Integration of membrane proteins through the endoplasmic reticulum translocon and other organelle in the eukaryotic cells
| Project/Area Number |
23370055
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Structural biochemistry
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| Research Institution | University of Hyogo |
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Principal Investigator |
SAKAGUCHI MASAO 兵庫県立大学, 生命理学研究科, 教授 (30205736)
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| Project Period (FY) |
2011-04-01 – 2014-03-31
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| Project Status |
Completed (Fiscal Year 2013)
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| Budget Amount *help |
¥20,020,000 (Direct Cost: ¥15,400,000、Indirect Cost: ¥4,620,000)
Fiscal Year 2013: ¥5,850,000 (Direct Cost: ¥4,500,000、Indirect Cost: ¥1,350,000)
Fiscal Year 2012: ¥6,760,000 (Direct Cost: ¥5,200,000、Indirect Cost: ¥1,560,000)
Fiscal Year 2011: ¥7,410,000 (Direct Cost: ¥5,700,000、Indirect Cost: ¥1,710,000)
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| Keywords | 膜タンパク質 / シグナル配列 / 膜トポロジー / 生体膜 / オルガネラ / 小胞体 / トポロジー |
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| Research Abstract |
In this project, we have addressed the protein integration through translocon on the endoplasmic reticulum in the eukaryotic cells and the targeting of membrane proteins to the other organelle. We demonstrated the follows; the membrane integration of hydrophobic sequences though translocon can be regulated by ribosomes. The movement of polypeptide chain is stalled by positively charged amino acid residues on the chain and then resumes. The forced membrane integration of marginally hydrophobic segment can be observed in the membrane integration of naturally occurring membrane protein. Cholesterol in the membrane exerts pleiotropic effects on the translocon function.
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Report
(4 results)
Research Products
(75 results)
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[Journal Article] Stop-and-move of a marginally hydrophobic segment translocating across the endoplasmic reticulum membrane2013
Author(s)
Onishi, Y., Yamagishi, M., Imai, K., Fujita, H., Kida, Y., and Sakaguchi, M
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Journal Title
J. Mol. Biol
Volume: 425
Issue: 17
Pages: 3205-3216
DOI
Related Report
Peer Reviewed
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[Journal Article] Tail-extension following the termination codon is critical for release of the nascent chain from membrane-bound ribosomes in a reticulocyte lysate cell-free system2013
Author(s)
Takahara, M., Sakaue, H., Onishi, Y., Yamagishi, M., Kida, Y., and Sakaguchi, M
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Journal Title
Biochem. Biophys. Res. Commun
Volume: 430
Issue: 2
Pages: 567-572
DOI
Related Report
Peer Reviewed
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[Presentation] ER-targeting Suppression by Short N-terminal Motif of Peroxisomal ABC Transporter, PMP702011
Author(s)
Sakaguchi, M., Iwashita, S., Yamashita, Y., Sakaue, H., Kida, Y.
Organizer
第63回日本細胞生物学会大会
Place of Presentation
北海道大学(北海道)
Related Report
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