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Study on functional development, homeostasis and degradation of lubrication mechanism in living tissue using regenerated cartilage model cultured under traction loading

Research Project

Project/Area Number 23360076
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Research Field Design engineering/Machine functional elements/Tribology
Research InstitutionKyushu University

Principal Investigator

SAWAE Yoshinori  九州大学, 工学(系)研究科(研究院), 教授 (10284530)

Co-Investigator(Kenkyū-buntansha) NAKASHIMA Kazuhiro  九州大学, 大学院・工学研究院, 助教 (70315109)
WATANABE Masao  北海道大学 (30274484)
Project Period (FY) 2011-04-01 – 2014-03-31
Project Status Completed (Fiscal Year 2013)
Budget Amount *help
¥18,980,000 (Direct Cost: ¥14,600,000、Indirect Cost: ¥4,380,000)
Fiscal Year 2013: ¥2,730,000 (Direct Cost: ¥2,100,000、Indirect Cost: ¥630,000)
Fiscal Year 2012: ¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000)
Fiscal Year 2011: ¥12,090,000 (Direct Cost: ¥9,300,000、Indirect Cost: ¥2,790,000)
Keywordsトライボロジー / 細胞・組織 / 関節軟骨 / 潤滑 / 移植・再生医療 / 摩擦 / メカノトランスダクション
Research Abstract

The agarose construct containing freshly isolated primary chondrocytes was prepared as a regenerated cartilage tissue model and cultured under the traction loading applied to the model surface. Then, the effects of the traction loading on the functional development and degradation of lubricious cartilage tissues were examined. Results indicated that the traction loading on the tissue surface could stimulate the biosynthesis of extracellular matrix and bring anisotropic nature in the regenerated cartilage tissue. However, the enhancement in the lubricating ability of cultured tissue surface could not be confirmed experimentally. However, degenerative effects of the excessive shear deformation that would be caused by the disruption of lubrication mechanism could be confirmed, since the cell viability in the agarose construct dropped drastically when the shear strain exceeded 5%.

Report

(4 results)
  • 2013 Annual Research Report   Final Research Report ( PDF )
  • 2012 Annual Research Report
  • 2011 Annual Research Report
  • Research Products

    (35 results)

All 2014 2013 2012 2011

All Journal Article (8 results) (of which Peer Reviewed: 8 results) Presentation (27 results) (of which Invited: 4 results)

  • [Journal Article] Influence of phospholipid and protein constituents on tribological properties of artificial hydrogel cartilage material2013

    • Author(s)
      S. Yarimitsu, K. Nakashima, Y. Sawae, T. Murakami
    • Journal Title

      J.Biomech.Sci. Eng.

      Volume: 8 Pages: 257-267

    • NAID

      130003366829

    • Related Report
      2013 Final Research Report
    • Peer Reviewed
  • [Journal Article] Influence of Phospholipid and Protein Constituents on Tribological Properties of Artificial Hydrogel Cartilage Material2013

    • Author(s)
      S. Yarimitsu, K. Nakashima, Y. Sawae and T. Murakami
    • Journal Title

      Journal of Biomechanical Science and Engineering

      Volume: 8 Issue: 3 Pages: 257-267

    • DOI

      10.1299/jbse.8.257

    • NAID

      130003366829

    • ISSN
      1880-9863
    • Related Report
      2013 Annual Research Report
    • Peer Reviewed
  • [Journal Article] バイオトライボロジーへの導入2012

    • Author(s)
      澤江義則
    • Journal Title

      トライボロジスト

      Volume: 57 Pages: 270-272

    • NAID

      10030540454

    • Related Report
      2013 Final Research Report 2012 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Effects of both vitamin C and mechanical stimulation on improving the mechanical characteristics of regenerated cartilage2012

    • Author(s)
      Omata, S.
    • Journal Title

      Biochemical and Biophysical Research Communications

      Volume: 424 Issue: 4 Pages: 724-729

    • DOI

      10.1016/j.bbrc.2012.07.019

    • Related Report
      2012 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Relationship between magnitude of immediate loading and peri-im plant bone reaction in does2012

    • Author(s)
      ESAKI D, et al
    • Journal Title

      Clin Oral Implants Res

      Volume: (未定) Issue: 11 Pages: 1290-1296

    • DOI

      10.1111/j.1600-0501.2011.02305.x

    • Related Report
      2012 Annual Research Report
    • Peer Reviewed
  • [Journal Article] 未使用大腿骨コンポーネントの微小表面突起と超高分子量ポリエチレンとの接触解析2011

    • Author(s)
      趙昌熙, 他2名
    • Journal Title

      日本臨床バイオメカニクス学会誌

      Volume: 32 Pages: 267-276

    • Related Report
      2011 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Effects of Mucosal Thickness on the Stress Distribution and Denture Stability of Mandibular Implant-Supported Overdentures with Unsplinted Attachments In Vitro2011

    • Author(s)
      A.HARUTA, et.al.
    • Journal Title

      Journal of Dental Biomechanics

      Volume: 2011 Pages: 7-7

    • Related Report
      2011 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Effectiveness of Adsorbed Film and Gel Layer in Hydration Lubrication as Adaptive Multimode Lubrication Mechanism for Articular Cartilage2011

    • Author(s)
      T MURAKAMI, et.al.
    • Journal Title

      Proc.IMechE, Part J

      Volume: 225 Pages: 1174-1185

    • Related Report
      2011 Annual Research Report
    • Peer Reviewed
  • [Presentation] 繰返し力学的刺激が再生軟骨組織モデル内の軟骨細胞の生存率へ与える影響2014

    • Author(s)
      小俣誠二,松下康平,福田圭祐,村上輝夫,澤江義則
    • Organizer
      日本機械学会 第26回バイオエンジニアリング講演会
    • Place of Presentation
      仙台
    • Related Report
      2013 Annual Research Report
  • [Presentation] 摺動負荷が再生軟骨組織のECM 産生へ与える影響2014

    • Author(s)
      福田圭祐,小俣誠二,澤江義則
    • Organizer
      日本機械学会 第26回バイオエンジニアリング講演会
    • Place of Presentation
      仙台
    • Related Report
      2013 Annual Research Report
  • [Presentation] 摩擦負荷培養法による再生軟骨組織形成とその力学特性に関する研究2013

    • Author(s)
      福田圭祐,澤江義則,小俣誠二
    • Organizer
      第36回日本バイオレオロジー学会年会
    • Place of Presentation
      福岡
    • Related Report
      2013 Annual Research Report
  • [Presentation] Effect of Phospholipids on Wear and Friction of Ultra-High Molecular Weight Polyethylene2013

    • Author(s)
      Oide, R., Morioka, T., Sawae, Y.
    • Organizer
      World Tribology Congress 2013
    • Place of Presentation
      Torino, Italy
    • Related Report
      2013 Annual Research Report
  • [Presentation] Behavior of Adsorbed Albumin Film on CoCrMo Alloy under In-situ Observation2013

    • Author(s)
      Nakshima, K., Sawae, Y., Murakami, T.
    • Organizer
      World Tribology Congress 2013
    • Place of Presentation
      Torino, Italy
    • Related Report
      2013 Annual Research Report
  • [Presentation] Effect of Synovial Fluid Constituents on Tribological Performance of Artificial Hydrogel Cartilage Material2013

    • Author(s)
      Yarimitsu, S., Nakashima, K., Sawae, Y., Murakami, T.
    • Organizer
      World Tribology Congress 2013
    • Place of Presentation
      Torino, Italy
    • Related Report
      2013 Annual Research Report
  • [Presentation] Traction Loading on Tissue Surface Stimulate Cartilage Tissue Development by Cultured Chondrocytes2013

    • Author(s)
      Sawae, Y., Fukuda, K., Omata, S.
    • Organizer
      World Tribology Congress 2013
    • Place of Presentation
      Torino, Italy
    • Related Report
      2013 Annual Research Report
  • [Presentation] 再生軟骨組織形成とその力学特性に対する摩擦負荷培養の影響2013

    • Author(s)
      福田圭祐,小俣誠二,澤江義則
    • Organizer
      生体医工学シンポジウム 2013
    • Place of Presentation
      福岡
    • Related Report
      2013 Annual Research Report
  • [Presentation] Development Of Cartilaginous Tissue In Chondrocyte-Agarose Construct Cultured Under Traction Loading2013

    • Author(s)
      Fukuda, K., Omata, S., Sawae, Y.
    • Organizer
      The 15th International Conference on Biomedical Engineering
    • Place of Presentation
      Singapore
    • Related Report
      2013 Annual Research Report
  • [Presentation] 軟骨細胞による組織形成に対する摩擦負荷培養の影響2012

    • Author(s)
      福田圭祐, 他3名
    • Organizer
      日本機械学会第24回バイオエンジニアリング講演会
    • Place of Presentation
      大阪大学(大阪)
    • Year and Date
      2012-01-07
    • Related Report
      2011 Annual Research Report
  • [Presentation] 生体模擬環境における超高分子量ポリエチレン摩耗粉の形態評価2012

    • Author(s)
      森岡卓志
    • Organizer
      トライボロジー会議2012春 東京
    • Place of Presentation
      東京都
    • Related Report
      2012 Annual Research Report
  • [Presentation] Experimental study on wear of UHMWPE in synovial environment2012

    • Author(s)
      Yoshinori Sawae
    • Organizer
      BIOTRIBOLOGY XI'AN 2012
    • Place of Presentation
      Xi'an, China
    • Related Report
      2012 Annual Research Report
    • Invited
  • [Presentation] Biotribology in Natural Synovial Joint and Joint Prosthesis2012

    • Author(s)
      Yoshinori Sawae
    • Organizer
      Malaysia-Japan Tribology Meeting
    • Place of Presentation
      Kuala Lumpur, Malaysia
    • Related Report
      2012 Annual Research Report
  • [Presentation] Effect of Phospholipids on Wear of Ultra-High Molecular Weight Polyethylene2012

    • Author(s)
      Yoshinori Sawae
    • Organizer
      39th Leeds-Lyon Symposium on Tribology
    • Place of Presentation
      Leeds, UK
    • Related Report
      2012 Annual Research Report
  • [Presentation] 生体関節と人工関節における境界潤滑2012

    • Author(s)
      澤江義則
    • Organizer
      トライボロジー会議2012秋 北海道室蘭
    • Place of Presentation
      室蘭市
    • Related Report
      2012 Annual Research Report
    • Invited
  • [Presentation] ハイドロゲル製人工軟骨の摩擦・摩耗挙動に及ぼすリン脂質と蛋白質の影響2012

    • Author(s)
      鎗光清道
    • Organizer
      日本機械学会2012年度年次大会
    • Place of Presentation
      金沢市
    • Related Report
      2012 Annual Research Report
  • [Presentation] 摩擦負荷培養法による再生軟骨組織の形成とその力学特性2012

    • Author(s)
      福田圭介
    • Organizer
      日本機械学会九州支部福岡講演会
    • Place of Presentation
      福岡市
    • Related Report
      2012 Annual Research Report
  • [Presentation] 超高分子量ポリエチレンの摩擦・摩耗に及ぼす脂質の影響2012

    • Author(s)
      森岡卓志
    • Organizer
      日本機械学会九州支部福岡講演会
    • Place of Presentation
      福岡市
    • Related Report
      2012 Annual Research Report
  • [Presentation] Interaction Between Physiological Environment and Surface of Joint Prosthesis - Effect on Friction and Wear2012

    • Author(s)
      Yoshinori Sawae
    • Organizer
      Bio4Apps 2012
    • Place of Presentation
      Singapore
    • Related Report
      2012 Annual Research Report
    • Invited
  • [Presentation] Microscopic Structure Formation in Regenerated Cartilage Tissue Cultured Under Traction Loading2012

    • Author(s)
      Keisuke Fukuda
    • Organizer
      Bio4Apps 2012
    • Place of Presentation
      Singapore
    • Related Report
      2012 Annual Research Report
    • Invited
  • [Presentation] Structure and Mechanical Properties of Regenerated Cartilage Tissue Cultured Under Traction Loading2012

    • Author(s)
      Yoshinori Sawae
    • Organizer
      MJIIT-JUC Joint Symposium 2012
    • Place of Presentation
      Kuala Lumpur, Malaysia
    • Related Report
      2012 Annual Research Report
  • [Presentation] Effect of Mechanical Loading on Chondrocyte Biosynthesis of Extracellular Matrix in Agarose Construct2011

    • Author(s)
      Y.Sawae
    • Organizer
      8th International Conference on Flow Dynamics
    • Place of Presentation
      ホテルメトロポリタン仙台(宮城)(招待講演)
    • Year and Date
      2011-11-11
    • Related Report
      2011 Annual Research Report
  • [Presentation] Development and Evaluation of Friction Property of Hybrid Artificial Cartilage with Extra-Cellular Matrix2011

    • Author(s)
      K.Nakashima, et.al.
    • Organizer
      6th International Biotribology Forum
    • Place of Presentation
      西鉄イン福岡(福岡)
    • Year and Date
      2011-11-05
    • Related Report
      2011 Annual Research Report
  • [Presentation] Influence of Phospholipid and Protein Constituents on Friction and Wear Behavior of Artificial Hydrogel Cartilage Material2011

    • Author(s)
      S.Yarimitsu, et.al.
    • Organizer
      International Tribology Conference Hiroshima 2011
    • Place of Presentation
      広島国際会議場(広島)
    • Year and Date
      2011-11-03
    • Related Report
      2011 Annual Research Report
  • [Presentation] 培養軟骨細胞による再生軟骨組織形成におよぼす摩擦負荷の影響2011

    • Author(s)
      福田圭祐, 他3名
    • Organizer
      日本機械学会九州支部宮崎講演会
    • Place of Presentation
      宮崎大学(宮崎)
    • Year and Date
      2011-09-30
    • Related Report
      2011 Annual Research Report
  • [Presentation] Effects of traction loading on regeneration of cartilage tissue in chondrocyte-agarose construct2011

    • Author(s)
      Y.Sawae, et.al.
    • Organizer
      The International Conference on BioTribology
    • Place of Presentation
      London, UK
    • Year and Date
      2011-09-20
    • Related Report
      2011 Annual Research Report
  • [Presentation] 関節軟骨の構造と力学的機能2011

    • Author(s)
      澤江義則
    • Organizer
      同志社大学生体医療材料研究センター第2回シンポジウム
    • Place of Presentation
      同志社大学(京都)(招待講演)
    • Year and Date
      2011-08-09
    • Related Report
      2011 Annual Research Report

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Published: 2011-04-06   Modified: 2019-07-29  

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