| Project/Area Number |
23370016
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Plant molecular biology/Plant physiology
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| Research Institution | Tokyo University of Agriculture and Technology |
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Principal Investigator |
OZEKI Yoshihiro 東京農工大学, 工学(系)研究科(研究院), 教授 (50185592)
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| Co-Investigator(Kenkyū-buntansha) |
OHTA Daisaki 大阪府立大学, 生命環境科学研究科, 教授 (10305659)
SASAKI Nobuhiro 東京農工大学, 大学院・工学研究院, 助教 (80422088)
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| Co-Investigator(Renkei-kenkyūsha) |
YOHDA Masahumi 東京農工大学, 大学院・工学研究院, 教授 (50250105)
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| Project Period (FY) |
2011-04-01 – 2014-03-31
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| Project Status |
Completed (Fiscal Year 2013)
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| Budget Amount *help |
¥18,330,000 (Direct Cost: ¥14,100,000、Indirect Cost: ¥4,230,000)
Fiscal Year 2013: ¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000)
Fiscal Year 2012: ¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000)
Fiscal Year 2011: ¥8,840,000 (Direct Cost: ¥6,800,000、Indirect Cost: ¥2,040,000)
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| Keywords | アントシアニン / 配糖化酵素 / アシル化酵素 / 液胞 / Zwitter donor / p-hydroxybenzyl-glucose / デルフィニウム / Delphinium grandiflorum / シロイヌナズナ / アシルグルコース / glycoside hydrolase family 1 / シネラリア / アガパンサス / アシル-グルコース |
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| Research Abstract |
Acyl-glucose dependent glucosyltransfrase (AAGT) gene was found in a monocot plant, Agapanthus, and Arabidopsis AAGT acted to acyl-modified anthocyanin with glucosyl residues in red turning leaves, indicating that AAGT reaction is common in the plant kingdom. In delphinium sepals, p-hydroxybenzyl-glucose (pHBG) acted as a glucosyl-donor at modification at the 7 position of anthocyanidin. The anthocyanin, violdelphin, consisted of three glycosyl and two p-hydroxybenzyl (pHBA)-residues, conjugated with alternately tandem and linear conjugation of glycosyl and pHBA-residues. In the reaction to form violdelphin, it was revealed that pHBG played a bi-functional role to be acyl donors and glucosyl donors for acyl-glucose dependent acyl-transferase and AAGT as Zwitter donor. The gene for UDP-glucose dependent pHBA glucosyltransferase was identified and the loss of this activity resulted not to modify at the 7 position of anthocyanidin.
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