Dynamic analyses of subcellular structures using pinpoint functional depletion

• Project/Area Number: 23370029
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Morphology/Structure
• Research Institution: Tokyo University of Science
• Principal Investigator: MATSUNAGA Sachihiro 東京理科大学, 理工学部, 准教授 (40323448)
• Co-Investigator(Kenkyū-buntansha): KUTSUNA Natsumaro 東京大学, 大学院・新領域創成科学研究科, 助教 (70578559)
• Project Period (FY): 2011-04-01 – 2014-03-31
• Project Status: Completed (Fiscal Year 2013)
• Budget Amount: ¥17,940,000 (Direct Cost: ¥13,800,000、Indirect Cost: ¥4,140,000); Fiscal Year 2013: ¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000); Fiscal Year 2012: ¥5,720,000 (Direct Cost: ¥4,400,000、Indirect Cost: ¥1,320,000); Fiscal Year 2011: ¥7,280,000 (Direct Cost: ¥5,600,000、Indirect Cost: ¥1,680,000)
• Keywords: 細胞分裂 / 染色体 / 顕微鏡 / イメージング / 光分子不活性化法 / 光活性化タンパク質 / ピンポイント / 機能阻害 / 細胞動態 / 微細構造 / KillerRed / 光阻害 / ライブセルイメージング / ROS / タンパク質

Research Abstract

Dynamic analyses of subcellular structures using pinpoint functional depletion
The purpose of this project is to reveal the dynamics of cell division in animals and plants using pinpoint functional depletion. The pinpoint functional depletion using a chromosomal protein RBMX fused with a photosensitizer protein was successfully performed by high-power laser. Spatiotemporal inactivation of RBMX with the photosensitizer was performed in combination with live cell imaging. At the result, RBMX interaction with chromatin is turned out through the spatiotemporal inactivation. This method to induce the delay of cell division can be performed through generation of reactive oxygen species and structural destruction of the protein complex by laser irradiation to fluorescent protein-labelled subcellular structures.

Research Products

• [Journal Article] Chromophore-assisted laser inactivation - towards a spatiotemporal-functional analysis of proteins, and the ablation of chromatin, organelle and cell function (2014)
  • Author(s): Sano, Y., Watanabe, W. and Matsunaga, S.
  • Journal Title: JOURNAL OF CELL SCIENCE Volume: 127 Issue: 8 Pages: 1621-1629
  • DOI: 10.1242/jcs.144527
  • Peer Reviewed / Open Access
• [Journal Article] The kineain-like protein TOP promotes Aurora localisation and induces mitochondrial, chloroplast and nuclear division (2013)
  • Author(s): Yamato Yoshida, Takayuki Fujiwara, Yuuta Imoto, Masaki Yoshida, Mio Ohnuma, Shunsuke Hirooka, Osami Misumi, Haruko Kuroiwa, Tsuneyoshi Kuroiwa
  • Journal Title: Journal of Cell Science Volume: 126 Pages: 2392-2400
  • DOI: 10.1242/jcs.116798
  • Peer Reviewed
• [Journal Article] New insights into the dynamics of plant cell nuclei and chromosomes (2013)
  • Author(s): Matsunaga, S., Katagiri, Y., Nagashima, Y., Sugiyama, T., Hasegawa, J., Hayashi, K. and Sakamoto, T.
  • Journal Title: INTERNATIONAL REVIEW OF CELL AND MOLECULAR BIOLOGY Volume: 305 Pages: 253-301
  • DOI: 10.1016/b978-0-12-407695-2.00006-8
  • Peer Reviewed
• [Journal Article] Cytokinins control endocycle onset by promoting the expression of an APC/C activator in Arabidopsis roots (2013)
  • Author(s): Takahashi, N., Kajihara, T., Okamura, C., Kim, Y., Katagiri, Y., Okushima, Y., Matsunaga, S., Hwang, I. and Umeda, M.
  • Journal Title: Curr. Biol. Volume: 23 Issue: 18 Pages: 1812-1817
  • DOI: 10.1016/j.cub.2013.07.051
  • NAID: 120005716839
  • Peer Reviewed
• [Journal Article] The boundary of the meristematic and elongation zones in roots : endoreduplication precedes rapid cell expansion (2013)
  • Author(s): Kohma Hayashi, Junko Hasegawa, Sachihiro Matsunaga
  • Journal Title: Scientific Reports Volume: 3 Issue: 1 Pages: 2723-2723
  • DOI: 10.1038/srep02723
  • Peer Reviewed
• [Journal Article] ASURA (Phb2) interacts with Sccl through chromatin. (2013)
  • Author(s): Equilibrina, I.
  • Journal Title: Cytogenet. Genome Res Volume: (in press) Issue: 4 Pages: 225-233
  • DOI: 10.1159/000350004
  • Peer Reviewed
• [Journal Article] Application of visualization techniques for cell and tissue engineering. (2013)
  • Author(s): Higashi, T.
  • Journal Title: J. Boisci. Bioeng. Volume: 115 Issue: 2 Pages: 122-126
  • DOI: 10.1016/j.jbiosc.2012.09.008
  • Peer Reviewed
• [Journal Article] RBMX: a regulator for maintenance and centromeric protection of sister chromatid cohesion. (2012)
  • Author(s): Matsunaga, S.
  • Journal Title: Cell Rep. Volume: 19 Issue: 4 Pages: 299-308
  • DOI: 10.1016/j.celrep.2012.02.005
  • Peer Reviewed
• [Journal Article] Active learning framework with iterative clustering for bioimage classification. (2012)
  • Author(s): Kutsuna, N.
  • Journal Title: Nature Commun. Volume: 3 Issue: 1 Pages: 1032-1032
  • DOI: 10.1038/ncomms2030
  • Peer Reviewed
• [Journal Article] GIGAS CELL1, a novel negative regulator of the anaphase- promoting complex/cyclosome, is required for proper mitotic progression and cell fate determination in Arabidopsis thaliana. (2011)
  • Author(s): Iwata, E., Ikeda S., Mtsunaga, S., Kurata, M., Yoshioka, Y., Criqui, M.-C., Genschik, P., Ito, M
  • Journal Title: The Plant Cell Volume: vol. 23 (12) Issue: 12 Pages: 4382-4393
  • DOI: 10.1105/tpc.111.092049
  • Peer Reviewed
• [Journal Article] Assembly states of the nucleosome assembly protein 1 (NAP-1) revealed by sedimentation velocity and non-denaturing MS (2011)
  • Author(s): Noda M, Uchiyama S, McKay AR, Morimoto A, Misawa S, Yoshida A, Shimahara H, Takinowaki H, Nakamura S, Kobayashi Y, Matsunaga S, Ohkubo T, Robinson CV, Fukui K
  • Journal Title: Biochem J. Volume: 436 Issue: 1 Pages: 101-112
  • DOI: 10.1042/bj20102063
  • Peer Reviewed
• [Journal Article] ASURA (PHB2) Is Required for Kinetochore Assembly and Subsequent Chromosome Congression (2011)
  • Author(s): Lee, M. H., et al
  • Journal Title: ACTA HISTOCHEMICA ET CYTOCHEMICA Volume: 44 Issue: 6 Pages: 247-258
  • DOI: 10.1267/ahc.11033
  • NAID: 130001258489
  • ISSN: 0044-5991, 1347-5800
  • Peer Reviewed
• [Presentation] Imaging analyses of DNA replication and cell division i n root devel opment (2014)
  • Author(s): Sachihiro Matsunaga
  • Organizer: 第55回日本植物生理学会年会
  • Place of Presentation: 富山大学 五福キャンパス
  • Year and Date: 2014-03-18
• [Presentation] Subnuclear dynam ics and cell dynamics in root morphogen esis (2013)
  • Author(s): Sachihiro Matsunaga
  • Organizer: Workshop on Modeling Biomolecula r Systems in Cellular Environment
  • Place of Presentation: 京都大学理学研究科セミナーハウス
  • Year and Date: 2013-10-31
  • Invited
• [Presentation] Correlation between DN A content and cell volume under DNA dam age and auxi n starvati on (2013)
  • Author(s): Junko Hasegawa, Takuya Sakamoto, Sach ihiro Matsunaga
  • Organizer: 第54回日本植物生理学会年会
  • Place of Presentation: 岡山大学津島キャンパス
  • Year and Date: 2013-03-21
• [Presentation] Dynamics analyse s of RBMX responsible for chromosome co hesion and bioimage classification by C ARTA (2013)
  • Author(s): Sachihiro Matsunaga
  • Organizer: 4-th France-Japan Joint Seminar
  • Place of Presentation: RIKEN Harima I nstitute / Spring-8 / SACLA
  • Year and Date: 2013-01-09
• [Presentation] 植物の動原体局在型オーロラキナーゼは微小管プラス末端制御タンパク質EB1をリン酸化する (2013)
  • Author(s): 松永幸大, 北原英里奈, 栗原大輔, 小牧伸一郎, 高木麻衣, 佐野行己, 長島慶宜, 中神弘史, 伊藤正樹, 杉本慶子, 橋本隆, 松永朋子, 坂本卓也
  • Organizer: 第36回日本分子生物学会年会
  • Place of Presentation: 神戸ポートアイランド
• [Presentation] ライブセルイメージングによる細胞動態解析 (2012)
  • Author(s): 松永幸大
  • Organizer: 日本女子大学バイオイメージングセンター第4回公開シンポジウム
  • Place of Presentation: 日本女子大学八十年館
  • Year and Date: 2012-10-27
  • Invited
• [Presentation] DNA複製とゲノム倍加 (2012)
  • Author(s): 松永幸大
  • Organizer: 日本植物学会第76回大会
  • Place of Presentation: 兵庫県立大学姫路書写キャンパス
  • Year and Date: 2012-09-17
• [Presentation] 生物画像の自動分類ソフトウェア「カルタ」の開発 (2012)
  • Author(s): 松永幸大, 朽名夏麿, 桧垣匠, 馳澤盛一郎
  • Organizer: 日本植物形態学会第24回大会
  • Place of Presentation: 兵庫県立大学姫路書写キャンパス
  • Year and Date: 2012-09-14
• [Presentation] 染色体接着に関与するタンパク質RBMXの解析 (2011)
  • Author(s): 松永幸大
  • Organizer: 第63回日本細胞生物学会大会
  • Place of Presentation: 北海道大学クラーク会館, 学術交流会館
• [Presentation] 染色体接着に関与するタンパク質RBMXの解析 (2011)
  • Author(s): 松永幸大
  • Organizer: 第63回日本細胞生物学会大会
  • Place of Presentation: 北海道大学クラーク会館、学術交流会館
• [Presentation] Imaging analyses of DNA replication and cell division in root development
  • Author(s): Sachihiro Matsunaga
  • Organizer: 第55回日本植物生理学会年会
  • Place of Presentation: 富山大学 五福キャンパス
• [Presentation] Plant Aurora kinase localizes at the centromeric region and phosphorylates microtubule-related proteins
  • Author(s): 松永幸大、北原英里奈、栗原大輔、小牧伸一郎、高木麻衣、佐野行己、長島慶宜、中神弘史、伊藤正樹、杉本慶子、橋本隆、松永朋子、坂本卓也
  • Organizer: 第36回日本分子生物学会年会
  • Place of Presentation: 神戸ポートアイランド
• [Presentation] Subnuclear dynamics and cell dynamics in root morphogenesis.
  • Author(s): Sachihiro Matsunaga
  • Organizer: Workshop on Modeling Biomolecular Systems in Cellular Environment
  • Place of Presentation: 京都大学理学研究科セミナーハウス
  • Invited
• [Presentation] Dynamics analyses of RBMX responsible for chromosome cohesion and bioimage classification by CARTA
  • Author(s): Matsunaga, S.
  • Organizer: 4-th France-Japan Joint Seminar
  • Place of Presentation: RIKEN Harima Institute / Spring-8 / SACLA
  • Invited
• [Presentation] Correlation between DNA content and cell volume under DNA damage and auxin starvation
  • Author(s): Matsunaga, S.
  • Organizer: 第54回日本植物生理学会年会
  • Place of Presentation: 岡山大学津島キャンパス
• [Presentation] DNA複製とゲノム倍加
  • Author(s): 松永幸大
  • Organizer: 日本植物学会第76回大会
  • Place of Presentation: 兵庫県立大学 姫路書写キャンパス
• [Presentation] 生物画像の自動分類ソフトウェア「カルタ」の開発
  • Author(s): 松永幸大
  • Organizer: 日本植物形態学会第24回大会
  • Place of Presentation: 兵庫県立大学 姫路書写キャンパス
• [Book] Atlas of Plant Cell Structure (2014)
  • Author(s): Tetsuko Noguchi, Shigeyuki Kawano, Atsushi Sakai, Yasuko Hayashi, Ichiro Karahara, Sachihiro Matsunaga and Hirokazu Tsukaya
  • Publisher: Springer
• [Book] The role of repetitive sequences in plant sex chromosome evolution. "New Insights on Plant Sex Chromosomes" edited by Navajas R (2012)
  • Author(s): Kazama, Y. and Matsunaga, S
  • Publisher: Nova Scientific Publisher (New York, USA)
• [Book] The organisation of genomic DNA in mitotic chromosomes: a novel view. "Molecular Biology and Evolution of the Plant Genome" edited by Greilhuber, J. , Leitch, I. , Wendel, J. and Dolezel, J. (2012)
  • Author(s): Takata, H. , Matsunaga, S. and Maeshima, K.
  • Publisher: Springer Verlag (Heidelberg, Germany)
• [Book] New Insights on Plant Sex Chromosomes (2012)
  • Author(s): Kazama, Y.
  • Publisher: Nova Scientific Publisher
• [Book] Molecular Biology and Evolution of the Plant Genome (2012)
  • Author(s): Takata, H.
  • Publisher: Springer Verlag
• [Remarks]
  • URL: http://www.rs.tus.ac.jp/sachi/
• [Remarks] 研究情報データベースRIDAI
  • URL: https://www.tus.ac.jp/ridai/doc/ji/RIJIA01User.php?act=nam&kin=pap&diu=64d9
• [Remarks] 松永研究室
  • URL: http://www.rs.tus.ac.jp/sachi/
• [Remarks]
  • URL: http://www.rs.tus.ac.jp/sachi/