Research on Full-protocol Disease Marker Screening System using Integrated Lab-on-a-CD for Immunoassay
Project/Area Number |
23350035
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Research Category |
Grant-in-Aid for Scientific Research (B)
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Allocation Type | Single-year Grants |
Section | 一般 |
Research Field |
Analytical chemistry
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Research Institution | University of Hyogo |
Principal Investigator |
UTSUMI Yuichi 兵庫県立大学, 高度産業科学技術研究所, 教授 (80326298)
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Co-Investigator(Kenkyū-buntansha) |
TAKEO Masahiro 兵庫県立大学, 大学院・工学研究科, 准教授 (40236443)
YUSA Shinichi 兵庫県立大学, 大学院・工学研究科, 准教授 (00301432)
UKITA Yoshiaki 北陸先端科学技術大学院大学, マテリアルサイエンス研究科, 助教 (40578100)
KATAOKA Masatoshi) 産業技術総合研究所, 健康工学研究部門, グループ長 (20224438)
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Project Period (FY) |
2011-04-01 – 2014-03-31
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Project Status |
Completed (Fiscal Year 2013)
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Budget Amount *help |
¥18,720,000 (Direct Cost: ¥14,400,000、Indirect Cost: ¥4,320,000)
Fiscal Year 2013: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Fiscal Year 2012: ¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000)
Fiscal Year 2011: ¥14,300,000 (Direct Cost: ¥11,000,000、Indirect Cost: ¥3,300,000)
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Keywords | チップ分析 / 生体分析 / 免疫反応 / バイオリアクター / ELISA / マイクロ化学システム / Lab-on-a-chip / Lab-on–a-chip |
Research Abstract |
This paper reports a new type of lab-on-a-CD device with three-dimensional microchannel networks and vertical capillary bundle structure. The device consist of the multiple lab-on-a-CD chips with conventional planer microchannels and vertical microchannels which constructed on the disks made of poly-dimethylsiloxane (PDMS) and poly-methylmethacrilrate (PMMA). The PMMA layer with vertical channel were fabricated by deep X-ray lithography using synchrotron radiation. We designed and fabricated the chips for aim of taking enzyme-linked immunosorbent assay(ELISA). Three-dimensional liquid transportation through Lab-on-a-CD structure is suggested by computational fluid dynamics and it is successfully demonstrated chips. Detection of immunogroblin G(IgG) from mouse is successfully demonstrated in the three-dimensional devices and it is notable that the result suggests quite high-sensitive implying detection limit down to 1ng/mL by primitive result.
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Report
(4 results)
Research Products
(58 results)
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[Presentation] Development of Automatic ELISA Analytical System for a Trace Amount of Nonylphenol Using a Novel Antigen-Bound Microfilter2013
Author(s)
M. Takeo, I. Kawaji, A. Nakasuji, T. Tone, Y. Ukita, D. Kato, S. Negoro, S. Yusa, M. Katayama and Y. Utsumi
Organizer
10th International Workshop on High Aspect Ratio Micro and Nano System Technology
Place of Presentation
SEMINARIS CAMPUSHOTEL BERLIN, Berlin, Germany
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[Presentation] Automatic ELISA Analytical System for a Trace Amount of Environmental Chemicals Using A 3-Dimensional Microreactor with a Novel Antigen-Bound Microfilter2012
Author(s)
Masahiro Takeo, Isao Kawaji, Akihiro Nakasuji, Takaaki Tone, Yoshiaki Ukita, Dai-ichiro Kato, Seiji Negoro, Shinichi Yusa, Makoto Katayama, Yuichi Utsumi
Organizer
16th International Conference on Miniaturized Systems for Chemistry and Life Science (μTAS)
Place of Presentation
Okinawa Convention Center, Okinawa
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