| Project/Area Number |
23370070
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Biophysics
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| Research Institution | Kyoto University |
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Principal Investigator |
IMAMOTO Yasushi 京都大学, 理学(系)研究科(研究院), 准教授 (80263200)
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| Co-Investigator(Kenkyū-buntansha) |
SHICHIDA Yoshinori 京都大学, 大学院・理学研究科, 教授 (60127090)
YAMASHITA Takahiro 京都大学, 大学院・理学研究科, 助教 (50378535)
SAKO Yasushi 理化学研究所, 主任研究員 (20215700)
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| Co-Investigator(Renkei-kenkyūsha) |
OKA Toshihiko 静岡大学, 大学院・理学研究科, 講師 (60344389)
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| Project Period (FY) |
2011-04-01 – 2014-03-31
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| Project Status |
Completed (Fiscal Year 2013)
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| Budget Amount *help |
¥20,410,000 (Direct Cost: ¥15,700,000、Indirect Cost: ¥4,710,000)
Fiscal Year 2013: ¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000)
Fiscal Year 2012: ¥5,330,000 (Direct Cost: ¥4,100,000、Indirect Cost: ¥1,230,000)
Fiscal Year 2011: ¥10,660,000 (Direct Cost: ¥8,200,000、Indirect Cost: ¥2,460,000)
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| Keywords | 平衡 / 一分子計測 / X線高角散乱 / 赤外分光 / 構造変化 / アゴニスト / 構成的活性変異 / ロドプシン / Ｘ線散乱 / X線散乱 |
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| Research Abstract |
The activation process of G protein-coupled receptors was investigated based on the structural dynamics using rhodopsin. The effect of the ligand on the structure and equilibrium of the active state was analyzed by high-angle solution X-ray scattering, single-molecule fluorometry, and Fourier-transform infrared spectroscopy. Our results demonstrated that G protein-coupled receptors are in the equilibrium between active and inactive conformations, and the inverse-agonist and agonist bias the equilibrium toward inactive and active conformations, respectively, to regulate the activity.
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