X-ray structural analysis of the 380-kDa motor domain of cytoplasmic dynein
| Project/Area Number |
23370073
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Biophysics
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| Research Institution | Hosei University (2013)
Osaka University (2011-2012) |
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Principal Investigator |
KON Takahide 法政大学, 生命科学部, 教授 (30332620)
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| Co-Investigator(Renkei-kenkyūsha) |
KURISU Genji 大阪大学, 蛋白質研究所, 教授 (90294131)
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| Project Period (FY) |
2011-04-01 – 2014-03-31
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| Project Status |
Completed (Fiscal Year 2013)
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| Budget Amount *help |
¥19,760,000 (Direct Cost: ¥15,200,000、Indirect Cost: ¥4,560,000)
Fiscal Year 2013: ¥7,150,000 (Direct Cost: ¥5,500,000、Indirect Cost: ¥1,650,000)
Fiscal Year 2012: ¥8,320,000 (Direct Cost: ¥6,400,000、Indirect Cost: ¥1,920,000)
Fiscal Year 2011: ¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000)
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| Keywords | 構造生物学 / モータータンパク質 / X線結晶構造解析 / ナノマシン / 酵素反応 / 分子モーター / 生物物理 / ナノバイオ / 蛋白質 |
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| Research Abstract |
Dyneins are huge microtubule-based motor complexes that power a wide variety of biological processes within eukaryotic cells, including cell division, cell migration, and the intracellular trafficking. However, the mechanism by which dynein generates force and movement still remains unclear. Here we have determined the crystal structure of the 380-kDa motor domain of Dictyostelium cytoplasmic dynein at 2.8-A resolution. This long sought crystal structure, together with the results of our EM and functional analyses, provides a framework for understanding the mechanism underlying dynein-based motility.
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Report
(4 results)
Research Products
(39 results)
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[Journal Article] The 2.8 Å crystal structure of the dynein motor domain2012
Author(s)
Kon, T., Oyama, T., Shimo-Kon, R., Imamula, K., Shima, T., Sutoh, K., Kurisu, G
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Journal Title
Nature
Volume: 484
Pages: 345-350
Related Report
Peer Reviewed
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[Journal Article] ATP-Driven Remodeling of the Linker Domain in the Dynein Motor2012
Author(s)
Roberts, A.J., Malkova, B., Walker, M.L., Sakakibara, H., Numata, N., Kon, T., Ohkura, R., Edwards, T.A., Knight, P.J., Sutoh, K., Oiwa, K., Burgess, S.A
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Journal Title
Structure
Volume: 20
Pages: 1670-1680
Related Report
Peer Reviewed
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