| Budget Amount *help |
¥20,280,000 (Direct Cost: ¥15,600,000、Indirect Cost: ¥4,680,000)
Fiscal Year 2013: ¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000)
Fiscal Year 2012: ¥7,540,000 (Direct Cost: ¥5,800,000、Indirect Cost: ¥1,740,000)
Fiscal Year 2011: ¥8,710,000 (Direct Cost: ¥6,700,000、Indirect Cost: ¥2,010,000)
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| Research Abstract |
In order to understand the mechanism of general anesthesia with Xe, we carried out the mesoscopic and microscopic measurements on cultured neuronal network from rat cortex. We measured the activity depression of cultured neuronal network under Xe gas pressure by multi-electrode arrays (mesoscopic measurement) and by a Raman spectroscopy (microscopic measurement). When we applied Xe gas between 0.1 and 0.5 MPa on cultured neuronal network, the characteristic firing activity (synchronized burst firing) was completely inhibited under the conditions of Xe pressure higher than 0.3 MPa. Raman spectra of neurons showed the peak shift of C-H stretching mode of the cell membranes under Xe pressure, which indicated that Xe molecules dissolved in membranes. This indication coincides with that suggested by MD simulation.
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