| Budget Amount *help |
¥17,550,000 (Direct Cost: ¥13,500,000、Indirect Cost: ¥4,050,000)
Fiscal Year 2014: ¥3,380,000 (Direct Cost: ¥2,600,000、Indirect Cost: ¥780,000)
Fiscal Year 2013: ¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2012: ¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000)
Fiscal Year 2011: ¥6,110,000 (Direct Cost: ¥4,700,000、Indirect Cost: ¥1,410,000)
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| Outline of Final Research Achievements |
Exine, a rigid and chemically stable architecture on the surface of pollen grains, serves a protector of male gametes. We identified many Arabidopsis kaonashi mutants that produce pollen grains with abnormal exine structure. Twelve responsible genes of the mutants determined in this study revealed that a certain class of polysaccharides plays important roles for exine construction. Pollen coat consisting mainly of proteins and lipids is another surface component of pollen grains. Biosynthesis and requirement of two major proteins, EXL and GRP, in Arabidopsis pollen coat were analyzed. We also visualized the process of pollen coat formation in anthers and its behavior on the surface of stigmatic papilla cells.
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