Development and application of Giga-screening system of by-molecules using cell-free biochemical reactions
| Project/Area Number |
23360367
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Biofunction/Bioprocess
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| Research Institution | Nagoya University |
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Principal Investigator |
NAKANO Hideo 名古屋大学, 生命農学研究科, 教授 (00237348)
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| Co-Investigator(Kenkyū-buntansha) |
IWASAKI Yugo 名古屋大学, 大学院生命農学研究科, 准教授 (50273214)
KOJIMA Takaaki 名古屋大学, 大学院生命農学研究科, 助教 (40909080)
KOBAYASHI Isao 独立行政法人農業・食品産業技術総合研究機構, 食品総合研究所, 研究員 (70425552)
兒島 孝明 名古屋大学, 生命農学研究科, 助教 (40509080)
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| Project Period (FY) |
2011-04-01 – 2015-03-31
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| Project Status |
Completed (Fiscal Year 2014)
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| Budget Amount *help |
¥18,330,000 (Direct Cost: ¥14,100,000、Indirect Cost: ¥4,230,000)
Fiscal Year 2014: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
Fiscal Year 2013: ¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000)
Fiscal Year 2012: ¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000)
Fiscal Year 2011: ¥8,190,000 (Direct Cost: ¥6,300,000、Indirect Cost: ¥1,890,000)
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| Keywords | 無細胞タンパク質合成系 / エマルジョン / タンパク質 / 酸化還元酵素 / RNAポリメラーゼ / 一分子PCR / マイクロビーズ / 酵素 / ハイスループットスクリーニング / ペルオキシダーゼ / スクリーニング / エマルジョンPCR / タンパク質工学 / セルラーゼ |
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| Outline of Final Research Achievements |
Creation of bio-macromolecules such as proteins, DNA and RNA is important technology both for bio-industries and bioscience, however, functional design of these molecules are generally impossible due to these complexities. In this research, we have developed a high-throughput library construction and screening system enabling giga-scale screening of such molecules using pL volume droplets in emulsion as reaction chamber for these molecules based on biochemical functions such as manganese peroxidase, horseradish peroxidase, and the substrate peptides and promoter sequences of transglutaminase and RNA polymerase, respectively.
In addition, hand-made device for making uniform droplets in emulsion formation in a short-time has been also developed to improve the screening efficiency.
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Report
(5 results)
Research Products
(28 results)
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[Journal Article] Comprehensive Analysis of the DNA-Binding Specificity of an <i>Aspergillus nidulans</i> Transcription Factor, AmyR, Using a Bead Display System2012
Author(s)
Wang, P., Kojima, T., Kobayashi, T., Nakano, H.
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Journal Title
Bioscience, Biotechnology, and Biochemistry
Volume: 76
Issue: 6
Pages: 1128-1134
DOI
NAID
ISSN
0916-8451, 1347-6947
Related Report
Peer Reviewed
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[Presentation] Cell-free synthesis of horseradish peroxidase and manganese peroxidase for bead display-based high-throughput screening.2013
Author(s)
Zhu, B., Ninomiya, R., Kojima, T., Iwasaki, Y., Nakano, H.
Organizer
日本農芸化学会中部支部第168回例会,
Place of Presentation
名古屋
Related Report
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