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Construction of protein materials which regulate cellular functions for regenerative mediceine

Research Project

Project/Area Number 23360364
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Research Field Biofunction/Bioprocess
Research InstitutionTokyo Institute of Technology

Principal Investigator

KOBATAKE Eiry  東京工業大学, 総合理工学研究科(研究院), 教授 (00225484)

Project Period (FY) 2011-04-01 – 2015-03-31
Project Status Completed (Fiscal Year 2014)
Budget Amount *help
¥18,200,000 (Direct Cost: ¥14,000,000、Indirect Cost: ¥4,200,000)
Fiscal Year 2013: ¥6,110,000 (Direct Cost: ¥4,700,000、Indirect Cost: ¥1,410,000)
Fiscal Year 2012: ¥6,370,000 (Direct Cost: ¥4,900,000、Indirect Cost: ¥1,470,000)
Fiscal Year 2011: ¥5,720,000 (Direct Cost: ¥4,400,000、Indirect Cost: ¥1,320,000)
Keywords生物機能工学 / 再生医工学 / 細胞機能制御 / タンパク質材料 / 組織構築 / 生体機能工学
Outline of Final Research Achievements

In this study, protein materials which can control neural cell differentiation were constructed by integration of newly designed scaffold and signal proteins. These proteins were assembled on the substrate, and neural stem cells were cultured on the surface. Cells were differentiated to neural cells and the resulting neural tissue was maintained its neural function stably.
The basis of protein materials for regenerative medicine for neural tissue was established through this study.

Report

(4 results)
  • 2014 Final Research Report ( PDF )
  • 2013 Annual Research Report
  • 2012 Annual Research Report
  • 2011 Annual Research Report
  • Research Products

    (24 results)

All 2014 2013 2012 2011

All Journal Article (12 results) (of which Peer Reviewed: 8 results,  Acknowledgement Compliant: 1 results) Presentation (12 results) (of which Invited: 1 results)

  • [Journal Article] Construction of bFGF-tethered multi-functional extracellular matrix protein through coiled-coil structures for neurite outgrowth induction2014

    • Author(s)
      M.Mie,S.Sasaki,E.Kobatake
    • Journal Title

      Biomed.Mater.

      Volume: 9 Issue: 1 Pages: 1-8

    • DOI

      10.1088/1748-6041/9/1/015004

    • Related Report
      2013 Annual Research Report
    • Peer Reviewed / Acknowledgement Compliant
  • [Journal Article] In vitro selection of peptide aptamers using a ribosome display for a conducting polymer2014

    • Author(s)
      Zha Li, Takanori Uzawa, Haichao Zhao, Shyh-Chyang Luo, Hsiao-hua Yu, Eiry Kobatake, and Yoshihiro Ito
    • Journal Title

      J. Biosci. Bioeng.

      Volume: 117 Issue: 4 Pages: 501-503

    • DOI

      10.1016/j.jbiosc.2013.10.005

    • NAID

      110009817067

    • Related Report
      2013 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Selection of DNA aptamers with affinity for pro-gastrin-releasing peptide (proGRP), a tumor marker for small cell lung cancer2013

    • Author(s)
      Mie M, Kai T, Le T, Cass AE, Kobatake E
    • Journal Title

      Appl Biochem Biotechnol.

      Volume: 169 Issue: 8 Pages: 250-255

    • DOI

      10.1007/s12010-012-9928-9

    • Related Report
      2012 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Construction of affinity changeable antibody in response to Ca2+2012

    • Author(s)
      Eiry Kobatake, Chihiro Kosaku, Satoshi Hanzawa, Masayasu Mie
    • Journal Title

      Biotechnol. Lett.

      Volume: 34 Issue: 6 Pages: 1019-1023

    • DOI

      10.1007/s10529-012-0881-z

    • Related Report
      2012 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Detection of antigens using a protein-DNA chimera developed by enzymatic covalent bonding with phiX gene A*2012

    • Author(s)
      Akter F, Mie M, Grimm S, Nygren PA, Kobatake E
    • Journal Title

      Anal Chem.

      Volume: 84(11) Issue: 11 Pages: 5040-5046

    • DOI

      10.1021/ac300708r

    • Related Report
      2012 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Induction of motor neuron differentiation by transduction of Olig2 protein2012

    • Author(s)
      Masayasu Mie, Mami Kaneko, Fumiaki Henmi, Eiry Kobatake
    • Journal Title

      Biochem. Biophys. Res. Commun

      Volume: 427(3) Issue: 3 Pages: 531-536

    • DOI

      10.1016/j.bbrc.2012.09.090

    • Related Report
      2012 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Construction of multi-functional extracellular matrix proteins that inhibits migration and tube formation of endothelial cells2011

    • Author(s)
      Nakamura M, Mie M, Nakamura M, Kobatake E
    • Journal Title

      Biotechnol Lett,

      Volume: 34(8) Issue: 8 Pages: 1571-7

    • DOI

      10.1007/s10529-011-0788-0

    • Related Report
      2012 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Construction of a bFGF-tethered extracellular matrix using a coiled-coil helical interaction2011

    • Author(s)
      Kobatake E.
    • Journal Title

      Bioconjugate Chem.

      Volume: 22 Issue: 10 Pages: 2038-2042

    • DOI

      10.1021/bc200249u

    • Related Report
      2011 Annual Research Report
  • [Journal Article] Immuno-rolling circle amplification using a multibinding fusion protein2011

    • Author(s)
      Akter F.
    • Journal Title

      Anal. Biochem.

      Volume: 15 Issue: 2 Pages: 174-179

    • DOI

      10.1016/j.ab.2011.05.004

    • Related Report
      2011 Annual Research Report
  • [Journal Article] Detection of small RNA molecules by a combination of branched rolling circle amplification and bioluminescent pyrophosphate assay2011

    • Author(s)
      Mashimo Y.
    • Journal Title

      Anal. Bioanal. Chem.

      Volume: 401 Issue: 1 Pages: 21-227

    • DOI

      10.1007/s00216-011-5083-3

    • Related Report
      2011 Annual Research Report
  • [Journal Article] Homeostasis of extracellular ATP in human erythrocytes2011

    • Author(s)
      Montalbetti N.
    • Journal Title

      J. Biol. Chem.

      Volume: 286 Issue: 44 Pages: 38397-38407

    • DOI

      10.1074/jbc.m111.221713

    • Related Report
      2011 Annual Research Report
  • [Journal Article] Direct detection of RNAs in living cells using peptide-inserted Renilla luciferase2011

    • Author(s)
      Takashi Andou
    • Journal Title

      Analyst

      Volume: 136 Issue: 12 Pages: 2446-2449

    • DOI

      10.1039/c1an15130d

    • Related Report
      2011 Annual Research Report
    • Peer Reviewed
  • [Presentation] 骨組織再生を目的とした新規細胞外マトリックスの構築2014

    • Author(s)
      弘田裕介、三重正和、小畠英理
    • Organizer
      第36回日本バイオマテリアル学会大会
    • Place of Presentation
      船堀(東京都江戸川区)
    • Year and Date
      2014-11-17 – 2014-11-18
    • Related Report
      2013 Annual Research Report
  • [Presentation] Construction of multifunctional proteins by integration of scaffolds and growth factors2014

    • Author(s)
      E.Kobatake,M.Mie
    • Organizer
      ESB2014
    • Place of Presentation
      Liverpool (England)
    • Year and Date
      2014-08-31 – 2014-09-03
    • Related Report
      2013 Annual Research Report
  • [Presentation] 神経組織再生のためのCNTF融合ECMの構築2013

    • Author(s)
      金載憲、三重正和、小畠英理
    • Organizer
      日本化学会第93春季年会
    • Place of Presentation
      立命館大学びわこ・くさつキャンパス
    • Related Report
      2012 Annual Research Report
  • [Presentation] Construction of the ECM-growth factor integrated protein through ciled-coil structure to promote angiogenesis2012

    • Author(s)
      Yasmine Assal, Masayasu Mie, Eiry Kobatake
    • Organizer
      TERMIS World Congress 2012
    • Place of Presentation
      Viena
    • Related Report
      2012 Annual Research Report
  • [Presentation] Phi X174 Gene A*タンパク質を用いたDNA-タンパク質融合分子作製法の開発とその応用2012

    • Author(s)
      真下泰正、鈴木繁哉、三重正和、小畠英理
    • Organizer
      第6回バイオ関連化学シンポジウム
    • Place of Presentation
      北海道大学、札幌
    • Related Report
      2012 Annual Research Report
  • [Presentation] Consruction of multifunctional protein materials for tissue engineering2012

    • Author(s)
      Eiry Kobatake
    • Organizer
      ISABE 2012
    • Place of Presentation
      Guilin, China
    • Related Report
      2012 Annual Research Report
    • Invited
  • [Presentation] Construction and application of semisynthetic DNA-protein conjugates with PhiX174 gene A* protein2012

    • Author(s)
      Yasumasa Mashimo, Shigeya Suzuki, Masayasu Mie, Eiry Kobatake
    • Organizer
      ISABE 2012
    • Place of Presentation
      Guilin, China
    • Related Report
      2012 Annual Research Report
  • [Presentation] 成長因子と自己組織化ペプチドを融合したマトリクスタンパク質の構築2011

    • Author(s)
      大室繭
    • Organizer
      第33回日本バイオマテリアル学会大会
    • Place of Presentation
      京都(京都テルサ)
    • Related Report
      2011 Annual Research Report
  • [Presentation] 組織特異的転写因子タンパク質導入による細胞分化制御2011

    • Author(s)
      金子真美
    • Organizer
      第33回日本バオイオマテリアル学会大会
    • Place of Presentation
      京都(京都テルサ)
    • Related Report
      2011 Annual Research Report
  • [Presentation] Split-SNAP tagを利用した新規分子イメージング法の開発2011

    • Author(s)
      三重正和
    • Organizer
      第5回バイオ関連化学シンポジウム
    • Place of Presentation
      筑波(エポカル筑波)
    • Related Report
      2011 Annual Research Report
  • [Presentation] PhiX174A*タンパク質を用いたDNA-タンパク質融合分子作製法の開発2011

    • Author(s)
      真下泰正
    • Organizer
      2011年電気化学秋季大会
    • Place of Presentation
      新潟(朱鷺メッセ)
    • Related Report
      2011 Annual Research Report
  • [Presentation] AN NOVEL ENZYMATIC METHOD FOR SITE-SPECIFIC LABELING OF RECOMBINANT PROTEINS WITH DNA2011

    • Author(s)
      Yasumasa Mashimo
    • Organizer
      Nature Chemical Biology Symposium 2011
    • Place of Presentation
      Boston(Royal Sonesta Hotel Boston)
    • Related Report
      2011 Annual Research Report

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Published: 2011-04-06   Modified: 2019-07-29  

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