Multifunctional two-photon excitable fluorescence probes for monitoring mitochondrial dynamics
| Project/Area Number |
23350068
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Functional materials chemistry
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| Research Institution | Yamaguchi University |
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Principal Investigator |
KAWAMATA JUN 山口大学, 医学(系)研究科(研究院), 教授 (40214689)
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| Co-Investigator(Kenkyū-buntansha) |
SUZUKI Yasutaka 山口大学, 大学院・医学系研究科, 助教 (30634753)
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| Project Period (FY) |
2011-04-01 – 2014-03-31
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| Project Status |
Completed (Fiscal Year 2013)
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| Budget Amount *help |
¥19,240,000 (Direct Cost: ¥14,800,000、Indirect Cost: ¥4,440,000)
Fiscal Year 2013: ¥5,590,000 (Direct Cost: ¥4,300,000、Indirect Cost: ¥1,290,000)
Fiscal Year 2012: ¥5,590,000 (Direct Cost: ¥4,300,000、Indirect Cost: ¥1,290,000)
Fiscal Year 2011: ¥8,060,000 (Direct Cost: ¥6,200,000、Indirect Cost: ¥1,860,000)
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| Keywords | 非線形光学 / 二光子吸収 / 二光子励起蛍光顕微鏡 / 蛍光プローブ / 蛍光イメージング / 蛍光 / 二光子励起顕微鏡 / ミトコンドリア / バイオイメージング |
|---|
| Research Abstract |
Several fluorescence probes for imaging of live mitochondria have been synthesized. These probes exhibited not only salient two-photon excitation fluorescence efficiencies but also high solubility to water. Owing to the high solubility to water of the novel probes, living cells have been stained without using any organic solvent. This advantage of the present probes enabled long time live cell imaging over than one week with a prominent sensitivity.
In addition, we have developed fluorescence probes which switch accumulated organelle by external stimuli. The probes accumulated at mitochondria when the mitochondrial membrane potential was high. As the cell activity decreased the probes moved from mitochondria to nucleus. Reversible change of accumulated organelle has also been confirmed.
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Report
(4 results)
Research Products
(73 results)
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[Journal Article] Incorporation of cationic electron donor of Ni-pridyltetrathiafulvalene with anionic electron accepter of polyoxometalate2012
Author(s)
R. Tsunashima, T. Matsumoto, N. Hoshino, W. Niiho, M. Kimura, K. Kendo, Y. Suyama, Y. Nishioka, J. Kawamata, S. Noro, T. Nakamura, T, Akutagawa, K. Ishiguro
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Journal Title
Dalton Trans
Volume: 41
Pages: 10060-10060
Related Report
Peer Reviewed
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[Patent(Industrial Property Rights)] 二光子吸収化合物2014
Inventor(s)
鈴木康孝, 川俣純, 守友博紀, 富永亮, 小西玄一, 仁子洋輔
Industrial Property Rights Holder
国立大学法人山口大学
Industrial Property Rights Type
特許
Industrial Property Number
2014-047329
Filing Date
2014-03-11
Related Report
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