Analysis of the endocrine and neural mechanisms that synergistically regulate the behavior and physiology of the worker honeybees.

• Project/Area Number: 23370032
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Animal physiology/Animal behavior
• Research Institution: The University of Tokyo
• Principal Investigator: KUBO Takeo 東京大学, 理学(系)研究科(研究院), 教授 (10201469)
• Co-Investigator(Kenkyū-buntansha): TAKEUCHI Hideaki 東京大学, 大学院・理学系研究科, 助教 (00376534) ; KUNIEDA Takekazu 東京大学, 大学院・理学系研究科, 助教 (10463879)
• Project Period (FY): 2011-04-01 – 2014-03-31
• Project Status: Completed (Fiscal Year 2013)
• Budget Amount: ¥18,200,000 (Direct Cost: ¥14,000,000、Indirect Cost: ¥4,200,000); Fiscal Year 2013: ¥5,850,000 (Direct Cost: ¥4,500,000、Indirect Cost: ¥1,350,000); Fiscal Year 2012: ¥5,850,000 (Direct Cost: ¥4,500,000、Indirect Cost: ¥1,350,000); Fiscal Year 2011: ¥6,500,000 (Direct Cost: ¥5,000,000、Indirect Cost: ¥1,500,000)
• Keywords: ミツバチ / 脳 / キノコ体 / ケニヨン細胞 / 採餌行動 / 外分泌 / ローヤルゼリー / 細胞増殖因子 / mKast / 初期応答遺伝子 / 神経活動 / 航空術 / 社会性行動 / カルシウム情報伝達系 / 熱殺蜂球 / プロテオミクス / 外分泌腺 / エクダイソン / モジュール構造

Research Abstract

In this study, we aimed to unveil brain neural mechanisms and physiological mechanisms that underlie the honeybee social behaviors. Here, we unveiled novel cellular characteristics of the large-type Kenyon cells (KCs) and identified novel 'middle-type' KCs, both of which compose the mushroom bodies, a higher brain center. The neural activities of the small-type and some 'middle-type' KCs are enhanced in the foragers, whereas those of the class II KCs are enhanced in the Ja@anese worker honeybees that are forming a 'hot defensive bee ball' against the hornets. Furthermore, we showed that the royal jelly that is secreted from young workers (nurse bees) is a cocktail of secretory proteins derived from three exocrine glands and contains cell growth factors that may affect physiological status of the other nest mates.

Research Products

• [Journal Article] Identification and characterization of an Egr ortholog as a neural immediate early gene in the European honeybee (Apis mellifera L.) (2013)
  • Author(s): Atsushi Ugajin, Takekazu Kunieda, Takeo Kubo
  • Journal Title: FEBS Letters Volume: 587 Issue: 19 Pages: 3224-3230
  • DOI: 10.1016/j.febslet.2013.08.014
  • Peer Reviewed
• [Journal Article] Novel Middle-Type Kenyon Cells in the Honeybee Brain Revealed by Area-Preferential Gene Expression Analysis (2013)
  • Author(s): K Kaneko, T Ikeda, M Nagai, S Hori, C Umatani, H Tadano, A Ugajin, T Nakakoka, R K Paul, T Fujiyuki, Ki Shirai, T Kuniedal, Hideaki T, T Kubo
  • Journal Title: PLOS ONE Volume: 8 Issue: 8 Pages: e71732-e71732
  • DOI: 10.1371/journal.pone.0071732
  • Peer Reviewed
• [Journal Article] Proteomic analysis of the royal jelly and characterization of the functions of its derivation glands in the honeybee (2013)
  • Author(s): Fujita, T., Kozuka-Hata, H., Ao-Kondo, H., Kunieda, T., Oyama, M. and Kubo, T.
  • Journal Title: Journal of Proteome Research Volume: 12 Issue: 1 Pages: 404-411
  • DOI: 10.1021/pr300700e
  • Peer Reviewed
• [Journal Article] Identification of kakusei, a Nuclear Non-Coding RNA, as an Immediate Early Gepe fromthe Honeybee and Its Application for Neuroethological Study (2012)
  • Author(s): Taketoshi Kiya
  • Journal Title: International Journal of Molecular Sciences Volume: 13 Issue: 12 Pages: 15496-15509
  • DOI: 10.3390/ijms131215496
  • Peer Reviewed
• [Journal Article] Mushroom body-preferential expression of proteins/genes involved in endoplasmic reticulum Ca2+-transport in the worker honeybee (Apis mellifera L.) brain (2012)
  • Author(s): Uno, Y., Fujiyuki, T., Morioka, M and Kubo, T
  • Journal Title: Insect Mol.Biol Volume: 22(1) Issue: 1 Pages: 52-61
  • DOI: 10.1111/imb.12002
  • Peer Reviewed
• [Journal Article] Detection of neural activity in the brains of Japanese honeybee workers during the formation of a "hot defensive bee ball" (2012)
  • Author(s): Ugajin, A., Kiya, T., Kunieda, T., Ono, M., Yoshida, T. and Kubo, T
  • Journal Title: PLoS ONE Volume: 7(3)
• [Journal Article] Detection of neural activity in the brains of Japanese honeybee workers during the formation of a "hot defensive bee ball" (2012)
  • Author(s): Ugajin, A., Kiya, T., Kunieda, T., Ono, M., Yoshida, T., Kubo, T.
  • Journal Title: PLoS ONE Volume: 7(3) Issue: 3 Pages: e32902-e32902
  • DOI: 10.1371/journal.pone.0032902
  • Peer Reviewed
• [Journal Article] Ecdysteroid biosynthesis in workers of the European honeybee (Apis mellifera L.) (2011)
  • Author(s): Yamazaki, Y., Kiuchi, M., Takeuchi, H., Kubo, T.
  • Journal Title: Insect Biochem.Mol.Biol. Volume: 41 Issue: 5 Pages: 283-293
  • DOI: 10.1016/j.ibmb.2011.01.005
  • Peer Reviewed
• [Journal Article] Dance type and flight parameters are associated with different mushroom body neural activities in worker honeybee brains (2011)
  • Author(s): Kiya, T., Kubo, T.
  • Journal Title: PLoS ONE Volume: 6(4) Issue: 4 Pages: e19301-e19301
  • DOI: 10.1371/journal.pone.0019301
  • NAID: 120003141901
  • Peer Reviewed
• [Journal Article] Expression of two microRNAs, ame-mir-276 and -1000, in the adult honeybee (Apis mellifera L.) brain (2011)
  • Author(s): Hori, S., Kaneko, K., Saito, T.H., Takeuchi, H., Kubo, T.
  • Journal Title: Apidologie Volume: 42 Issue: 1 Pages: 89-102
  • DOI: 10.1051/apido/2010032
  • Peer Reviewed
• [Presentation] セイヨウミツバチにおけるEgr-1ホモログの同定と脊椎動物-昆虫間で保存された初期応答遺伝子としての性状解析 (2014)
  • Author(s): 宇賀神篤, 國枝武和, 久保健雄
  • Organizer: 第58回日本応用動物昆虫学会大会
  • Place of Presentation: 高知
• [Presentation] セイヨウミツバチにおけるEgr-1ホモログの同定と脊椎動物-昆虫間で保存された初期応答遺伝子としての性状解析 (2014)
  • Author(s): 宇賀神 篤、國枝 武和、久保 健雄
  • Organizer: 第58回日本応用動物昆虫学会大会
  • Place of Presentation: 高知大学朝倉キャンパス（高知県）
• [Presentation] Identification and characterization of an Egr ortholog in the European honeybee (Apis mellifera L.) as a conserved neural IEG in insects and vertebrates' (2013)
  • Author(s): Ugajin, A., Kunieda, T. and Kubo, T
  • Organizer: 第36回日本分子生物学会年会
  • Place of Presentation: 神戸
• [Presentation] Identification of three genes expressed highly selectively in the mushroom bodies in the European honeybee Apis mellifera L. brain (2013)
  • Author(s): 末次翔太, 竹内秀明, 國枝武和, 久保健雄
  • Organizer: 日本動物学会第84回岡山大会
  • Place of Presentation: 岡山大学津島キャンパス
• [Presentation] 初期応答遺伝子を利用したニホンミツバチとセイヨウミツバチにおける高温曝露時のキノコ体神経活動の解析 (2013)
  • Author(s): 宇賀神篤, 國枝武和, 小野正人, 久保健雄
  • Organizer: 第57回日本応用動物昆虫学会
  • Place of Presentation: 藤沢
• [Presentation] Identification and characterization of an Egr ortholog in the European honeybee (Apis mellifera L.) as a conserved neural IEG in insects and vertebrates (2013)
  • Author(s): Ugajin, A., Kunieda, T. and Takeo Kubo
  • Organizer: 第36回日本分子生物学会年
  • Place of Presentation: 神戸国際展示場（兵庫県）
• [Presentation] Identification of three genes expressed highly selectively in the mushroom bodies in the European honeybee Apis mellifera (2013)
  • Author(s): 末次翔太、竹内秀明、國枝武和、久保健雄
  • Organizer: 日本動物学会 第84回岡山大会
  • Place of Presentation: 岡山大学津島キャンパス（岡山県）
• [Presentation] 初期応答遺伝子を利用したニホンミツバチとセイヨウミツバチにおける高温曝露時のキノコ体神経活動の解析 (2013)
  • Author(s): 宇賀神篤、國枝武和、小野正人、吉田忠晴、久保健雄
  • Organizer: 日本昆虫学会第72回大会
  • Place of Presentation: 神奈川県藤沢市 日本大学
• [Presentation] Functional characterization of hypopharyngeal gland and salivary glands of the worker honeybees by using proteomics (2012)
  • Author(s): Fujita, T., Hata, K.-H., Kondo, H., Kunieda, T., Oyama, M. and Kubo, T
  • Organizer: 3rd International Symposium Frontiers in Agriculture Proteome Research, Contribution of proteomics technology in agricultural Sciences
  • Place of Presentation: Tsukuba, JAPAN
  • Year and Date: 2012-12-10
• [Presentation] ミツバチの社会性行動を生み出す脳と遺伝子の仕組み (2012)
  • Author(s): 久保健雄
  • Organizer: 第2回岐阜薬科大学機能性健康食品研究講演会
  • Place of Presentation: 岐阜市ホテルグランベール岐山
  • Year and Date: 2012-11-26
  • Invited
• [Presentation] Royal Jelly Proteome Analysis and Functional Characterization of Hypopharyngeal Gland and Salivary Glands of the Worker Honeybees by Using Proteomics (2012)
  • Author(s): Fujita, T.
  • Organizer: BIT's 1st Annual Conference and EXPO of AnalytiX-2012
  • Place of Presentation: Beijing, China
  • Year and Date: 2012-03-24
• [Presentation] ミツバチの社会性行動を生み出す脳と遺伝子の仕組み (2012)
  • Author(s): 久保健雄
  • Organizer: 第34回ミツバチ科学研究会玉川学園
  • Place of Presentation: 玉川大学
  • Year and Date: 2012-03-15
• [Presentation] ミツバチの社会性行動を生み出す脳と遺伝子の仕組み (2012)
  • Author(s): 久保健雄
  • Organizer: 第34回ミツバチ科学研究会
  • Place of Presentation: 玉川学園玉川大学(招待講演)
  • Year and Date: 2012-03-15
• [Presentation] 熱殺蜂球形成時のニホンミツバチ働き蜂の脳の神経興奮は高温情報処理を反映する (2012)
  • Author(s): 宇賀神篤、木矢剛智、國枝武和、小野正人、吉田忠晴、久保健雄
  • Organizer: 第56回日本応用動物昆虫学会大会
  • Place of Presentation: 奈良市近畿大学農学部
  • Year and Date: 2012-03-14
• [Presentation] セイヨウミツバチのローヤルゼリーとその由来外分泌腺のプロテオミクスを用いた解析 (2012)
  • Author(s): 藤田俊之, 秦裕子, 近藤裕子, 国枝武和, 尾山大明, 久保健雄
  • Organizer: 第85回日本生化学会大会
  • Place of Presentation: 福岡
• [Presentation] 熱殺蜂球形成時のニホンミツバチ脳内の神経興奮は高温情報処理を反映する (2012)
  • Author(s): 宇賀神篤, 木矢剛智, 國枝武和, 小野正人, 吉田忠晴, 久保健雄
  • Organizer: 日本昆虫学会第72回大会
  • Place of Presentation: 町田
• [Presentation] Neural activity in the brains of the Japanese worker honeybees involved in a hot defensive bee ball reflects thermal stimuli processing (2012)
  • Author(s): Ugajin, A., Kiya, T., Kunieda, T., Ono, M., Yoshida, T. and Kubo, T
  • Organizer: 10th International Congress of Neuroethology (ICN2012)
  • Place of Presentation: Maryland University
• [Presentation] Analysis of the royal jelly proteome and characterization of the functions of its derivation glands by using proteomics (2012)
  • Author(s): Fujita, T., Kozuka-Hata, H., Kondo, H., Kunieda, T., Oyama, M. and Kubo, T
  • Organizer: Meeting of The 45th Annual Meeting of the Japanese Society of Developmental Biologists & The 64th Annual Meeting of the Japan Society for Cell Biology
  • Place of Presentation: 神戸
• [Presentation] 熱殺蜂球形成時のニホンミ ツバチ働き蜂の脳の神経興奮は高温情報処理を反映する (2012)
  • Author(s): 宇賀神篤, 木矢剛智, 國枝武和, 小野正人, 吉田忠晴, 久保健雄
  • Organizer: 第56回日本応用 動物昆虫学会大会
  • Place of Presentation: 奈良
• [Presentation] Analysis of the royal jelly proteome and characterization of the functions of its derivation glands by using proteomics. (2012)
  • Author(s): Fujita, T., Hata, K.-H., Kondo, H., Kunieda, T., Oyama, M. and Kubo, T.
  • Organizer: Joint Meeting of The 45th Annual Meeting of the Japanese Society of Developmental Biologists & The 64th Annual Meeting of the Japan Society for Cell Biology.
  • Place of Presentation: 兵庫県神戸市 神戸国際会議場
• [Presentation] Neural activity in the brains of the Japanese worker honeybees involved in a hot defensive bee ball reflects thermal stimuli processing. (2012)
  • Author(s): Ugajin, A., Kunieda, T., Ono, M., Yoshida, T. and Kubo, T.
  • Organizer: Tenth International Congress of Neuroethology
  • Place of Presentation: University of Maryland, College Park, MD USA
• [Presentation] セイヨウミツバチのローヤルゼリーとその由来外分泌腺のプロテオミクスを用いた解析 (2012)
  • Author(s): 藤田 俊之, 秦 裕子, 近藤 裕子, 国枝 武和, 尾山 大明, 久保 健雄
  • Organizer: 第85回日本生化学会大会
  • Place of Presentation: 福岡県博多区 福岡国際会議場
• [Presentation] 熱殺蜂球形成時のニホンミツバチ脳内の神経興奮は高温情報処理を反映する (2012)
  • Author(s): 宇賀神篤、國枝武和、小野正人、吉田忠晴、久保健雄
  • Organizer: 日本昆虫学会第72回大会
  • Place of Presentation: 東京都町田市 玉川大学
• [Presentation] Ecdysteroid biosynthesis in adult workers of the European honeybee Apis mellifera L. (2011)
  • Author(s): 塩田百合香、木内信、竹内秀明、久保健雄
  • Organizer: 第34回日本分子生物学会年会
  • Place of Presentation: パシフィコ横浜
  • Year and Date: 2011-12-16
• [Presentation] Neural activity in the brains of the Japanese worker honeybees involved in a hot defensive bee ball reflects thermal stimuli processing (2011)
  • Author(s): Ugajin, A., Kiya, T., Ono, M., Yoshida, T., Kubo, T.
  • Organizer: 第34回日本分子生物学会年会
  • Place of Presentation: パシフィコ横浜
  • Year and Date: 2011-12-14
• [Presentation] Expression analysis of a long non-coding RNA, Nb-1 during oogenesis and embryogenesis and suppression of the gene expression using RNAi in the European honeybee (2011)
  • Author(s): Tadano, H., Takeuchi, H., Kubo, T.
  • Organizer: 第34回日本分子生物学会年会
  • Place of Presentation: パシフィコ横浜
  • Year and Date: 2011-12-13
• [Presentation] ミツバチの社会性行動を生み出す脳と遺伝子の仕組み (2011)
  • Author(s): 久保健雄
  • Organizer: 第2回岐阜薬科大学機能性健康食品研究講演会
  • Place of Presentation: 岐阜市ホテルグランベール岐山(招待講演)
  • Year and Date: 2011-11-26
• [Presentation] Functional characterization of hypopharyngeal gland and salivary glands of the worker honeybees by using proteomics (2011)
  • Author(s): Fujita, T., Hata, K.-H., Kondo, H., Kunieda, T., Oyama, M., Kubo, T.
  • Organizer: 3rd International Symposium Frontiers in Agriculture Proteome Kesearch Contribution of proteomics technology in agricultural sciences
  • Place of Presentation: 筑波
  • Year and Date: 2011-11-10
• [Presentation] 熱殺蜂球形成時のニホンミツバチ働き蜂の脳の神経興奮は高温情報処理を反映する (2011)
  • Author(s): 宇賀神篤、木矢剛智、國枝武和、小野正人、吉田忠晴、久保健雄
  • Organizer: 昆虫ワークショップ2011東北
  • Place of Presentation: 蔵王温泉ZAOセンタープラザ
  • Year and Date: 2011-10-12
• [Presentation] 熱殺蜂球形成時のニホンミツバチ働き蜂の脳の神経興奮は高温情報処理を反映する (2011)
  • Author(s): 宇賀神篤、木矢剛智、國枝武和、小野正人、吉田忠晴、久保健雄
  • Organizer: 日本動物学会第82回大会
  • Place of Presentation: 旭川大雪アリーナ
  • Year and Date: 2011-09-21
• [Presentation] Ecdysteroid biosynthesis in adult workers of the European honeybee Apis mellifera L. (2011)
  • Author(s): 塩田百合香、木内信、竹内秀明、久保健雄
  • Organizer: 日本動物学会 第82回 旭川大会
  • Place of Presentation: 旭川大雪アリーナ
  • Year and Date: 2011-09-21
• [Book] Ecdysteroids and Honeybee Social Bheviors. In : Hormones and Behavior (Ed: Simonsen, D.) (2013)
  • Author(s): Shiota, Y. and Kubo, T.
  • Publisher: NOVA Science Publishers(eBook)
• [Book] Ecdysteroids and Honeybee Social Bheviors. In: Hormones and Behavior (Ed: Simonsen, D.) eBook (2013)
  • Author(s): Shiota, Y. and Kubo, T.
  • Total Pages: 21
  • Publisher: eBook, NOVA Science Publishers.
• [Book] Neuroanatomical dissection of the honeybee brain based on temporal and regional gene expression patterns. In: Honeybee neurobiology and Behavior. A Tribute to Randolf Menzel (Eds: Galizia, C. G., Eisenhardt, D. and Giulfa, M.) (2012)
  • Author(s): Kubo, T
  • Publisher: Springer
• [Book] Neuroanatomical dissection of the honeybee brain based on temporal and regional gene expression patterns. In: Honeybee neurobiology and Behavior. A Tribute to Randolf Menzel (eds. Galizia CG, Eisenhardt D and Giulfa M) (2012)
  • Author(s): Kubo, T.
  • Total Pages: 18
  • Publisher: Springer
• [Book] Neuroanatomical dissection of the honeybee brain based on temporal and regional gene expression patterns In : Honeybee neurobiology and behavior-A tribute to Randolf Menzel-(Eds.Galizia, C.G., Eisenhardt, D.and Giulfa, M.) (2012)
  • Author(s): Kubo, T.
  • Total Pages: 18
  • Publisher: Springer
• [Remarks]
  • URL: http://www.biol.s.u-tokyo.ac.jp/users/saibou/lab.html
• [Remarks] 東京大学大学院理学系研究科生物科学専攻細胞生理化学研究室
  • URL: http://www.biol.s.u-tokyo.ac.jp/users/saibou/lab.html
• [Remarks] 東京大学 大学院理学系研究科 生物科学専攻 細胞生理化学研究室
  • URL: http://www.biol.s.u-tokyo.ac.jp/users/saibou/lab.html
• [Remarks]
  • URL: http://www.biol.s.u-tokyo.ac.jp/users/saibou/lab.html