Functions of RECQL1 and RECQL5 in the maintenance of genome stability

• Project/Area Number: 23370065
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Functional biochemistry
• Research Institution: Musashino University
• Principal Investigator: ENOMOTO Takemi 武蔵野大学, 薬学研究所, 教授 (80107383)
• Project Period (FY): 2011-04-01 – 2014-03-31
• Project Status: Completed (Fiscal Year 2013)
• Budget Amount: ¥19,890,000 (Direct Cost: ¥15,300,000、Indirect Cost: ¥4,590,000); Fiscal Year 2013: ¥6,500,000 (Direct Cost: ¥5,000,000、Indirect Cost: ¥1,500,000); Fiscal Year 2012: ¥6,500,000 (Direct Cost: ¥5,000,000、Indirect Cost: ¥1,500,000); Fiscal Year 2011: ¥6,890,000 (Direct Cost: ¥5,300,000、Indirect Cost: ¥1,590,000)
• Keywords: ゲノム安定維持機構 / 発がん抑制 / 遺伝子組換え / RecQヘリカーゼ / RECQL5 / RECQL1

Research Abstract

In this study, I mainly focused on the function of RECQL5. Analyses using RECQL5 gene knockout (KO) cells revealed that RECQL5 is involved in DNA interstrand crosslink (ICL) repair. The results obtained in this study suggest that RECQL5 functions down stream of BRCA2, after formation of Rad51-ssDNA filament probably disrupting disused filaments. Furthermore, the frequency and variation of CDDP-induced gene conversion at the immunoglobulin locus were increased in RECQL5 KOcells. These results suggest that RECQL5 plays a role in regulating the incidence and quality of ICL-induced recombination.

Research Products

• [Journal Article] Tumor suppressor RecQL5 controls recombination induced by DNA crosslinking agents (2014)
  • Author(s): Yoshifumi Hosono, Takuya Abe, Masamichi Ishiai, M. Nurul Islam, Hiroshi Arakawa, Weidong Wang, Shunichi Takeda, Yutaka Ishii, Minoru Takata, Masayuki Seki, Takemi Enomoto
  • Journal Title: Biochimica et Biophysica Acta-Molecular Cell Research Volume: 1843 Issue: 5 Pages: 1002-1012
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  • Peer Reviewed / Open Access
• [Journal Article] Tipin Function in the Protection against Topoisomerase I Inhibitor (2014)
  • Author(s): Yoshifumi Hosono, Takuya Abe, Masato Higuchi, Kosa Kajii, Shuichi Sakuraba, Shusuke Tada, Takemi Enomoto, Masayuki Seki
  • Journal Title: The Journal of Biological Chemistry Volume: 289 Issue: 16 Pages: 11374-11384
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• [Journal Article] Roles of common subunits within distinct multisubunit complexes (2014)
  • Author(s): Y. Nakabayashi, S. Kawashima, T. Enomoto, M. Seki, M. Horikoshi
  • Journal Title: Proc. Natl. Acad. Sci. USA Volume: 111 Issue: 2 Pages: 699-704
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  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Protective roles of ascorbic acid in oxidative stress induced by depletion of superoxide dismutase in vertebrate cells (2013)
  • Author(s): Tamari, Y., Nawata, H., Inoue, E., Yoshimura, A., Yoshii, H., Kashino, G., Seki, M., Enomoto, T., Watanabe, M., and Tano, K
  • Journal Title: Free Rad. Res Volume: 47 Issue: 1 Pages: 1-7
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  • NAID: 120005398557
  • Peer Reviewed
• [Journal Article] Rmi1 functions in S phase-mediated cohesion establishment via a pathway involving the Ctf18-RFC complex and Mrc1 (2012)
  • Author(s): Lai, M.S., Seki, M., Tada, S., and Enomoto, T
  • Journal Title: Biochem. Biophys. Res. Commun Volume: 427 Pages: 682-686
• [Journal Article] NIH3T3 cells overexpressing CD98 heavy chain resist early G1 arrest and apoptosis induced by serum starvation (2012)
  • Author(s): Hara, K., Ueda, S., Ohno, Y., Tanaka, T., Yagi, H., Okazaki, S., Kawahara, R., Masayuki, T., Enomoto, T., Hashimoto, Y., Masuko, K., and Masuko, T
  • Journal Title: Cancer Sci Volume: 103
• [Journal Article] An FD-LC-MS/MS proteomic strategy for revealing cellular protein networks : A conditional superoxide dismutase 1 knockout cells (2012)
  • Author(s): Ichibangase, T., Sugawara, Y., Yamabe, A., Koshiyama, A., Yoshimura, A., Enomoto, T., and Imai, K
  • Journal Title: PLOS ONE Volume: 7 Issue: 9 Pages: e45483-e45483
  • DOI: 10.1371/journal.pone.0045483
  • Peer Reviewed
• [Journal Article] WRNIP1 accumulates at laser light irradiated sites rapidly via its ubiquitin-binding zinc finger domain and independently from its ATPase domain. (2012)
  • Author(s): Nomura, H., Yoshimura, A., Edo, T., Kanno, S.I., Tada, S., Seki, M., Yasui, A., and Enomoto, T.
  • Journal Title: Biochem. Biophys. Res. Commun. Volume: 417 Issue: 4 Pages: 1145-1150
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  • Peer Reviewed
• [Journal Article] Nucleosome surface containing nucleosomal DNA entry/exit site regulates H3-K36me3 via DNA entry/exit site regulates H3-K36me3 via (2012)
  • Author(s): H.Endo, Y.Nakabayashi, S.Kawashima, T.Enomoto, M.Seki & M.Horikoshi
  • Journal Title: Genes Cells Volume: 17 Issue: 1 Pages: 65-81
  • DOI: 10.1111/j.1365-2443.2011.01573.x
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• [Journal Article] Embryonic lethality in mice lacking mismatch-specific thymine DNA glycosylase is partially prevented by DOPS a precursor of noradrenarine (2012)
  • Author(s): Saito, Y., Ono, T., Takeda, N., Nohmi, T., Seki, M., Enomoto, T., Noda, T., and Uehara Y
  • Journal Title: Tohoku J. Exp. Med Volume: 226 Pages: 75-83
• [Journal Article] NIH3T3 cells overexpressing CD98 heavy chain resist early G1 arrest andapoptosis induced by serum starvation. (2012)
  • Author(s): Hara K, Ueda S, Ohno Y, Tanaka T, Yagi H, Okazaki S, Kawahara R, Masayuki T, Enomoto T, Hashimoto Y, Masuko K, Masuko T.
  • Journal Title: Cancer Sci Volume: 103 Issue: 8 Pages: 1460-1466
  • DOI: 10.1111/j.1349-7006.2012.02304.x
  • Peer Reviewed
• [Journal Article] Rmi1 functions in S phase-mediated cohesion establishment via a pathway involving the Ctf18-RFC complex and Mrc1. (2012)
  • Author(s): Lai, M.S., *Seki, M., Tada, S., and Enomoto T.
  • Journal Title: Biochem. Biophys. Res. Commun. Volume: 417 Issue: 3 Pages: 1145-1150
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  • Peer Reviewed
• [Journal Article] Nucleosome surface containing nucleosomal DNA entry/exit site regulates H3-K36me3 via association with RNA polymerase II and Set2 (2012)
  • Author(s): Endo, H.
  • Journal Title: Genes Cells Volume: VOL.17 Pages: 65-81
  • Peer Reviewed
• [Journal Article] WRNIP1 accumulates at laser light irradiated sites rapidly via its ubiquitin-binding zinc finger domain and independently from its ATPase domain (2012)
  • Author(s): Nomura, H.
  • Journal Title: Biochem.Biophys.Res.Commun. Volume: VOL.417 Pages: 1145-1150
  • Peer Reviewed
• [Journal Article] Functional relationship between Claspin and Rad17 (2011)
  • Author(s): Yoshimura, A., Akita, M., Hosono, Y., Abe, T., Kobayashi, M., Yamamoto, K.I., Tada, S., Seki, M., Enomoto, T.
  • Journal Title: Biochem.Biophys.Res.Commun. Volume: 414 Issue: 2 Pages: 298-303
  • DOI: 10.1016/j.bbrc.2011.09.037
  • Peer Reviewed
• [Journal Article] The roles of histone chaperone CIA/Asf1 in nascent DNA elongation during nucleosome replication. (2011)
  • Author(s): K.Ishikawa, T.Ohsumi, S.Tada, R.Natsume, L.R.Kundu, N.Nozaki, T.Senda, T.Enomoto, M.Horikoshi & M.Seki
  • Journal Title: Genes Cells Volume: 16 Issue: 10 Pages: 1050-1062
  • DOI: 10.1111/j.1365-2443.2011.01549.x
  • Peer Reviewed
• [Journal Article] The histone chaperone FACT maintains replication fork rates (2011)
  • Author(s): T.Abe, K.Sugimura, Y.Hosono, Y.Takami, M.Akita, A.Yoshimura, S.Tada, T.Nakayama, H.Murofushi, K.Okumura, S.Takeda, M.Horikoshi, M.Seki & T.Enomoto
  • Journal Title: J.Biol.Chem. Volume: 286 Issue: 35 Pages: 30504-30512
  • DOI: 10.1074/jbc.m111.264721
  • Peer Reviewed
• [Journal Article] Global analysis of core histones reveals nucleosomal surfaces required for chromosomebi-orientation (2011)
  • Author(s): S.Kawashima, Y.Nakabayashi, K.Matsubara, N.Sano, T.Enomoto, K.Tanaka, M.Seki & M.Horikoshi
  • Journal Title: EMBO J. Volume: 30 Issue: 16 Pages: 3353-3367
  • DOI: 10.1038/emboj.2011.241
  • Peer Reviewed
• [Journal Article] The role of SNM1 family nucleases in etoposide-induced apoptosis (2011)
  • Author(s): Hosono, Y., Abe, T., Ishiai, M., Takata, M., Enomoto, T., Seki, M.
  • Journal Title: Biochem.Biophys.Res.Commun. Volume: 410 Issue: 3 Pages: 568-573
  • DOI: 10.1016/j.bbrc.2011.06.027
  • Peer Reviewed
• [Journal Article] Werner interacting protein 1 promotes binding ofWerner protein to template-primer DNA (2011)
  • Author(s): Kanamori, M., Seki, M., Yoshimura, A., Tsurimoto, T., Tada, S., and Enomoto, T.
  • Journal Title: Biol. Pharm. Bull Volume: 34 Pages: 1314-1318
• [Journal Article] Biphasic chromatin binding of histone chaperone FACT during eukaryotic chromatin DNA replication (2011)
  • Author(s): L.R.Kundu, M.Seki, H.Murofushi,A.Furukohri, S.Waga, A.J.Score, J.J.Blow, M.Horikoshi, T.Enomoto & S.Tada
  • Journal Title: Biochim.Biophys.Acta Volume: 1813 Issue: 6 Pages: 1129-36
  • DOI: 10.1016/j.bbamcr.2011.01.002
  • Peer Reviewed
• [Journal Article] The N-terminal region of RECQL4 lacking the helicase domain is both essential and sufficient for the viability of vertebrate cells (2011)
  • Author(s): Abe, T., Yoshimura, A., Hosono, Y., Tada, S., Seki, M., Enomoto. T
  • Journal Title: Biochim.Biophys.Acta Volume: 1813 Issue: 3 Pages: 473-479
  • DOI: 10.1016/j.bbamcr.2011.01.001
  • Peer Reviewed
• [Journal Article] Biphasic chromatin binding of FACT during DNA replication (2011)
  • Author(s): Kundu, L.R.
  • Journal Title: Biochim.Biophys.Acta Volume: VOL.1813 Pages: 1129-1136
  • Peer Reviewed
• [Journal Article] Werner Interacting Protein 1 Promotes Binding of Werner Protein to Template-Primer DNA (2011)
  • Author(s): Kanamori M.
  • Journal Title: Biol.Pharm.Bull. Volume: VOL.34 Pages: 1314-1318
  • Peer Reviewed
• [Journal Article] The role of SNM1 family nucleases in etoposide-induced apoptosis (2011)
  • Author(s): Hosono, Y.
  • Journal Title: Biochem.Biophys.Res.Commun. Volume: VOL.410 Pages: 568-573
  • Peer Reviewed
• [Journal Article] Clobal analysis of core histones reveals nucleosomal surfaces required for chromosome bi-orientation (2011)
  • Author(s): Kawashima, S.
  • Journal Title: EMBO J. Volume: VOL.30 Pages: 3353-3367
  • Peer Reviewed
• [Journal Article] The histone chaperone FACT maintains normal replication rates (2011)
  • Author(s): Abe, T.
  • Journal Title: J.Biol.Chem. Volume: VOL.286 Pages: 30504-30512
  • Peer Reviewed
• [Journal Article] Roles of histone chaperone CIA/Asf1 in nascent DNA elongation during nucleosome replication (2011)
  • Author(s): Ishikawa, K.
  • Journal Title: Genes Cells Volume: VOL.16 Pages: 1050-1062
  • Peer Reviewed
• [Journal Article] Functional relationship between Claspin and Rad17 (2011)
  • Author(s): Yoshimura, A.
  • Journal Title: Biochem.Biophys.Res.Commun. Volume: VOL.414 Pages: 298-303
  • Peer Reviewed
• [Presentation] WRNIP1欠損によるPolη破壊株のUV感受性抑制機構の解析 (2014)
  • Author(s): 吉村明
  • Organizer: 日本薬学会第134年会
  • Place of Presentation: 熊本市
• [Presentation] カンプトテシン存在下におけるDNA複製フォーク構成因子Tipinの機能解析 (2014)
  • Author(s): 梶井孔左
  • Organizer: 日本薬学会第134年会
  • Place of Presentation: 熊本市
• [Presentation] WRNIP1 regulates DNA polymerase eta in DNA damage tolerance (2013)
  • Author(s): 吉村明, 薗田覚, 関政幸, 榎本武美
  • Organizer: 第86回日本生化学大会
  • Place of Presentation: パシフィコ横浜(横浜市)
  • Year and Date: 2013-09-13
• [Presentation] WRNIP1 regulates DNA polymerase eta in DNA damage tolerance (2013)
  • Author(s): 吉村明
  • Organizer: 第86回日本生化学大会
  • Place of Presentation: 横浜市
• [Presentation] 遺伝子破壊細胞を用いたDNA複製フォーク構成因子Tipinのカンプトテシン毒性防御機構における機能の解析 (2013)
  • Author(s): 細野嘉史
  • Organizer: 第22回DNA複製・組換え・修復ワークショップ
  • Place of Presentation: 仙台市
• [Presentation] Role of DNA replication fork component Tipin upon the treatment of Top1 inhibitor camptothecin (2013)
  • Author(s): 梶井孔左
  • Organizer: 第36回日本分子生物学会年会
  • Place of Presentation: 神戸市
• [Presentation] Pol η破壊株のUV感受性にWRNIP1の欠損が及ぼす影響の解析 (2013)
  • Author(s): 吉村明
  • Organizer: 第36回日本分子生物学会年会
  • Place of Presentation: 神戸市
• [Presentation] RecQヘリカーゼのDNAクロスリンク修復における役割 (2012)
  • Author(s): 細野嘉史, 関政幸, 阿部拓也, 石合正道, 武田俊一, 石井裕, 高田穣, 榎本武美
  • Organizer: 第35回日本分子生物学会年会
  • Place of Presentation: 福岡国際会議場(福岡市)
  • Year and Date: 2012-12-11
• [Presentation] Function of RecQL5 helicase in interstrand crosslink repair (2012)
  • Author(s): Yoshifumi Hosono, Masayuki Seki, Takuya Abe, Masamichi Ishiai, Shunichi Takeda, Ishii Yutaka, Minoru Takata, Takemi Enomoto
  • Organizer: The 8th 3R Symposium
  • Place of Presentation: 淡路夢舞台(淡路市)
  • Year and Date: 2012-11-26
• [Presentation] Function of RecQL5 helicase in interstrand crosslink repair (2012)
  • Author(s): 細野 嘉史
  • Organizer: The 8th 3R Symposium
  • Place of Presentation: 淡路夢舞台（淡路市）
• [Presentation] RecQL5ヘリカーゼのDNAクロスリンク修復における役割 (2012)
  • Author(s): 細野 嘉史
  • Organizer: 第35回日本分子生物学会年会
  • Place of Presentation: 福岡国際会議場（福岡市）
• [Presentation] 共通サブユニットとしてのヒストンH2B機能解析から新たに明らかとなった異 なるヌクレオソーム間での機能残基の違い (2012)
  • Author(s): 中林 悠
  • Organizer: 第35回日本分子生物学会年会
  • Place of Presentation: 福岡国際会議場（福岡市）
• [Presentation] ヒストンH2AとそのバリアントHtz1の分子表面の解析 (2012)
  • Author(s): 上野 岳
  • Organizer: 第35回日本分子生物学会年会
  • Place of Presentation: 福岡国際会議場（福岡市）
• [Presentation] WRNIP1とPol etaのDNA損傷における機能的連携の解析 (2012)
  • Author(s): 吉村 明
  • Organizer: 第35回日本分子生物学会年会
  • Place of Presentation: 福岡国際会議場（福岡市）
• [Presentation] Analysis of the functional relationship between WRNIP1 and DNA polymerase eta in the cell (2011)
  • Author(s): 吉村明, 関政幸, 榎本武美
  • Organizer: 第34回日本分子生物学会年会
  • Place of Presentation: パシフィコ横浜(横浜市)
  • Year and Date: 2011-12-15
• [Presentation] Analysis of the functional relationship between WRNIP1 and DNA polymerase etha in the cell (2011)
  • Author(s): 吉村明
  • Organizer: 第34回日本分子生物学会年会
  • Place of Presentation: パシフィコ横浜(横浜市)
  • Year and Date: 2011-12-15
• [Presentation] Global analysis of functional relationships between H2A and its variant Htz1 (2011)
  • Author(s): 上野岳
  • Organizer: 第34回日本分子生物学会年会
  • Place of Presentation: パシフィコ横浜(横浜市)
  • Year and Date: 2011-12-15
• [Presentation] DNAヘリカーゼRecQL5の相同組換え機構における機能の解析 (2011)
  • Author(s): 細野嘉史, 阿部拓也, 関政幸, 榎本武美
  • Organizer: DNAヘリカーゼRecQL5の相同組換え機構における機能の解析
  • Place of Presentation: サンピア福岡(福津市)
  • Year and Date: 2011-10-25
• [Presentation] DNAヘリカーゼRecQL5の相同組換え機構における機能の解析 (2011)
  • Author(s): 細野嘉史
  • Organizer: 第21回DNA複製・組換え・ゲノム安定性制御ワークショップ
  • Place of Presentation: サンピア福岡(福岡県)
  • Year and Date: 2011-10-25