| Budget Amount *help |
¥20,280,000 (Direct Cost: ¥15,600,000、Indirect Cost: ¥4,680,000)
Fiscal Year 2013: ¥5,590,000 (Direct Cost: ¥4,300,000、Indirect Cost: ¥1,290,000)
Fiscal Year 2012: ¥5,720,000 (Direct Cost: ¥4,400,000、Indirect Cost: ¥1,320,000)
Fiscal Year 2011: ¥8,970,000 (Direct Cost: ¥6,900,000、Indirect Cost: ¥2,070,000)
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| Research Abstract |
Excess energy generated in an active site in proteins transferred to and dissipates in the protein moiety. Ultraviolet resonance Raman (UVRR) spectroscopy can selectively monitor Raman bands of aromatic amino acid residues, such as tryptophan, tyrosine and phenylalanine. Anti-Stokes Raman intensity reflects the population in vibrationally excited states. Therefore, anti-Stokes UVRR intensity can be a direct probe of vibrational energy of residues in a protein. In this study, we observed the vibrational energy migration in cytochrome b562 and myoglobin by using anti-Stokes UVRR spectroscopy and elucidated distance dependence of the vibrational energy migration.
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