| Budget Amount *help |
¥14,040,000 (Direct Cost: ¥10,800,000、Indirect Cost: ¥3,240,000)
Fiscal Year 2013: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2012: ¥5,330,000 (Direct Cost: ¥4,100,000、Indirect Cost: ¥1,230,000)
Fiscal Year 2011: ¥6,890,000 (Direct Cost: ¥5,300,000、Indirect Cost: ¥1,590,000)
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| Research Abstract |
In this research, novel cell-based biosensing system with a high resolution of two dimensional surface plasmon resonance (2D-SPR) imager was developed. Response of some neural or endocrine cells which were adhered on an Au chip to agonist stimulation was successfully observed by the reflection intensity change in the individual cell regions with the 2D-SPR imager at real-time. It was further demonstrated that the cell response in 2D-SPR imaging to agonist stimulation might be based on intracellular recruitment of signal transduction proteins such as PKC to cell membrane.
Furthermore, we have found that the electro-orientation of cells such as cylinder-like cells such as fission yeast under high frequency AC electric field clearly depended on the cell viability.
These results suggested future wide applications of label-free cell-based biosensing with 2D-SPR imaging method or electro-orientation observation method to drug screening, cell state evaluation, cell recognition and so on.
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