• Search Research Projects
  • Search Researchers
  • How to Use
  1. Back to previous page

Development of high throughput image cytometry for microalgal screening toward oil production

Research Project

Project/Area Number 23360363
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Research Field Biofunction/Bioprocess
Research InstitutionTokyo University of Agriculture and Technology

Principal Investigator

TANAKA Tsuyoshi  東京農工大学, 工学(系)研究科(研究院), 准教授 (20345333)

Co-Investigator(Kenkyū-buntansha) FUKUDA Yorikane  東京農工大学, 大学院・工学府, 特任助教 (40435855)
Project Period (FY) 2011-11-18 – 2014-03-31
Project Status Completed (Fiscal Year 2013)
Budget Amount *help
¥9,230,000 (Direct Cost: ¥7,100,000、Indirect Cost: ¥2,130,000)
Fiscal Year 2013: ¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000)
Fiscal Year 2012: ¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000)
Keywords高速イメージサイトメトリー / 微細藻類 / スクリーニング / バイオ燃料 / 微細孔アレイ / スクリーニング技術 / レンズレスイメージング / CMOSセンサー / 中性脂質 / 突然変異株スクリーニング / 単一細胞モニタリング / 脂肪酸組成分析
Research Abstract

High throughput image cytometry was developed for screening of microalgae with high oil production. The microcavity-culture device created large scale cell patterning, and then time-lapse imaging was performed to observe cell growth and oil accumulation. Additionally, colony formation monitoring system was also developed using a CMOS image sensor. It improved observation throughput, which allowed us to overcome current limitation of the microalgal screening. The image cytometry system developed in this study is expected to be used to explore promising microalgal producers of biofuel, as well as other variable materials including carotenoid, glycosphingolipid and polyunsaturated lipid.

Report

(4 results)
  • 2013 Annual Research Report   Final Research Report ( PDF )
  • 2012 Annual Research Report
  • 2011 Annual Research Report
  • Research Products

    (46 results)

All 2014 2013 2012 Other

All Journal Article (16 results) (of which Peer Reviewed: 6 results) Presentation (24 results) Remarks (2 results) Patent(Industrial Property Rights) (4 results)

  • [Journal Article] Monitoring of Cellular Behaviors by Microcavity Array-Based Single-Cell Patterning2014

    • Author(s)
      Kyoko Osada, Masahito Hosokawa, Tomoko Yoshino, Tsuyoshi Tanaka
    • Journal Title

      Analyst

      Volume: 139 Issue: 2 Pages: 425-430

    • DOI

      10.1039/c3an01698f

    • Related Report
      2013 Annual Research Report 2013 Final Research Report
    • Peer Reviewed
  • [Journal Article] Digital cell counting device integrated With a single-cell array2014

    • Author(s)
      佐伯 達也
    • Journal Title

      PLOS ONE

      Volume: 9 Issue: 2 Pages: e89011-e89011

    • DOI

      10.1371/journal.pone.0089011

    • Related Report
      2013 Annual Research Report 2013 Final Research Report
    • Peer Reviewed
  • [Journal Article] Establishment of a Genetic Transformation System for the Marine Pennate Diatom Fistulifera sp. Strain JPCC DA0580 - a High Triglyceride Producer2013

    • Author(s)
      Masaki Muto, Yorikane Fukuda, Michiko Nemoto, Tomoko Yoshino, Tadashi Matsunaga, Tsuyoshi Tanaka
    • Journal Title

      Marine Biotech.

      Volume: 15 Issue: 1 Pages: 48-55

    • DOI

      10.1007/s10126-012-9457-0

    • Related Report
      2013 Final Research Report
    • Peer Reviewed
  • [Journal Article] A Process Design and Productivity Evaluation for Oil Production by Indoor Mass Cultivation of a Marine Diatom, Fistulifera sp. JPCC DA05802013

    • Author(s)
      Akira Satoh, Kyonosuke Ichii, Mitsufumi Matsumoto, Chihiro Kubota, Michiko Nemoto, Masayoshi Tanaka, Tomoko Yoshino, Tadashi Matsunaga, Tsuyoshi Tanaka
    • Journal Title

      Bioresource Technology

      Volume: 137 Pages: 132-138

    • DOI

      10.1016/j.biortech.2013.03.087

    • Related Report
      2013 Final Research Report 2012 Annual Research Report
    • Peer Reviewed
  • [Journal Article] 2030 年への挑戦 "藻類を燃料に"2013

    • Author(s)
      田中剛
    • Journal Title

      太陽エネルギー

      Volume: vol.39 Pages: 27-35

    • NAID

      10031146196

    • Related Report
      2013 Final Research Report
  • [Journal Article] 海洋バイオマスからのバイオディーゼル燃料生産2013

    • Author(s)
      田中剛
    • Journal Title

      化学と生物

      Volume: vol.51 Pages: 183-188

    • NAID

      10031156789

    • Related Report
      2013 Final Research Report
  • [Journal Article] Establishment of a Genetic Transformation System for The Marine Pennate Diatom Fistulifera sp. JPCC DA0580 - a High Triglyceride Producer2013

    • Author(s)
      武藤 正記
    • Journal Title

      Marine Biotechnology

      Volume: Vol. 15, No. 1 Pages: 48-55

    • Related Report
      2012 Annual Research Report
    • Peer Reviewed
  • [Journal Article] 2030年への挑戦“藻類を燃料に”2013

    • Author(s)
      田中 剛
    • Journal Title

      太陽エネルギー

      Volume: 39 Pages: 27-35

    • NAID

      10031146196

    • Related Report
      2012 Annual Research Report
  • [Journal Article] 海洋バイオマスからのバイオディーゼル燃料生産2013

    • Author(s)
      田中 剛
    • Journal Title

      化学と生物

      Volume: 51 Pages: 183-188

    • NAID

      10031156789

    • Related Report
      2012 Annual Research Report
  • [Journal Article] 藻類から得られるバイオ燃料の可能性と今後の課題2012

    • Author(s)
      田中剛
    • Journal Title

      未来材料

      Volume: vol.12, No.3 Pages: 37-43

    • Related Report
      2013 Final Research Report 2011 Annual Research Report
  • [Journal Article] 海洋珪藻の次世代バイオ燃料への応用2012

    • Author(s)
      田中剛
    • Journal Title

      生物工学

      Volume: vol.90 Pages: 392-395

    • NAID

      110009480335

    • Related Report
      2013 Final Research Report
  • [Journal Article] 海洋微細藻類を用いた液体燃料生産技術の可能性と今後の展開2012

    • Author(s)
      田中剛
    • Journal Title

      Journal of the Japanese Institute of Energy

      Volume: vol.91 Pages: 1161-1165

    • NAID

      110009560463

    • Related Report
      2013 Final Research Report
  • [Journal Article] 海洋珪藻の次世代バイオ燃料への応用2012

    • Author(s)
      田中 剛
    • Journal Title

      生物工学

      Volume: 90 Pages: 392-395

    • NAID

      110009480335

    • Related Report
      2012 Annual Research Report
  • [Journal Article] 海洋微細藻類を用いた液体燃料生産技術の可能性と今後の展開2012

    • Author(s)
      田中 剛
    • Journal Title

      Journal of the Japanese Institute of Energy

      Volume: 91 Pages: 1161-1165

    • NAID

      110009560463

    • Related Report
      2012 Annual Research Report
  • [Journal Article] 藻類から得られるバイオ燃料の可能性と今後の課題2012

    • Author(s)
      田中 剛
    • Journal Title

      未来材料

      Volume: Vol. 12, No. 3 Pages: 37-43

    • Related Report
      2012 Annual Research Report
  • [Journal Article] Establishment of a genetic transformation system for the marine pennate diatom Fistulifera sp. strain JPCC DA0580-a high triglyceride producer2012

    • Author(s)
      Masaki Muto
    • Journal Title

      Marine Biotechnology

      Volume: (in press)

    • Related Report
      2011 Annual Research Report
    • Peer Reviewed
  • [Presentation] 顕微ラマン分光法による海洋珪藻 Fistilifera sp. JPCC DA0580 株の脂質解析2013

    • Author(s)
      向井将一朗, 田中剛
    • Organizer
      第65回日本生物工学会大会
    • Place of Presentation
      広島国際会議場(広島県)
    • Related Report
      2013 Final Research Report
  • [Presentation] Microcavity array を用いた shadow imaging に基づく細胞カウンターの開発2013

    • Author(s)
      佐伯達也, 田中剛
    • Organizer
      2013年電気化学秋季年会
    • Place of Presentation
      東京工業大学大岡山キャンパス(東京都)
    • Related Report
      2013 Final Research Report
  • [Presentation] 単一細胞パターニングによる海洋珪藻 Fistulifera sp. JPCC DA0580株のオイル蓄積過程のタイムラプス解析2013

    • Author(s)
      長田響子, 田中剛
    • Organizer
      第15回マリンバイオテクノロジー学会大会
    • Place of Presentation
      沖縄県市町村自治会館(沖縄県)
    • Related Report
      2013 Final Research Report
  • [Presentation] 顕微ラマン分光法による海洋珪藻 Fistulifera sp. JPCC DA0580 株の脂質イメージング解析2013

    • Author(s)
      吉野知子, 田中剛
    • Organizer
      顕微ラマン分光法による海洋珪藻 Fistulifera sp. JPCC DA0580 株の脂質イメージング解析
    • Place of Presentation
      沖縄県市町村自治会館(沖縄県)
    • Related Report
      2013 Final Research Report
  • [Presentation] In vivo lipid analysis of marine diatom Fistulifera sp. strain JPCC DA0580 using Raman Microspectroscopy2013

    • Author(s)
      向井将一朗, 田中剛
    • Organizer
      The First Taiwan International Symposium on Raman Spectroscopy
    • Place of Presentation
      National Chiao Tung University, Taiwan
    • Related Report
      2013 Final Research Report
  • [Presentation] 高オイル生産海洋珪藻Fistulifera sp. JPCC DA0580 株における delta 9 desaturase 遺伝子の機能解析2013

    • Author(s)
      武藤正記, 田中剛
    • Organizer
      日本化学会第93春季大会
    • Place of Presentation
      立命館大学びわこ・くさつキャンパス(滋賀県)
    • Related Report
      2013 Final Research Report
  • [Presentation] Multiple Omics Analysis of the Oleaginous Marine Diatom for Oil Production2013

    • Author(s)
      田中剛
    • Organizer
      Innovative Bio-Production of Fuels Chemicals from Renewable
    • Place of Presentation
      Breakthrough Theastrette, Biopolis, Singapore
    • Related Report
      2013 Final Research Report 2012 Annual Research Report
  • [Presentation] 単一細胞パターニングによる海洋珪藻Fistulifera sp. JPCC DA0580株のオイル蓄積過程のタイムラプス解析2013

    • Author(s)
      長田響子
    • Organizer
      第15回マリンバイオテクノロジー学会大会
    • Place of Presentation
      沖縄県市町村自治会館(沖縄県)
    • Related Report
      2013 Annual Research Report
  • [Presentation] 顕微ラマン分光法による海洋珪藻Fistulifera sp. JPCC DA0580株の脂質イメージング解析2013

    • Author(s)
      吉野知子
    • Organizer
      第15回マリンバイオテクノロジー学会大会
    • Place of Presentation
      沖縄県市町村自治会館(沖縄県)
    • Related Report
      2013 Annual Research Report
  • [Presentation] In vivo lipid analysis of marine diatom Fistulifera sp. strain JPCC DA0580 using Raman Microspectroscopy2013

    • Author(s)
      Shoichiro Mukai
    • Organizer
      The First Taiwan International Symposium on Raman Spectroscopy (2013)
    • Place of Presentation
      National Chiao Tung University (Taiwan)
    • Related Report
      2013 Annual Research Report
  • [Presentation] Microcavity arrayを用いたshadow imagingに基づく細胞カウンターの開発2013

    • Author(s)
      佐伯達也
    • Organizer
      2013年電気化学秋季大会
    • Place of Presentation
      東京工業大学大岡山キャンパス(東京都)
    • Related Report
      2013 Annual Research Report
  • [Presentation] 顕微ラマン分光法による海洋珪藻Fistulifera sp. JPCC DA0580株の脂質解析2013

    • Author(s)
      向井将一朗
    • Organizer
      第65回日本生物工学会大会
    • Place of Presentation
      広島国際会議場(広島県)
    • Related Report
      2013 Annual Research Report
  • [Presentation] 高オイル生産海洋珪藻Fistulifera sp. JPCC DA0580株におけるdelta 9 desaturase遺伝子の機能解析2013

    • Author(s)
      武藤 正記
    • Organizer
      日本化学会第93春季大会
    • Place of Presentation
      立命館大学びわこ・くさつキャンパス、滋賀
    • Related Report
      2012 Annual Research Report
  • [Presentation] RNA-seq に基づく海洋珪藻 Fistulifera sp. solaris 株のオイル蓄積機構の解析2012

    • Author(s)
      根本理子, 田中剛
    • Organizer
      日本化学会第92春季年会
    • Place of Presentation
      慶應義塾大学日吉キャンパス(神奈川県)
    • Year and Date
      2012-03-28
    • Related Report
      2013 Final Research Report
  • [Presentation] 細胞集積化基板を用いた浮遊細胞のタイムラプス解析2012

    • Author(s)
      長田響子, 田中剛
    • Organizer
      日本化学会第92春季年会
    • Place of Presentation
      慶應義塾大学日吉キャンパス(神奈川県)
    • Year and Date
      2012-03-28
    • Related Report
      2013 Final Research Report
  • [Presentation] RNA-seqに基づく海洋珪藻Fistulifera sp.solaris株のオイル蓄積機構の解析2012

    • Author(s)
      根本理子
    • Organizer
      日本化学会第92春季年会(2012)
    • Place of Presentation
      慶應義塾大学日吉キャンパス(神奈川県)
    • Year and Date
      2012-03-28
    • Related Report
      2011 Annual Research Report
  • [Presentation] 細胞集積化基板を用いた浮遊細胞のタイムラプス解析2012

    • Author(s)
      長田響子
    • Organizer
      日本化学会第92春季年会(2012)
    • Place of Presentation
      慶應義塾大学日吉キャンパス(神奈川県)
    • Year and Date
      2012-03-28
    • Related Report
      2011 Annual Research Report
  • [Presentation] Microcavity Array-Based Living Cell Patterning for Tracking Cellular Behavior at the Single-Cell Level2012

    • Author(s)
      長田響子, 田中剛
    • Organizer
      International Joint Symposium on Single-Cell Analysis
    • Place of Presentation
      Kyoto Research Park, Kyoto, Japan
    • Related Report
      2013 Final Research Report
  • [Presentation] Isolation and Functional Analysis of Novel Desaturases Involved in Triglyceride Biosynthesis from Oleaginous Marine Diatom Fistulifera sp. Strain JPCC DA05802012

    • Author(s)
      久保田千尋, 田中剛
    • Organizer
      The 9th Asia-Pacific Marine Biotechnology Conference
    • Place of Presentation
      Kochi City Cultural Praza "CUL-PORT", Kochi, Japan
    • Related Report
      2013 Final Research Report
  • [Presentation] Establishment of Gene Transformation Technique of Marine Pennate Diatom Fistulifera sp. strain JPCC DA0580 for Effective Biodiesel Production2012

    • Author(s)
      武藤正記, 田中剛
    • Organizer
      112th General Meeting American Society for Microbiology
    • Place of Presentation
      Moscone center, San Francisco, USA
    • Related Report
      2013 Final Research Report
  • [Presentation] Transcriptomic Analysis of The Oleaginous Marine Diatom Fistulifera sp. JPCC DA0580 toward Biodiesel production2012

    • Author(s)
      田中剛
    • Organizer
      The 2nd International Conference on Algal Biomass, Biofuels and Bioproducts
    • Place of Presentation
      The Weatin Gaslamp Quarter, San Diego, USA
    • Related Report
      2013 Final Research Report 2012 Annual Research Report
  • [Presentation] Establishment of Gene Transformation Technique of Marine Pennate Diatom Fistulifera sp. strain JPCC DA0580 for Effective Biodiesel Production2012

    • Author(s)
      武藤 正記
    • Organizer
      112th General Meeting American Society for Microbiology
    • Place of Presentation
      Moscone center,San Francisco, USA
    • Related Report
      2012 Annual Research Report
  • [Presentation] Isolation and Functional Analysis of Novel Desaturases Involved in Triglyceride Biosynthesis from Oleaginous Marine Diatom Fistuliufera sp. Strain JPCC DA05802012

    • Author(s)
      久保田 千尋
    • Organizer
      The 9th Asia-Pacific Marine Biotechnology Conference
    • Place of Presentation
      Kochi City Cultural Plaza “CUL-PORT”, Kochi, Japan
    • Related Report
      2012 Annual Research Report
  • [Presentation] Microcavity Array-Based Living Cell Patterning for Tracking Cellular Behavior at the Single-Cell Level2012

    • Author(s)
      長田 響子
    • Organizer
      International Joint Symposium on Single-Cell Analysis
    • Place of Presentation
      Kyoto Research park
    • Related Report
      2012 Annual Research Report
  • [Remarks]

    • URL

      http://www.tuat.ac.jp/~biomol/

    • Related Report
      2013 Final Research Report
  • [Remarks]

    • URL

      http://www.tuat.ac.jp/~biomol/publication6.html

    • Related Report
      2011 Annual Research Report
  • [Patent(Industrial Property Rights)] 細胞解析方法及び装置2014

    • Inventor(s)
      田中剛,松永是,佐伯達也,原田学,林泰圭
    • Industrial Property Rights Holder
      国立大学法人東京農工大学,株式会社マルコム
    • Industrial Property Rights Type
      特許
    • Filing Date
      2014-02-20
    • Related Report
      2013 Final Research Report
  • [Patent(Industrial Property Rights)] 細胞解析方法及び装置2014

    • Inventor(s)
      田中剛,松永是,佐伯達也,原田学,林泰圭
    • Industrial Property Rights Holder
      国立大学法人東京農工大学,株式会社マルコム
    • Industrial Property Rights Type
      特許
    • Filing Date
      2014-02-20
    • Related Report
      2013 Final Research Report
  • [Patent(Industrial Property Rights)] 細胞解析方法及び装置2014

    • Inventor(s)
      田中剛、松永是、佐伯達也、原田学、林泰圭
    • Industrial Property Rights Holder
      国立大学法人東京農工大学、株式会社マルコム
    • Industrial Property Rights Type
      特許
    • Industrial Property Number
      2014-030547
    • Filing Date
      2014-02-20
    • Related Report
      2013 Annual Research Report
  • [Patent(Industrial Property Rights)] 細胞解析方法及び装置2014

    • Inventor(s)
      田中剛、松永是、佐伯達也、原田学、林泰圭
    • Industrial Property Rights Holder
      国立大学法人東京農工大学、株式会社マルコム
    • Industrial Property Rights Type
      特許
    • Industrial Property Number
      2014-030550
    • Filing Date
      2014-02-20
    • Related Report
      2013 Annual Research Report

URL: 

Published: 2013-05-15   Modified: 2019-07-29  

Information User Guide FAQ News Terms of Use Attribution of KAKENHI

Powered by NII kakenhi