Analysis of auxin-induced activation of the plasma membrane H+-ATPase
| Project/Area Number |
23370019
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Plant molecular biology/Plant physiology
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| Research Institution | Nagoya University |
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Principal Investigator |
KINOSHITA Toshinori 名古屋大学, トランスフォーマティブ生命分子研究所(理), 教授 (50271101)
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| Co-Investigator(Renkei-kenkyūsha) |
TAKAHASHI Koji 名古屋大学, 大学院・理学研究科, 助教 (40283379)
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| Project Period (FY) |
2011-04-01 – 2014-03-31
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| Project Status |
Completed (Fiscal Year 2013)
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| Budget Amount *help |
¥16,380,000 (Direct Cost: ¥12,600,000、Indirect Cost: ¥3,780,000)
Fiscal Year 2013: ¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2012: ¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000)
Fiscal Year 2011: ¥8,450,000 (Direct Cost: ¥6,500,000、Indirect Cost: ¥1,950,000)
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| Keywords | オーキシン / 細胞膜プロトンポンプ / オーキシン受容体 / 胚軸伸長 / 細胞膜H+-ATPase / リン酸化 / 細胞伸長 / 黄化芽生え / シロイヌナズナ |
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| Research Abstract |
In this study, we showed that auxin induces phosphorylation of the penultimate Thr in the plasma membrane H+-ATPase without involvement of the identified auxin receptors, TIR1/AFB, in Arabidopsis etiolated seedlings, and that auxin-induced phosphorylation is required for the early phase of auxin-induced hypocotyl elongation. Now, we are trying to identify the unidentified auxin receptor by reverse genetic and biochemical approaches. In addition, we have started to screen the mutants, which show reduced auxin-induced hypocotyl elongation and phosphorylation of the plasma membrane H+-ATPase in EMS-treated Arabidopsis etiolated seedlings.
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Report
(4 results)
Research Products
(127 results)
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[Journal Article] TWIN SISTER OF FT, GIGANTEA, and CONSTANS have a positive but indirect effect on blue light-induced stomatal opening in Arabidopsisthahana.2013
Author(s)
Ando E, Ohnishi M, Wang Y, Matsushita T, Watanabe A, Hayashi Y, Fujii M, Ma JF, Inoue S, Kinoshita T
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Journal Title
Plant Physiology
Volume: 162
Issue: 3
Pages: 1529-38
DOI
Related Report
Peer Reviewed / Open Access
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[Journal Article] FLOWERING LOCUS T regulates stomatal opening2011
Author(s)
Kinoshita T, Ono N, Hayashi Y, Morimoto S, Nakamura S, Soda M, Kato Y, Ohnishi M, Nakano T, Inoue S, Shimazaki K
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Journal Title
Current Biology
Volume: 21
Issue: 14
Pages: 1232-1238
DOI
Related Report
Peer Reviewed
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