| Budget Amount *help |
¥19,370,000 (Direct Cost: ¥14,900,000、Indirect Cost: ¥4,470,000)
Fiscal Year 2013: ¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000)
Fiscal Year 2012: ¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000)
Fiscal Year 2011: ¥10,920,000 (Direct Cost: ¥8,400,000、Indirect Cost: ¥2,520,000)
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| Research Abstract |
The purpose of this study is physical and chemical understanding of RNA maturation system. The study were performed for three years. We mainly researched the relatively simple organism of three domains of life (eukaryotes, archaea and eubacteria). We chracterized several tRNA modification enzymes and tRNA splicing endonulceases by X-ray crystal structural study and biochemical methods. Furthermore, we investigated the network between modified nucleosides in tRNA and tRNA modification enzymes using gene disruptant strains. Moreover, we found distinct tRNA modifications in a thermo-acidophilic archaeon, Thermoplasma acidophilum.
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