| Project/Area Number |
23370057
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Functional biochemistry
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| Research Institution | Hokkaido University |
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Principal Investigator |
KIHARA Akio 北海道大学, 薬学研究科(研究院), 教授 (50333620)
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| Co-Investigator(Kenkyū-buntansha) |
SASSA Takayuki 北海道大学, 大学院・薬学研究院, 講師 (20342793)
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| Co-Investigator(Renkei-kenkyūsha) |
OBARA Keisuke 北海道大学, 大学院・薬学研究院, 助教 (30419858)
OHNO Yusuke 北海道大学, 大学院・薬学研究院, 助教 (50611498)
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| Project Period (FY) |
2011-04-01 – 2014-03-31
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| Project Status |
Completed (Fiscal Year 2013)
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| Budget Amount *help |
¥19,890,000 (Direct Cost: ¥15,300,000、Indirect Cost: ¥4,590,000)
Fiscal Year 2013: ¥6,890,000 (Direct Cost: ¥5,300,000、Indirect Cost: ¥1,590,000)
Fiscal Year 2012: ¥6,370,000 (Direct Cost: ¥4,900,000、Indirect Cost: ¥1,470,000)
Fiscal Year 2011: ¥6,630,000 (Direct Cost: ¥5,100,000、Indirect Cost: ¥1,530,000)
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| Keywords | 脂質 / 代謝 / 脂肪酸 / 生体膜 / 極長鎖脂肪酸 / 皮膚バリア / スフィンゴ脂質 / 代謝異常 / ノックアウトマウス / 小胞輸送 / 皮膚 / 小胞輪送 / 皮膚バリア機能 / エンドソーム / リポソーム |
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| Research Abstract |
Although cellular level of very long-chain fatty acids (VLCFAs), which have carbon chain-length greater than 20 (>C20), is less abundant than that of long-chain fatty acids (LCFAs; C11-C20), VLCFAs possess important functions that cannot be substituted for by LCFAs and related to some disorders. In this research project, we revealed the functions of VLCFAs in skin barrier formation, relationship between metabolism of VLCFAs and several disorders such as adrenoleucodystrophy, myopathy, and mental retardation, regulatory mechanism of the VLCFA synthetic enzymes by the ceramide synthases, and the roles of VLCFAs in the vesicular transport via endosomes, by physiological and cell biological analyses using knockout mice and cultured cells, by biochemical assays using purified VLCFA synthetic enzymes, and by yeast genetical approach.
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