| Project/Area Number |
23310171
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Resource conservation science
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| Research Institution | National Institute of Advanced Industrial Science and Technology |
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Principal Investigator |
TSUDA SAKAE 独立行政法人産業技術総合研究所, 生物プロセス研究部門, 上級主任研究員 (70211381)
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| Co-Investigator(Kenkyū-buntansha) |
KONDO Hidemasa 産業技術総合研究所, 生物プロセス研究部門, 主任研究員 (80357045)
NISHIMIYA Yoshiyuki 産業技術総合研究所, ・生物プロセス研究部門, 主任研究員 (00357716)
SAKASHITA Mami 産業技術総合研究所, 生物プロセス研究部門, 主任研究員 (20357099)
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| Project Period (FY) |
2011-04-01 – 2014-03-31
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| Project Status |
Completed (Fiscal Year 2013)
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| Budget Amount *help |
¥17,550,000 (Direct Cost: ¥13,500,000、Indirect Cost: ¥4,050,000)
Fiscal Year 2013: ¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2012: ¥5,330,000 (Direct Cost: ¥4,100,000、Indirect Cost: ¥1,230,000)
Fiscal Year 2011: ¥8,320,000 (Direct Cost: ¥6,400,000、Indirect Cost: ¥1,920,000)
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| Keywords | 細胞保護物質 / ペプチド / 不凍蛋白質 / 抗凍結作用 / ３次元分子構造 / 大量生産 / Ｘ線回折法 / ＮＭＲ法 / 細胞保護 / 分子構造 / 氷結晶結合 / 脂質二重膜 / 不凍タンパク質 / 脂質二十膜 |
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| Research Abstract |
Antifreeze proteins (AFPs) found from many organisms can inhibit ice crystal growth for cold-survival. We preliminarily found that some AFPs also show cell-preservation ability and named them CPP (Cell-Preservation Peptide). During the 3-year research period, we have been tested structure-function relationship of various AFPs to obtain the most promising CPP. In 2013, we finally could develop a method to alive rat insulinoma cells for more than 5 days at +4 degree C by using a flat-fish-derived CPP (PLoS ONE, 2013). In addition, we published the 1st crystal structure of fungal AFP (PNAS, 2012) and structure-function of CPP3 based on the multi-dimensional NMR experiments (JBNMR, 2013). Biochechemical characterizations and many applicational results about AFP and CPP were also published: 23 publications (incl.19 peer-reviewed articles), 40 presentations (incl.14 invited speeches), and 1 patent claim.
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