active peptides (BAPs) are well known as key regulators in the signaling between cells, and used for drugs and diagnosis. Thus, a data set of whole peptides (peptidome) in the cell or tissue is a promising resource for future development of drug and diagnosis. Mass spectrometry has enabled us to analyze numerous peptides, but proteases still hamper the peptidome analysis, i.e. they quickly degraded peptides and converted proteins into peptides. We have succeeded in analyzing peptides in the conditioned media of cultured cells in their intact forms, but not in the tissues. We have recently proposed a new approach to specifically label degraded peptides using a small membrane-permeable reagent, and developed the labeling method in this study. Although the labeling ratio is lower than expected, we can exclude the degraded peptides from the list of identified peptides. This method will facilitate cataloging the endogenous peptides and discovery of new BAPs.

Report

(4 results)
  • 2013 Annual Research Report   Final Research Report ( PDF )
  • 2012 Annual Research Report
  • 2011 Annual Research Report
  • Research Products

    (50 results)

All 2014 2013 2012 2011 Other

All Journal Article (15 results) (of which Peer Reviewed: 15 results,  Open Access: 1 results) Presentation (23 results) (of which Invited: 6 results) Book (6 results) Remarks (6 results)

  • [Journal Article] Proteomic analysis of proteins eliminated by low-density lipoprotein apheresis.2014

    • Author(s)
      Yuasa Y, Osaki T, Makino H, Iwamoto N, Kishimoto I, Usami M, Minamino N, Harada-Shiba M.
    • Journal Title

      Ther. Apher. Dial.

      Volume: 18 Issue: 1 Pages: 93-102

    • DOI

      10.1111/1744-9987.12056

    • Related Report
      2013 Annual Research Report
    • Peer Reviewed
  • [Journal Article] The effects of super-flux (high performance) dialyzer on plasma glycosylated pro-B-type natriuretic peptide (proBNP) and glycosylated N-Terminal proBNP in end-stage renal disease patients on dialysis.2014

    • Author(s)
      Nakagawa Y, Nishikimi T, Kuwahara K, Yasuno S, Kinoshita H, Kuwabara Y, Nakao K, Minami T, Yamada C, Ueshima K, Ikeda Y, Okamoto H, Horii K, Nagata K, Kangawa K, Minamino N, Nakao K.
    • Journal Title

      PLoS One

      Volume: 25 Issue: 3 Pages: e92314-e92314

    • DOI

      10.1371/journal.pone.0092314

    • NAID

      120007100132

    • Related Report
      2013 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Large-scale identification of endogenous secretory peptides using electron transfer dissociation mass spectrometry2013

    • Author(s)
      Sasaki K, Osaki T, Minamino N
    • Journal Title

      Mol Cell Proteomics

      Volume: 12 Pages: 700-709

    • Related Report
      2013 Final Research Report 2012 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Continuous stress promotes expression of VGF in melanotroph via suppression of dopamine2013

    • Author(s)
      Tokizane K, Sasaki K, Minamino N, Kiyama H, 他3名
    • Journal Title

      Mol Cell Endocrinol

      Volume: 372 Pages: 49-56

    • Related Report
      2013 Final Research Report
    • Peer Reviewed
  • [Journal Article] Global metabolomic analysis of heart tissue in a hamster model for dilated cardiomyopathy.2013

    • Author(s)
      Maekawa K, Hirayama A, Iwata Y, Tajima Y, Nishimaki-Mogami T, Sugawara S, Ueno N, Abe H, Ishikawa M, Murayama M, Matsuzawa Y, Nakanishi H, Ikeda K, Arita M, Taguchi R, Minamino N, Wakabayashi S, Soga T, Saito Y.
    • Journal Title

      J Mol Cell Cardiol

      Volume: 59 Pages: 76-85

    • DOI

      10.1016/j.yjmcc.2013.02.008

    • Related Report
      2013 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Continuous stress promotes expression of VGF in melanotroph via suppression of dopamine2013

    • Author(s)
      Kyohei Tokizane
    • Journal Title

      Molecular and Cellular Endocrinology

      Volume: 372 Issue: 1-2 Pages: 4956-4956

    • DOI

      10.1016/j.mce.2013.03.012

    • Related Report
      2013 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Hexarelin treatment in male ghrelin knockout mice after myocardial infarction2013

    • Author(s)
      Mao Y, Tokudome T, Kishimoto I, Otani K, Hosoda H, Nagai C, Minamino N, Miyazato M, Kangawa K
    • Journal Title

      Endocrinology

      Volume: 154 Issue: 10 Pages: 3847-3845

    • DOI

      10.1210/en.2013-1291

    • Related Report
      2013 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Complexity of molecular forms of B-type natriuretic peptide in heart failure.2013

    • Author(s)
      Nishikimi T, Kuwahara K, Nakagawa Y, Kangawa K, Minamino N, Nakao K.
    • Journal Title

      Heart

      Volume: 99 Issue: 10 Pages: 677-679

    • DOI

      10.1136/heartjnl-2012-302929

    • Related Report
      2013 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Molecular characterization and biological function of neuroendocrine regulatory peptide-3 in the rat2012

    • Author(s)
      Fujihara H, Sasaki K, Ueta Y, Minamino N, 他4名
    • Journal Title

      Endocrinology

      Volume: 153 Pages: 1377-1386

    • Related Report
      2013 Final Research Report 2011 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Neuroendocrine regulatory peptide-2 stimulates glucose-induced insulin secretion in vivo and in vitro2012

    • Author(s)
      Moin AS, Yamaguchi H, Sasaki K, Minamino N, 他8名
    • Journal Title

      Biochem Biophys Res Commun

      Volume: 428 Pages: 512-517

    • Related Report
      2013 Final Research Report
    • Peer Reviewed
  • [Journal Article] Neuroendocrine regulatory peptide-2 stimulates glucose-induced insulin secretion in vivo and in vitro.2012

    • Author(s)
      Moin AS, Yamaguchi H, Rhee M, Kim JW, Toshinai K, Waise TM, Naznin F, Matsuo T, Sasaki K, Minamino N, Yoon KH, Nakazato M
    • Journal Title

      Biochem Biophys Res Commun

      Volume: 428 Issue: 4 Pages: 512-517

    • DOI

      10.1016/j.bbrc.2012.10.073

    • Related Report
      2012 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Neuromedin B stimulates proliferation of mouse chondrogenic cell line ATDC52012

    • Author(s)
      Hiroki Saito
    • Journal Title

      Peptides

      Volume: 36 Issue: 2 Pages: 299-302

    • DOI

      10.1016/j.peptides.2012.05.010

    • Related Report
      2012 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Peptidomics-based discovery of an antimicrobial peptide derived from insulinlike growth factor-binding protein 52011

    • Author(s)
      Osaki T, Sasaki K, Minamino N
    • Journal Title

      J Proteome Res

      Volume: 10 Pages: 1870-1880

    • Related Report
      2013 Final Research Report
    • Peer Reviewed
  • [Journal Article] Peptidomics-based discovery of an antimicrobial peptide derived from insulin-like growth factor-binding protein 52011

    • Author(s)
      T.Osaki, K.Sasaki, N.Minamino
    • Journal Title

      Journal of Proteome Research

      Volume: 10 Issue: 4 Pages: 1870-1880

    • DOI

      10.1021/pr101114a

    • Related Report
      2011 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Sustained-release adrenomedullin ointment accelerates wound healing of pressure ulcers2011

    • Author(s)
      K.Harada, N.Minamino, T.Ikeda, et al
    • Journal Title

      Regulatry Peptide

      Volume: 168 Issue: 1-3 Pages: 21-26

    • DOI

      10.1016/j.regpep.2011.02.014

    • Related Report
      2011 Annual Research Report
    • Peer Reviewed
  • [Presentation] ペプチドーム解析に基づく新規生理活性ペプチドの探索2013

    • Author(s)
      南野直人
    • Organizer
      第86回日本内分泌学会学術総会
    • Place of Presentation
      仙台国際センター
    • Related Report
      2013 Final Research Report
  • [Presentation] Peptidomics for naturally occurring secretory peptides using electron transfer dissociation (ETD) mass spectrometry2013

    • Author(s)
      Sasaki K, Osaki T, Minamino N
    • Organizer
      12th HUPO World Congress
    • Place of Presentation
      パシフィコ横浜
    • Related Report
      2013 Final Research Report
  • [Presentation] Peptidomic survey of peptides found in culture supernatant of cardiac fibroblast -an expanded application to common cell having the constitutive secretory pathway2013

    • Author(s)
      Tsuchiya T, Sasaki K, Osaki T, Minamino N
    • Organizer
      4th Asia Pacific International Peptide Symposium
    • Place of Presentation
      ホテル阪急エキスポパーク,大阪
    • Related Report
      2013 Final Research Report
  • [Presentation] Proteomic analysis of left ventricular tissues in dilated cardiomyopathy mouse models2013

    • Author(s)
      Nishigori M, Yagi H, Mochizuki A, Sasaki K, Iwata Y, Wakabayashi S, Minamino N.
    • Organizer
      12th HUPO World Congress
    • Place of Presentation
      横浜、パシフィコ横浜
    • Related Report
      2013 Annual Research Report
  • [Presentation] Peptidomics for naturally occurring secretory peptides using electron transfer dissociation (ETD) mass spectrometry2013

    • Author(s)
      Sasaki K, Osaki T, Minamino N.
    • Organizer
      12th HUPO World Congress
    • Place of Presentation
      横浜、パシフィコ横浜
    • Related Report
      2013 Annual Research Report
  • [Presentation] オミックス解析技術を用いた新規血管作動性ペプチド、タンパク質の探索2013

    • Author(s)
      南野直人、尾崎司、八木寛陽、錦織充広、高山健太郎、佐々木一樹
    • Organizer
      第36回日本高血圧学術総会シンポジウム「血管作動性物質研究の最前線」
    • Place of Presentation
      大阪、大阪国際会議場
    • Related Report
      2013 Annual Research Report
    • Invited
  • [Presentation] 生理活性ペプチド探索法とペプチドーム解析2013

    • Author(s)
      南野直人
    • Organizer
      第17回日本心血管内分泌代謝学会学術総会シンポジウム「治療標的となる心血管ホルモン・タンパク質の発見」
    • Place of Presentation
      大阪、千里ライフサイエンスセンター
    • Related Report
      2013 Annual Research Report
    • Invited
  • [Presentation] BNPの測定意義とナトリウム利尿ペプチドの測定意義2013

    • Author(s)
      南野直人
    • Organizer
      第17回日本心血管内分泌代謝学会学術総会イブニングセミナー「BNPの臨床応用の現状と最新知見」
    • Place of Presentation
      大阪、千里ライフサイエンスセンター
    • Related Report
      2013 Annual Research Report
    • Invited
  • [Presentation] ペプチドーム解析とペプチドの潜在能力の探索2012

    • Author(s)
      南野直人
    • Organizer
      第15回ペプチドフォーラム、地球生命にとってのペプチドの
    • Place of Presentation
      滋賀、長浜バイオ大学
    • Year and Date
      2012-03-16
    • Related Report
      2011 Annual Research Report
  • [Presentation] ペプチドーム探索2012

    • Author(s)
      南野直人
    • Organizer
      第9回GPCR研究会
    • Place of Presentation
      日本未来館, 東京
    • Related Report
      2013 Final Research Report
  • [Presentation] VGFderived peptides identified by mass spectrometry and their functions2012

    • Author(s)
      Sasaki K, Ueta Y, Minamino N
    • Organizer
      3rd Meeting of Japan Branch of the International Neuropeptide Society
    • Place of Presentation
      北九州コンベンションセンター
    • Related Report
      2013 Final Research Report
  • [Presentation] ラット脳内におけるNERP 類の分布,局在とバソプレシン分泌に及ぼす効果2012

    • Author(s)
      望月明和, 佐々木一樹, 中里雅光, 上田陽一, 塩田清二, 南野直人
    • Organizer
      第16回日本心血管内分泌代謝学会学術総会
    • Place of Presentation
      東京大学
    • Related Report
      2013 Final Research Report
  • [Presentation] ペプチドーム探索2012

    • Author(s)
      南野直人
    • Organizer
      第9回GPCR研究会
    • Place of Presentation
      東京、日本未来科学館
    • Related Report
      2012 Annual Research Report
    • Invited
  • [Presentation] VGF-derived peptides identified by mass spectrometry and their functions2012

    • Author(s)
      佐々木一樹
    • Organizer
      The 3rd meeting of the Japan Branch of the International Neuropeptide Society
    • Place of Presentation
      小倉、北九州国際カンファレンスセンター
    • Related Report
      2012 Annual Research Report
    • Invited
  • [Presentation] 神経内分泌制御ペプチド(NERP):生体内におけるプロセシング、分布とバソプレシン分泌制御機能2012

    • Author(s)
      望月明和
    • Organizer
      第49回ペプチド化学討論会
    • Place of Presentation
      鹿児島、かごしま県民交流センター
    • Related Report
      2012 Annual Research Report
  • [Presentation] Discovery and function of secreted peptides2011

    • Author(s)
      Naoto Minamino
    • Organizer
      Neuroscience 2011,米国神経科学会シンポジウム
    • Place of Presentation
      米国Washington, DC Convention Center(招待講演)
    • Year and Date
      2011-11-15
    • Related Report
      2011 Annual Research Report
  • [Presentation] Secretopeptidome and discovery of new biologically active peptides2011

    • Author(s)
      N.Minamino, T.Osaki, K.Sasaki
    • Organizer
      9th Australian Peptide Conference
    • Place of Presentation
      オーストラリア,Hamilton Island(招待講演)
    • Year and Date
      2011-10-17
    • Related Report
      2011 Annual Research Report
  • [Presentation] 内在ペプチドと創薬への応用2011

    • Author(s)
      南野直人
    • Organizer
      第318回情報計算化学生物学会講演会「ペプチド創薬の現状と未来」
    • Place of Presentation
      東京大学山上会館
    • Year and Date
      2011-07-05
    • Related Report
      2013 Final Research Report
  • [Presentation] 内在ペプチドと創薬への応用2011

    • Author(s)
      南野直人
    • Organizer
      第318回情報計算化学生物学会(CBI学会)講演会「ペプチド創薬の現状と未来」
    • Place of Presentation
      東京、東京大学山上会館(招待講演)
    • Year and Date
      2011-07-05
    • Related Report
      2011 Annual Research Report
  • [Presentation] セクレトペプチドーム解析に基づく新規生理活性ペプチド探索2011

    • Author(s)
      佐々木一樹, 高橋憲行, 佐藤光男, 山崎基生, 南野直人
    • Organizer
      第84回日本内分泌学会学術総会
    • Place of Presentation
      神戸国際会議場
    • Related Report
      2013 Final Research Report
  • [Presentation] Peptidomics-based discovery of an antimicrobial peptide derived from insulin-like growth factor binding protein 52011

    • Author(s)
      Osaki T, Sasaki K, Minamino N
    • Organizer
      第48回ペプチド討論会
    • Place of Presentation
      札幌コンベンションセンター
    • Related Report
      2013 Final Research Report
  • [Presentation] Secretopeptidome and discovery of new biologically active peptides2011

    • Author(s)
      Minamino N, Osaki T, Sasaki K
    • Organizer
      9th Australian Peptide Conference
    • Place of Presentation
      Hamilton Island, Australia
    • Related Report
      2013 Final Research Report
  • [Presentation] プロテオームなどのビッグデータの解釈と創薬医療への活用

    • Author(s)
      南野直人
    • Organizer
      京都バイオ計測センターシンポジウム「生命のビッグテータの解釈とその社会への展開」
    • Place of Presentation
      京都、京都リサーチパーク
    • Related Report
      2013 Annual Research Report
    • Invited
  • [Book] Peptidomics strategy for discovering endogenous biologically active peptides2013

    • Author(s)
      Sasaki K, Minamino, N
    • Publisher
      Academic Press/Elsevier
    • Related Report
      2013 Final Research Report
  • [Book] Handbook of the Biologically Active Peptides, Second Edition2013

    • Author(s)
      Minamino N, Nishikimi T.
    • Total Pages
      8
    • Publisher
      Academic Press/Elsevier
    • Related Report
      2013 Annual Research Report
  • [Book] Handbook of the Biologically Active Peptides, Second Edition2013

    • Author(s)
      Sasaki K, Minamino N.
    • Total Pages
      8
    • Publisher
      Academic Press/Elsevier
    • Related Report
      2013 Annual Research Report
  • [Book] ペプチドミクスを活用する新規生理活性ペプチドの探索2012

    • Author(s)
      佐々木一樹, 尾崎司, 南野直人
    • Publisher
      シーエムシー出版
    • Related Report
      2013 Final Research Report
  • [Book] ペプチドミクスを活用する新規生理活性ペプチドの探索、ペプチド医薬の最前線2012

    • Author(s)
      佐々木一樹
    • Total Pages
      4
    • Publisher
      シーエムシー出版
    • Related Report
      2012 Annual Research Report
  • [Book] 質量分析法を活用する生理活性ペプチドの探索- Neuroendocrine Regulatory Peptides の発見2011

    • Author(s)
      佐々木一樹, 山口秀樹, 中里雅光, 南野直人
    • Publisher
      日本臨床化学会
    • Related Report
      2013 Final Research Report
  • [Remarks]

    • URL

      http://www.ncvc.go.jp/res/divisions/pharmacology/001/index.html#-a

    • Related Report
      2013 Final Research Report
  • [Remarks] ペプチドーム解析に基づいた生理活性ペプチドの探索

    • URL

      http://www.ncvc.go.jp/res/divisions/pharmacology/001/index.html#-a

    • Related Report
      2013 Annual Research Report
  • [Remarks] ペプチドーム解析に基づいた生理活性ペプチドの探索の詳細

    • URL

      http://www.ncvc.go.jp/res/divisions/pharmacology/001/a-detail.html

    • Related Report
      2013 Annual Research Report
  • [Remarks] ペプチドーム解析に基づいた生理活性ペプチドの探索

    • URL

      http://www.ncvc.go.jp/res/divisions/pharmacology/001/index.html#-a

    • Related Report
      2012 Annual Research Report
  • [Remarks] ペプチドーム解析に基づいた生理活性ペプチドの探索の詳細

    • URL

      http://www.ncvc.go.jp/res/divisions/pharmacology/001/a-detail.html

    • Related Report
      2012 Annual Research Report
  • [Remarks]

    • URL

      http://www.ncvc.go.jp/res/divisions/pharmacology/001/index.html

    • Related Report
      2011 Annual Research Report

URL: 

Published: 2011-04-06   Modified: 2019-07-29  

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