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Dynamic analyses of subcellular structures using pinpoint functional depletion

Research Project

Project/Area Number 23370029
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Research Field Morphology/Structure
Research InstitutionTokyo University of Science

Principal Investigator

MATSUNAGA Sachihiro  東京理科大学, 理工学部, 准教授 (40323448)

Co-Investigator(Kenkyū-buntansha) KUTSUNA Natsumaro  東京大学, 大学院・新領域創成科学研究科, 助教 (70578559)
Project Period (FY) 2011-04-01 – 2014-03-31
Project Status Completed (Fiscal Year 2013)
Budget Amount *help
¥17,940,000 (Direct Cost: ¥13,800,000、Indirect Cost: ¥4,140,000)
Fiscal Year 2013: ¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000)
Fiscal Year 2012: ¥5,720,000 (Direct Cost: ¥4,400,000、Indirect Cost: ¥1,320,000)
Fiscal Year 2011: ¥7,280,000 (Direct Cost: ¥5,600,000、Indirect Cost: ¥1,680,000)
Keywords細胞分裂 / 染色体 / 顕微鏡 / イメージング / 光分子不活性化法 / 光活性化タンパク質 / ピンポイント / 機能阻害 / 細胞動態 / 微細構造 / KillerRed / 光阻害 / ライブセルイメージング / ROS / タンパク質
Research Abstract

Dynamic analyses of subcellular structures using pinpoint functional depletion
The purpose of this project is to reveal the dynamics of cell division in animals and plants using pinpoint functional depletion. The pinpoint functional depletion using a chromosomal protein RBMX fused with a photosensitizer protein was successfully performed by high-power laser. Spatiotemporal inactivation of RBMX with the photosensitizer was performed in combination with live cell imaging. At the result, RBMX interaction with chromatin is turned out through the spatiotemporal inactivation. This method to induce the delay of cell division can be performed through generation of reactive oxygen species and structural destruction of the protein complex by laser irradiation to fluorescent protein-labelled subcellular structures.

Report

(4 results)
  • 2013 Annual Research Report   Final Research Report ( PDF )
  • 2012 Annual Research Report
  • 2011 Annual Research Report
  • Research Products

    (38 results)

All 2014 2013 2012 2011 Other

All Journal Article (12 results) (of which Peer Reviewed: 12 results,  Open Access: 1 results) Presentation (17 results) (of which Invited: 4 results) Book (5 results) Remarks (4 results)

  • [Journal Article] Chromophore-assisted laser inactivation - towards a spatiotemporal-functional analysis of proteins, and the ablation of chromatin, organelle and cell function2014

    • Author(s)
      Sano, Y., Watanabe, W. and Matsunaga, S.
    • Journal Title

      JOURNAL OF CELL SCIENCE

      Volume: 127 Issue: 8 Pages: 1621-1629

    • DOI

      10.1242/jcs.144527

    • Related Report
      2013 Annual Research Report 2013 Final Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] The kineain-like protein TOP promotes Aurora localisation and induces mitochondrial, chloroplast and nuclear division2013

    • Author(s)
      Yamato Yoshida, Takayuki Fujiwara, Yuuta Imoto, Masaki Yoshida, Mio Ohnuma, Shunsuke Hirooka, Osami Misumi, Haruko Kuroiwa, Tsuneyoshi Kuroiwa
    • Journal Title

      Journal of Cell Science

      Volume: 126 Pages: 2392-2400

    • DOI

      10.1242/jcs.116798

    • Related Report
      2013 Annual Research Report 2013 Final Research Report 2012 Annual Research Report
    • Peer Reviewed
  • [Journal Article] New insights into the dynamics of plant cell nuclei and chromosomes2013

    • Author(s)
      Matsunaga, S., Katagiri, Y., Nagashima, Y., Sugiyama, T., Hasegawa, J., Hayashi, K. and Sakamoto, T.
    • Journal Title

      INTERNATIONAL REVIEW OF CELL AND MOLECULAR BIOLOGY

      Volume: 305 Pages: 253-301

    • DOI

      10.1016/b978-0-12-407695-2.00006-8

    • Related Report
      2013 Annual Research Report 2013 Final Research Report
    • Peer Reviewed
  • [Journal Article] Cytokinins control endocycle onset by promoting the expression of an APC/C activator in Arabidopsis roots2013

    • Author(s)
      Takahashi, N., Kajihara, T., Okamura, C., Kim, Y., Katagiri, Y., Okushima, Y., Matsunaga, S., Hwang, I. and Umeda, M.
    • Journal Title

      Curr. Biol.

      Volume: 23 Issue: 18 Pages: 1812-1817

    • DOI

      10.1016/j.cub.2013.07.051

    • NAID

      120005716839

    • Related Report
      2013 Annual Research Report 2013 Final Research Report
    • Peer Reviewed
  • [Journal Article] The boundary of the meristematic and elongation zones in roots : endoreduplication precedes rapid cell expansion2013

    • Author(s)
      Kohma Hayashi, Junko Hasegawa, Sachihiro Matsunaga
    • Journal Title

      Scientific Reports

      Volume: 3 Issue: 1 Pages: 2723-2723

    • DOI

      10.1038/srep02723

    • Related Report
      2013 Annual Research Report 2013 Final Research Report
    • Peer Reviewed
  • [Journal Article] ASURA (Phb2) interacts with Sccl through chromatin.2013

    • Author(s)
      Equilibrina, I.
    • Journal Title

      Cytogenet. Genome Res

      Volume: (in press) Issue: 4 Pages: 225-233

    • DOI

      10.1159/000350004

    • Related Report
      2013 Final Research Report 2012 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Application of visualization techniques for cell and tissue engineering.2013

    • Author(s)
      Higashi, T.
    • Journal Title

      J. Boisci. Bioeng.

      Volume: 115 Issue: 2 Pages: 122-126

    • DOI

      10.1016/j.jbiosc.2012.09.008

    • Related Report
      2013 Final Research Report 2012 Annual Research Report
    • Peer Reviewed
  • [Journal Article] RBMX: a regulator for maintenance and centromeric protection of sister chromatid cohesion.2012

    • Author(s)
      Matsunaga, S.
    • Journal Title

      Cell Rep.

      Volume: 19 Issue: 4 Pages: 299-308

    • DOI

      10.1016/j.celrep.2012.02.005

    • Related Report
      2013 Final Research Report 2012 Annual Research Report 2011 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Active learning framework with iterative clustering for bioimage classification.2012

    • Author(s)
      Kutsuna, N.
    • Journal Title

      Nature Commun.

      Volume: 3 Issue: 1 Pages: 1032-1032

    • DOI

      10.1038/ncomms2030

    • Related Report
      2013 Final Research Report 2012 Annual Research Report
    • Peer Reviewed
  • [Journal Article] GIGAS CELL1, a novel negative regulator of the anaphase- promoting complex/cyclosome, is required for proper mitotic progression and cell fate determination in Arabidopsis thaliana.2011

    • Author(s)
      Iwata, E., Ikeda S., Mtsunaga, S., Kurata, M., Yoshioka, Y., Criqui, M.-C., Genschik, P., Ito, M
    • Journal Title

      The Plant Cell

      Volume: vol. 23 (12) Issue: 12 Pages: 4382-4393

    • DOI

      10.1105/tpc.111.092049

    • Related Report
      2013 Final Research Report 2012 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Assembly states of the nucleosome assembly protein 1 (NAP-1) revealed by sedimentation velocity and non-denaturing MS2011

    • Author(s)
      Noda M, Uchiyama S, McKay AR, Morimoto A, Misawa S, Yoshida A, Shimahara H, Takinowaki H, Nakamura S, Kobayashi Y, Matsunaga S, Ohkubo T, Robinson CV, Fukui K
    • Journal Title

      Biochem J.

      Volume: 436 Issue: 1 Pages: 101-112

    • DOI

      10.1042/bj20102063

    • Related Report
      2013 Final Research Report 2011 Annual Research Report
    • Peer Reviewed
  • [Journal Article] ASURA (PHB2) Is Required for Kinetochore Assembly and Subsequent Chromosome Congression2011

    • Author(s)
      Lee, M. H., et al
    • Journal Title

      ACTA HISTOCHEMICA ET CYTOCHEMICA

      Volume: 44 Issue: 6 Pages: 247-258

    • DOI

      10.1267/ahc.11033

    • NAID

      130001258489

    • ISSN
      0044-5991, 1347-5800
    • Related Report
      2013 Final Research Report 2011 Annual Research Report
    • Peer Reviewed
  • [Presentation] Imaging analyses of DNA replication and cell division i n root devel opment2014

    • Author(s)
      Sachihiro Matsunaga
    • Organizer
      第55回日本植物生理学会年会
    • Place of Presentation
      富山大学 五福キャンパス
    • Year and Date
      2014-03-18
    • Related Report
      2013 Final Research Report
  • [Presentation] Subnuclear dynam ics and cell dynamics in root morphogen esis2013

    • Author(s)
      Sachihiro Matsunaga
    • Organizer
      Workshop on Modeling Biomolecula r Systems in Cellular Environment
    • Place of Presentation
      京都大学理学研究科セミナーハウス
    • Year and Date
      2013-10-31
    • Related Report
      2013 Final Research Report
    • Invited
  • [Presentation] Correlation between DN A content and cell volume under DNA dam age and auxi n starvati on2013

    • Author(s)
      Junko Hasegawa, Takuya Sakamoto, Sach ihiro Matsunaga
    • Organizer
      第54回日本植物生理学会年会
    • Place of Presentation
      岡山大学津島キャンパス
    • Year and Date
      2013-03-21
    • Related Report
      2013 Final Research Report
  • [Presentation] Dynamics analyse s of RBMX responsible for chromosome co hesion and bioimage classification by C ARTA2013

    • Author(s)
      Sachihiro Matsunaga
    • Organizer
      4-th France-Japan Joint Seminar
    • Place of Presentation
      RIKEN Harima I nstitute / Spring-8 / SACLA
    • Year and Date
      2013-01-09
    • Related Report
      2013 Final Research Report
  • [Presentation] 植物の動原体局在型オーロラキナーゼは微小管プラス末端制御タンパク質EB1をリン酸化する2013

    • Author(s)
      松永幸大, 北原英里奈, 栗原大輔, 小牧伸一郎, 高木麻衣, 佐野行己, 長島慶宜, 中神弘史, 伊藤正樹, 杉本慶子, 橋本隆, 松永朋子, 坂本卓也
    • Organizer
      第36回日本分子生物学会年会
    • Place of Presentation
      神戸ポートアイランド
    • Related Report
      2013 Final Research Report
  • [Presentation] ライブセルイメージングによる細胞動態解析2012

    • Author(s)
      松永幸大
    • Organizer
      日本女子大学バイオイメージングセンター第4回公開シンポジウム
    • Place of Presentation
      日本女子大学八十年館
    • Year and Date
      2012-10-27
    • Related Report
      2013 Final Research Report 2012 Annual Research Report
    • Invited
  • [Presentation] DNA複製とゲノム倍加2012

    • Author(s)
      松永幸大
    • Organizer
      日本植物学会第76回大会
    • Place of Presentation
      兵庫県立大学姫路書写キャンパス
    • Year and Date
      2012-09-17
    • Related Report
      2013 Final Research Report
  • [Presentation] 生物画像の自動分類ソフトウェア「カルタ」の開発2012

    • Author(s)
      松永幸大, 朽名夏麿, 桧垣匠, 馳澤盛一郎
    • Organizer
      日本植物形態学会第24回大会
    • Place of Presentation
      兵庫県立大学姫路書写キャンパス
    • Year and Date
      2012-09-14
    • Related Report
      2013 Final Research Report
  • [Presentation] 染色体接着に関与するタンパク質RBMXの解析2011

    • Author(s)
      松永幸大
    • Organizer
      第63回日本細胞生物学会大会
    • Place of Presentation
      北海道大学クラーク会館, 学術交流会館
    • Related Report
      2013 Final Research Report
  • [Presentation] 染色体接着に関与するタンパク質RBMXの解析2011

    • Author(s)
      松永幸大
    • Organizer
      第63回日本細胞生物学会大会
    • Place of Presentation
      北海道大学クラーク会館、学術交流会館
    • Related Report
      2011 Annual Research Report
  • [Presentation] Imaging analyses of DNA replication and cell division in root development

    • Author(s)
      Sachihiro Matsunaga
    • Organizer
      第55回日本植物生理学会年会
    • Place of Presentation
      富山大学 五福キャンパス
    • Related Report
      2013 Annual Research Report
  • [Presentation] Plant Aurora kinase localizes at the centromeric region and phosphorylates microtubule-related proteins

    • Author(s)
      松永幸大、北原英里奈、栗原大輔、小牧伸一郎、高木麻衣、佐野行己、長島慶宜、中神弘史、伊藤正樹、杉本慶子、橋本隆、松永朋子、坂本卓也
    • Organizer
      第36回日本分子生物学会年会
    • Place of Presentation
      神戸ポートアイランド
    • Related Report
      2013 Annual Research Report
  • [Presentation] Subnuclear dynamics and cell dynamics in root morphogenesis.

    • Author(s)
      Sachihiro Matsunaga
    • Organizer
      Workshop on Modeling Biomolecular Systems in Cellular Environment
    • Place of Presentation
      京都大学理学研究科セミナーハウス
    • Related Report
      2013 Annual Research Report
    • Invited
  • [Presentation] Dynamics analyses of RBMX responsible for chromosome cohesion and bioimage classification by CARTA

    • Author(s)
      Matsunaga, S.
    • Organizer
      4-th France-Japan Joint Seminar
    • Place of Presentation
      RIKEN Harima Institute / Spring-8 / SACLA
    • Related Report
      2012 Annual Research Report
    • Invited
  • [Presentation] Correlation between DNA content and cell volume under DNA damage and auxin starvation

    • Author(s)
      Matsunaga, S.
    • Organizer
      第54回日本植物生理学会年会
    • Place of Presentation
      岡山大学津島キャンパス
    • Related Report
      2012 Annual Research Report
  • [Presentation] DNA複製とゲノム倍加

    • Author(s)
      松永幸大
    • Organizer
      日本植物学会第76回大会
    • Place of Presentation
      兵庫県立大学 姫路書写キャンパス
    • Related Report
      2012 Annual Research Report
  • [Presentation] 生物画像の自動分類ソフトウェア「カルタ」の開発

    • Author(s)
      松永幸大
    • Organizer
      日本植物形態学会第24回大会
    • Place of Presentation
      兵庫県立大学 姫路書写キャンパス
    • Related Report
      2012 Annual Research Report
  • [Book] Atlas of Plant Cell Structure2014

    • Author(s)
      Tetsuko Noguchi, Shigeyuki Kawano, Atsushi Sakai, Yasuko Hayashi, Ichiro Karahara, Sachihiro Matsunaga and Hirokazu Tsukaya
    • Publisher
      Springer
    • Related Report
      2013 Annual Research Report 2013 Final Research Report
  • [Book] The role of repetitive sequences in plant sex chromosome evolution. "New Insights on Plant Sex Chromosomes" edited by Navajas R2012

    • Author(s)
      Kazama, Y. and Matsunaga, S
    • Publisher
      Nova Scientific Publisher (New York, USA)
    • Related Report
      2013 Final Research Report
  • [Book] The organisation of genomic DNA in mitotic chromosomes: a novel view. "Molecular Biology and Evolution of the Plant Genome" edited by Greilhuber, J. , Leitch, I. , Wendel, J. and Dolezel, J.2012

    • Author(s)
      Takata, H. , Matsunaga, S. and Maeshima, K.
    • Publisher
      Springer Verlag (Heidelberg, Germany)
    • Related Report
      2013 Final Research Report
  • [Book] New Insights on Plant Sex Chromosomes2012

    • Author(s)
      Kazama, Y.
    • Publisher
      Nova Scientific Publisher
    • Related Report
      2012 Annual Research Report
  • [Book] Molecular Biology and Evolution of the Plant Genome2012

    • Author(s)
      Takata, H.
    • Publisher
      Springer Verlag
    • Related Report
      2012 Annual Research Report
  • [Remarks]

    • URL

      http://www.rs.tus.ac.jp/sachi/

    • Related Report
      2013 Final Research Report
  • [Remarks] 研究情報データベースRIDAI

    • URL

      https://www.tus.ac.jp/ridai/doc/ji/RIJIA01User.php?act=nam&kin=pap&diu=64d9

    • Related Report
      2013 Annual Research Report
  • [Remarks] 松永研究室

    • URL

      http://www.rs.tus.ac.jp/sachi/

    • Related Report
      2012 Annual Research Report
  • [Remarks]

    • URL

      http://www.rs.tus.ac.jp/sachi/

    • Related Report
      2011 Annual Research Report

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Published: 2011-04-06   Modified: 2019-07-29  

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