Development of high throughput screening method of G-quadruplexes using small molecules
| Project/Area Number |
23310158
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Chemical biology
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| Research Institution | Tokyo University of Agriculture and Technology |
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Principal Investigator |
NAGASAWA Kazuo 東京農工大学, 工学(系)研究科(研究院), 教授 (10247223)
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| Project Period (FY) |
2011-04-01 – 2014-03-31
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| Project Status |
Completed (Fiscal Year 2013)
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| Budget Amount *help |
¥19,630,000 (Direct Cost: ¥15,100,000、Indirect Cost: ¥4,530,000)
Fiscal Year 2013: ¥6,240,000 (Direct Cost: ¥4,800,000、Indirect Cost: ¥1,440,000)
Fiscal Year 2012: ¥6,240,000 (Direct Cost: ¥4,800,000、Indirect Cost: ¥1,440,000)
Fiscal Year 2011: ¥7,150,000 (Direct Cost: ¥5,500,000、Indirect Cost: ¥1,650,000)
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| Keywords | グアニン四重鎖 / テロメア / DNAマイクロアレイ / テロメスタチン / 蛍光プローブ / 結合タンパク質 / グアニン四重鎖構造 / テロメア配列 / G-quadruplex / ガン化学療法 |
|---|
| Research Abstract |
G-Quadruplexes (G4s) are three-dimensional structures that are formed in guanine-rich polynucleotides. Relatively few G4-forming oligonucleotides (GFOs) have been identified so far, but they are important because they play significant roles in regulation of gene expression and replication. Computational analysis suggests huge numbers of GFOs are present in the genome. However, there has been no simple and direct screening methodology for large-scale screening of GFOs.
In this project, we have developed a high-throughput screening method for GFOs by using the fluorescent G4 ligand L1Cy5-7OTD with a DNA microarray. With this method, we found 1,998 novel GFOs in a CpG island (CGI) microarray bearing 88,737 targets. Gene Ontology (GO) analysis suggests that these GFOs were mainly associate with metabolic processes, transcriptional regulation, biosynthetic processes and developmental processes. The present method would be easily applicable to other DNA microarrays.
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Report
(4 results)
Research Products
(118 results)
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[Journal Article] A nonclassical vitamin D receptor pathway suppresses renal fibrosis2013
Author(s)
Ito I, Waku T, Aoki M, Abe R, Nagai Y, Watanabe T, Nakajima Y, Ohkido I, Yokoyama K, Miyachi H, Shimizu T, Murayama A, Kishimoto H, Nagasawa K, Yanagisawa J.
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Journal Title
Journal of Clinical Investigation
Volume: 123
Issue: 11
Pages: 4579-4594
DOI
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Peer Reviewed
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[Presentation] Synthetic studies on Lymphostin2013
Author(s)
Tatsuya Yamada, Yohei Takayama, Shinya Tatekabe, Kazuo Nagasawa
Organizer
13th International Conference on the Chemistry of Antibiotics and other bioactive compounds (ICCA-13)
Place of Presentation
富士ビューホテル（山梨県）
Related Report
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[Presentation] Total synthesis of (+)-Cylindradine A2013
Author(s)
Makoto Iwata, Kyohei Kanou, Takuya Imaoka, Kazuo Nagasawa
Organizer
13th International Conference on the Chemistry of Antibiotics and other bioactive compounds (ICCA-13)
Place of Presentation
富士ビューホテル（山梨県）
Related Report
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[Presentation] Synthetic studies on Lymphostin
Author(s)
Tatsuya Yamada, Yohei Takayama, Shinya Tatekabe, Kazuo Nagasawa
Organizer
The 11th International Symposium on Advanced Technology (ISAT-Special)
Place of Presentation
工学院大学八王子キャンパス
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