| Project/Area Number |
23370051
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Structural biochemistry
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| Research Institution | Osaka University |
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Principal Investigator |
IMADA Katsumi 大阪大学, 理学(系)研究科(研究院), 教授 (40346143)
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| Co-Investigator(Renkei-kenkyūsha) |
KAWAGISHI Ikuro 法政大学, 生命科学部, 教授 (80234037)
KAWAGUCHI Tatsuya 大阪大学, 大学院・理学研究科, 助教 (10314353)
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| Project Period (FY) |
2011-11-18 – 2014-03-31
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| Project Status |
Completed (Fiscal Year 2013)
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| Budget Amount *help |
¥11,180,000 (Direct Cost: ¥8,600,000、Indirect Cost: ¥2,580,000)
Fiscal Year 2013: ¥5,590,000 (Direct Cost: ¥4,300,000、Indirect Cost: ¥1,290,000)
Fiscal Year 2012: ¥5,590,000 (Direct Cost: ¥4,300,000、Indirect Cost: ¥1,290,000)
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| Keywords | 分子機械 / 走化性センサー / 膜タンパク質 / 生体分子 |
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| Research Abstract |
In this study, we revealed the ligand recognition mechanism of Tsr, a bacterial chemo-receptor for serine, and Tcp, that for citrate, by X-ray crystallography, mutational analysis and the ITC measurements. We also elucidated the ligand binding mechanisms of Mlp24 and Mlp37, bacterial chemo-receptors with PAS-like domains, from the crystal structures of Mlp24 and Mlp37 with or without ligands and following mutational analyses.
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