Study on functional development, homeostasis and degradation of lubrication mechanism in living tissue using regenerated cartilage model cultured under traction loading
| Project/Area Number |
23360076
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Design engineering/Machine functional elements/Tribology
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| Research Institution | Kyushu University |
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Principal Investigator |
SAWAE Yoshinori 九州大学, 工学(系)研究科(研究院), 教授 (10284530)
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| Co-Investigator(Kenkyū-buntansha) |
NAKASHIMA Kazuhiro 九州大学, 大学院・工学研究院, 助教 (70315109)
WATANABE Masao 北海道大学 (30274484)
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| Project Period (FY) |
2011-04-01 – 2014-03-31
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| Project Status |
Completed (Fiscal Year 2013)
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| Budget Amount *help |
¥18,980,000 (Direct Cost: ¥14,600,000、Indirect Cost: ¥4,380,000)
Fiscal Year 2013: ¥2,730,000 (Direct Cost: ¥2,100,000、Indirect Cost: ¥630,000)
Fiscal Year 2012: ¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000)
Fiscal Year 2011: ¥12,090,000 (Direct Cost: ¥9,300,000、Indirect Cost: ¥2,790,000)
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| Keywords | トライボロジー / 細胞・組織 / 関節軟骨 / 潤滑 / 移植・再生医療 / 摩擦 / メカノトランスダクション |
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| Research Abstract |
The agarose construct containing freshly isolated primary chondrocytes was prepared as a regenerated cartilage tissue model and cultured under the traction loading applied to the model surface. Then, the effects of the traction loading on the functional development and degradation of lubricious cartilage tissues were examined. Results indicated that the traction loading on the tissue surface could stimulate the biosynthesis of extracellular matrix and bring anisotropic nature in the regenerated cartilage tissue. However, the enhancement in the lubricating ability of cultured tissue surface could not be confirmed experimentally. However, degenerative effects of the excessive shear deformation that would be caused by the disruption of lubrication mechanism could be confirmed, since the cell viability in the agarose construct dropped drastically when the shear strain exceeded 5%.
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Report
(4 results)
Research Products
(35 results)
