Single-molecule structure-function analysis of the pH-dependent gating of the KcsA potassium channel
| Project/Area Number |
23370067
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Biophysics
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| Research Institution | University of Fukui |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
SHIMIZU Hirofumi 福井大学, 医学部, 講師 (50324158)
IWAMOTO Masayuki 福井大学, 医学部, 助教 (40452122)
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| Project Period (FY) |
2011-04-01 – 2014-03-31
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| Project Status |
Completed (Fiscal Year 2013)
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| Budget Amount *help |
¥20,670,000 (Direct Cost: ¥15,900,000、Indirect Cost: ¥4,770,000)
Fiscal Year 2013: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
Fiscal Year 2012: ¥3,640,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥840,000)
Fiscal Year 2011: ¥14,820,000 (Direct Cost: ¥11,400,000、Indirect Cost: ¥3,420,000)
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| Keywords | イオンチャネル / ゲーティング / 構造変化 / 膜内構造 / 集合・離散 / 一分子測定 / 立体構造 / 単一チャネル電流 / ｐHジャンプ / 一分子計測 / 濃度ジャンプ |
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| Research Abstract |
The pH-dependent gating of the KcsA potassium channel was examined by enhancing the spacial and temporal resolutions of the diffracted X-ray tracking method. Unlike previous steady-state measurements, the solution pH was jumped by using the caged compound, and transient behavior of the gating conformational change was recorded with the high-speed image sampling (5000 frame/sec). We found that the clockwise twisting was initiated upon acidic pH change. By using the atomic force microscopy (AFM), we revealed the substantial shortening of the longitudinal length of the KcsA channel in the membrane embedded condition. These results indicate that channel shows a spring-like motion, involving simultaneous twisting and shortening, upon opening of the channel.
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Report
(4 results)
Research Products
(98 results)
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[Journal Article] ATR-FTIR Spectroscopy Revealed the Different Vibrational Modes of the Selectivity Filter Interacting with K+ and Na+ in the Open and Collapsed Conformations of the KcsA Potassium Channel2012
Author(s)
Furutani, Y., Shimizu, H., Asai, Y., Fukuda, T., Oiki, S. and Kandori, H
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Journal Title
J. Phys. Chem. Lett
Volume: 3
Issue: 24
Pages: 3806-3810
DOI
Related Report
Peer Reviewed
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