Application of protein cationization techniques for cancer immunotherapy
| Project/Area Number |
23360370
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Biofunction/Bioprocess
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| Research Institution | Okayama University |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
KAKIMI Kazuhiro 東京大学, 医学部付属病院, 准教授 (80273358)
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| Project Period (FY) |
2011-04-01 – 2014-03-31
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| Project Status |
Completed (Fiscal Year 2013)
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| Budget Amount *help |
¥18,330,000 (Direct Cost: ¥14,100,000、Indirect Cost: ¥4,230,000)
Fiscal Year 2013: ¥5,720,000 (Direct Cost: ¥4,400,000、Indirect Cost: ¥1,320,000)
Fiscal Year 2012: ¥5,720,000 (Direct Cost: ¥4,400,000、Indirect Cost: ¥1,320,000)
Fiscal Year 2011: ¥6,890,000 (Direct Cost: ¥5,300,000、Indirect Cost: ¥1,590,000)
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| Keywords | タンパク質工学 / 化学修飾 / バイオテクノロジー / 腫瘍免疫 / 診断薬 / ワクチン / 蛋白質工学 / がん免疫 / CT抗原 / 蛋白質生産 / タンパク質化学 / タンパク質生産 / タンパク質 / タンパク質精製 / 細胞内導入 |
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| Research Abstract |
This study aimed development of chemical protein cationization techniques for cancer antigens. Since most of cancer antigen proteins are flexible and unstable, solubilization with protein cationization techniques were useful methodology to prepare water-soluble and full-length cancer antigens. Using these cationized antigens, antigen antibodies for cancer antigens raised in cancer patients were successfully detected with a highly sensitive assay. Combining these developed techniques and enhanced cancer antigen production resource, we have successfully developed tools for cancer immunotherapy.
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Report
(4 results)
Research Products
(47 results)
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[Journal Article] Dramatic Increase in Expression of a Transgene by Insertion of Promoters Downstream of the Cargo Gene2014
Author(s)
Masakiyo Sakaguchi, Masami Watanabe, Rie Kinoshita, Haruki Kaku, Hideo Ueki, Junichiro Futami, Hitoshi Murata, Yusuke Inoue, Shun-Ai Li, Peng Huang, Endy Widya Putranto, I. Made, Winarsa Ruma, Yasutomo Nasu, Hiromi Kumon, Nam-ho Huh
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Journal Title
Mol. Biotechnol
Volume: 56
Issue: 7
Pages: 621-630
DOI
Related Report
Peer Reviewed
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