| Budget Amount *help |
¥18,720,000 (Direct Cost: ¥14,400,000、Indirect Cost: ¥4,320,000)
Fiscal Year 2013: ¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000)
Fiscal Year 2012: ¥6,890,000 (Direct Cost: ¥5,300,000、Indirect Cost: ¥1,590,000)
Fiscal Year 2011: ¥7,800,000 (Direct Cost: ¥6,000,000、Indirect Cost: ¥1,800,000)
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| Outline of Final Research Achievements |
We have developed a system that can observe the beating of myotubes differentiated to skeletal muscle cells. We have also established a system for live imaging while monitoring differentiation markers by qRT-PCR to identify the state of actin fibers. Additionally, we have combined suitable vitrification and dual-axial observation in order to obtain a clearer image of the cell. Using this EM system, we could observe relatively thick specimens and distinguish actin fibers from microtubules and intermediate filaments more clearly. In addition to the visualization of the superfine structure without staining, we successfully observed in the same field of the cell under liquid N2 using light microscopy to identify target molecules.
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