Construction of chromosome fiber analysis platform using MEMS technology
| Project/Area Number |
23360114
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Intelligent mechanics/Mechanical systems
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| Research Institution | Kagawa University |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
TERAO Kyohei 香川大学, 工学部, 准教授 (80467448)
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| Project Period (FY) |
2011-04-01 – 2014-03-31
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| Project Status |
Completed (Fiscal Year 2013)
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| Budget Amount *help |
¥16,510,000 (Direct Cost: ¥12,700,000、Indirect Cost: ¥3,810,000)
Fiscal Year 2013: ¥2,860,000 (Direct Cost: ¥2,200,000、Indirect Cost: ¥660,000)
Fiscal Year 2012: ¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000)
Fiscal Year 2011: ¥9,100,000 (Direct Cost: ¥7,000,000、Indirect Cost: ¥2,100,000)
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| Keywords | 情報機器・知能機械システム / BioMEMS / microTAS / 三次元リソグラフィ / 染色体 / 細胞固定 / FISH / ﾏｲｸﾛ・ﾅﾉﾃﾞﾊﾞｲｽ / ﾏｲｸﾛﾏｼﾝ / ﾌｫﾄﾘｿｸﾞﾗﾌｨ / 染色体解析 / 細胞操作 / マイクロ・ナノデバイス / マイクロマシン / フォトリソグラフィ |
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| Research Abstract |
In this study, we propose a simple observation method of the shape and molecular orientation of the chromosomes extracted from cells. The proposed technique is composed of total preparation technique such as cell immobilization, chromosomes extraction, stretching, suspension and analysis using a disposable microchip controlled by centrifugal force only. It is experimentally confirmed that the chip having two kinds of microstructures arranged concentrically on a chip immobilizes cells and stretches chromosomes extracted from the immobilized cells for a sample-to-analysis system in clinical diagnosis.
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Report
(4 results)
Research Products
(53 results)
