| Budget Amount *help |
¥17,940,000 (Direct Cost: ¥13,800,000、Indirect Cost: ¥4,140,000)
Fiscal Year 2014: ¥3,510,000 (Direct Cost: ¥2,700,000、Indirect Cost: ¥810,000)
Fiscal Year 2013: ¥3,510,000 (Direct Cost: ¥2,700,000、Indirect Cost: ¥810,000)
Fiscal Year 2012: ¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000)
Fiscal Year 2011: ¥6,890,000 (Direct Cost: ¥5,300,000、Indirect Cost: ¥1,590,000)
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| Outline of Final Research Achievements |
We have already reported that cortisol is deeply involved in temperature-dependent sex determination. Here, we first showed that exposure to high temperature (HT) or cortisol after hatching inhibited the expression of fshr and cyp19a1 mRNAs, and induced the expression of gsdf mRNA in XX gonads during gonadal sex differentiation. Next, we analyzed the phenotypes of FSHR-knockout, CYP19A1-knockout and GSDF-overexpressed transgenic medaka. As results, among these medaka lines only GSDF-overexpressed XX medaka were completely sex-reversed into phenotypic males. Furthermore, quantitative real-time PCR analysis showed that the overexpression suppressed the expression of fshr and cyp19a1 mRNAs in XX gonads during gonadal sex differentiation. These results strongly suggest that HT induces masculinization of XX medaka by elevation of cortisol levels, which, in turn, causes suppression of fshr and cyp19a1 expression while inducing gsdf expression in XX gonads.
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