Structure and function of sensor proteins that regulate biological functions by gas molecules
Project/Area Number |
23350084
|
Research Category |
Grant-in-Aid for Scientific Research (B)
|
Allocation Type | Single-year Grants |
Section | 一般 |
Research Field |
Chemistry related to living body
|
Research Institution | Okazaki Research Facilities, National Institutes of Natural Sciences |
Principal Investigator |
AONO Shigetoshi 大学共同利用機関法人自然科学研究機構(岡崎共通研究施設), 岡崎統合バイオサイエンスセンター, 教授 (60183729)
|
Project Period (FY) |
2011-11-18 – 2014-03-31
|
Project Status |
Completed (Fiscal Year 2013)
|
Budget Amount *help |
¥10,660,000 (Direct Cost: ¥8,200,000、Indirect Cost: ¥2,460,000)
Fiscal Year 2013: ¥5,200,000 (Direct Cost: ¥4,000,000、Indirect Cost: ¥1,200,000)
Fiscal Year 2012: ¥5,460,000 (Direct Cost: ¥4,200,000、Indirect Cost: ¥1,260,000)
|
Keywords | センサータンパク質 / ヘムタンパク質 / 転写調節因子 / 走化性制御系 / シグナルトランスデューサー / 酸素センサー |
Research Abstract |
A chemotaxis signal transducer protein Aer2 from Pseudomonas aeruginosa was found to be a novel heme-based oxygen sensor protein. The crystal structure of Aer2 was determined, which reveals that a hydrogen bonding interaction between heme-bound oxygen and Trp283 in the distal heme pocket plays an important role for oxygen sensing and signal transduction of Aer2. A novel heme sensor protein HrtR was also found in this work. The molecular mechanisms of heme sensing by HrtR and of the heme-responsive functional regulation of HrtR were elucidated by determining its crystal structure.
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Report
(4 results)
Research Products
(66 results)