X-ray structural analysis of the 380-kDa motor domain of cytoplasmic dynein

• Project/Area Number: 23370073
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Biophysics
• Research Institution: Hosei University (2013); Osaka University (2011-2012)
• Principal Investigator: KON Takahide 法政大学, 生命科学部, 教授 (30332620)
• Co-Investigator(Renkei-kenkyūsha): KURISU Genji 大阪大学, 蛋白質研究所, 教授 (90294131)
• Project Period (FY): 2011-04-01 – 2014-03-31
• Project Status: Completed (Fiscal Year 2013)
• Budget Amount: ¥19,760,000 (Direct Cost: ¥15,200,000、Indirect Cost: ¥4,560,000); Fiscal Year 2013: ¥7,150,000 (Direct Cost: ¥5,500,000、Indirect Cost: ¥1,650,000); Fiscal Year 2012: ¥8,320,000 (Direct Cost: ¥6,400,000、Indirect Cost: ¥1,920,000); Fiscal Year 2011: ¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000)
• Keywords: 構造生物学 / モータータンパク質 / X線結晶構造解析 / ナノマシン / 酵素反応 / 分子モーター / 生物物理 / ナノバイオ / 蛋白質

Research Abstract

Dyneins are huge microtubule-based motor complexes that power a wide variety of biological processes within eukaryotic cells, including cell division, cell migration, and the intracellular trafficking. However, the mechanism by which dynein generates force and movement still remains unclear. Here we have determined the crystal structure of the 380-kDa motor domain of Dictyostelium cytoplasmic dynein at 2.8-A resolution. This long sought crystal structure, together with the results of our EM and functional analyses, provides a framework for understanding the mechanism underlying dynein-based motility.

Research Products

• [Journal Article] Functions and mechanics of dynein motor proteins (2013)
  • Author(s): Roberts, A.J., Kon, T., Knight, P.J., Sutoh, K., Burgess, S.A
  • Journal Title: Nature Rev. Mol. Cell Biol Volume: 14 Pages: 713-726
  • Peer Reviewed
• [Journal Article] 細胞中心方向輸送エンジン : 細胞質ダイニンの構造と運動メカニズム (2013)
  • Author(s): 昆隆英, 栗栖源嗣
  • Journal Title: 生化学 Volume: 85(4) Pages: 272-275
• [Journal Article] Functions and mechanics of dynein motor proteins (2013)
  • Author(s): Roberts, A.J., Kon, T., Knight, P.J., Sutoh, K., Burgess, S.A.
  • Journal Title: Nature Rev. Mol. Cell Biol. Volume: 14 Issue: 11 Pages: 713-726
  • DOI: 10.1038/nrm3667
  • Peer Reviewed
• [Journal Article] 細胞中心方向輸送エンジン：細胞質ダイニンの構造と運動メカニズム (2013)
  • Author(s): 昆 隆英, 栗栖源嗣
  • Journal Title: 生化学 Volume: 85 Pages: 272-275
• [Journal Article] The 2.8 Å crystal structure of the dynein motor domain (2012)
  • Author(s): Kon, T., Oyama, T., Shimo-Kon, R., Imamula, K., Shima, T., Sutoh, K., Kurisu, G
  • Journal Title: Nature Volume: 484 Pages: 345-350
  • Peer Reviewed
• [Journal Article] ATP-Driven Remodeling of the Linker Domain in the Dynein Motor (2012)
  • Author(s): Roberts, A.J., Malkova, B., Walker, M.L., Sakakibara, H., Numata, N., Kon, T., Ohkura, R., Edwards, T.A., Knight, P.J., Sutoh, K., Oiwa, K., Burgess, S.A
  • Journal Title: Structure Volume: 20 Pages: 1670-1680
  • Peer Reviewed
• [Journal Article] The 2.8 Å crystal structure of the dynein motor domain (2012)
  • Author(s): Kon T, Oyama T, Shimo-Kon R, Imamula K, Shima T, Sutoh K, Kurisu G
  • Journal Title: Nature Volume: (印刷中) Issue: 7394 Pages: 345-350
  • DOI: 10.1038/nature10955
  • Peer Reviewed
• [Journal Article] X-ray structure of a functional full-length dynein motor domain (2011)
  • Author(s): Kon, T., Sutoh, K., Kurisu, G
  • Journal Title: Nature Struct Mol Biol Volume: 18(6) Pages: 638-642
  • Peer Reviewed
• [Journal Article] 細胞質ダイニンの構造と機能のモジュール性 (2011)
  • Author(s): 島知弘, 須藤和夫, 昆隆英
  • Journal Title: 生物物理 Volume: 51(3) Pages: 118-123
  • NAID: 10029139922
• [Journal Article] X-ray structure of a functional full-length dynein motor domain (2011)
  • Author(s): Kon, T., Sutoh, K., Kurisu, G.
  • Journal Title: Nature Struct.Mol.Biol Volume: 18 Issue: 6 Pages: 638-642
  • DOI: 10.1038/nsmb.2074
  • Peer Reviewed
• [Presentation] Structural and functional analysis of dynein motors (2013)
  • Author(s): Takahide Kon
  • Organizer: International work shop Dynein2013
  • Place of Presentation: KOBE FASHION MUSEUM, Hyogo, Japan
  • Year and Date: 2013-11-01
  • Invited
• [Presentation] Structural and functional analysis of dynein motors (2013)
  • Author(s): Takahide Kon
  • Organizer: Alpbach Workshop on Molecular Motors 2013
  • Place of Presentation: Congress Centrum Alpbach, Tyrol, Austria
  • Invited
• [Presentation] Structural basis of dynein motility (2012)
  • Author(s): Takahide Kon
  • Organizer: 第50回日本生物物理学年会
  • Place of Presentation: 名古屋大学
  • Year and Date: 2012-09-22
• [Presentation] 結晶化後処理等による分解能向上例-12Åから2.8Åへ, 巨大分子モーターの場合 (2012)
  • Author(s): 昆隆英
  • Organizer: 蛋白研セミナー「高難度結晶の分解能向上に秘策はあるか?」
  • Place of Presentation: 大阪大学
  • Year and Date: 2012-09-21
• [Presentation] The 2.8-Å crystal structure of the dynein motor domain (2012)
  • Author(s): Takahide Kon, Takuji Oyama, Rieko Shimo-Kon, Kazuo Sutoh, Genji Kurisu
  • Organizer: 第12回日本蛋白質科学会年会
  • Place of Presentation: 名古屋国際会議場
  • Year and Date: 2012-06-20
• [Presentation] 結晶構造から見えてきたダイニンの力発生機構 (2012)
  • Author(s): 昆隆英
  • Organizer: 第2回分子モーター討論会
  • Place of Presentation: 東京大学本郷キャンパス
  • Year and Date: 2012-06-07
• [Presentation] Structural and functional analysis of dynein motors (2012)
  • Author(s): Takahide Kon
  • Organizer: 6th International Conference on Structural Analysis of Supramo lecular Assemblies by Hybrid Methods
  • Place of Presentation: Granlibakken Conference Center Lodge, Lake Tahoe, CA, USA(招待講演)
  • Year and Date: 2012-03-16
• [Presentation] ダイニンモータードメインの2.8Å結晶構造 (2012)
  • Author(s): 昆隆英, 大山拓次, 下-昆理恵子, 須藤和夫, 栗栖源嗣
  • Organizer: 生体運動研究合同班会議
  • Place of Presentation: 筑波大学
  • Year and Date: 2012-01-07
• [Presentation] ダイニンモータードメインの2.8Å結晶構造 (2012)
  • Author(s): 昆隆英
  • Organizer: 生体運動研究合同班会議
  • Place of Presentation: 筑波大学
  • Year and Date: 2012-01-07
• [Presentation] Structural and functional analysis of dynein motors (2012)
  • Author(s): Takahide Kon
  • Organizer: UK-Japan Symposium for Mechanochemical Cell Biology
  • Place of Presentation: Scarman House, University of Warwick, Coventry, UK
  • Invited
• [Presentation] Structural and functional analysis of dynein motors (2012)
  • Author(s): Takahide Kon
  • Organizer: 6th International Conference on Structural Analysis of Supramolecular Assemblies by Hybrid Methods
  • Place of Presentation: Granlibakken Conference Center Lodge, Lake Tahoe, CA, USA
  • Invited
• [Presentation] The 2.8-Å crystal structure of the dynein motor domain (2011)
  • Author(s): Takahide Kon, Takuji Oyama, Rieko Shimo-Kon, Kazuo Sutoh, Genji Kurisu
  • Organizer: The American Society for Cell Biology 51th Annual Meeting
  • Place of Presentation: Colorado Convention Center, Denver, CO, USA
  • Year and Date: 2011-12-05
• [Presentation] The 2.8-A crystal structure of the dynein motor domain (2011)
  • Author(s): Takahide Kon
  • Organizer: The American Society for Cell Biology 51th Annual Meeting
  • Place of Presentation: Colorado Convention Center, Denver, CO.USA
  • Year and Date: 2011-12-05
• [Presentation] ダイニンモータードメインの結晶構造 (2011)
  • Author(s): 昆隆英
  • Organizer: 第6回鞭毛・ダイニン機能研究会
  • Place of Presentation: 東京大学理学部2号館
  • Year and Date: 2011-10-01
• [Presentation] ダイニンモータードメインの結晶構造 (2011)
  • Author(s): 昆隆英
  • Organizer: 第6回鞭毛・ダイニン機能研究会
  • Place of Presentation: 東京大学
  • Year and Date: 2011-10-01
• [Presentation] Crystal Structure of the Dynein Motor Domain at 5 A Resolution (2011)
  • Author(s): Takahide Kon
  • Organizer: 第49回日本生物物理学年会
  • Place of Presentation: 兵庫県立大書写キャンパス
  • Year and Date: 2011-09-16
• [Presentation] Crystal Structure of the Dynein Motor Domain at 5 A Resolution (2011)
  • Author(s): Takahide Kon
  • Organizer: Gordon Research Conference-Muscle & Motors
  • Place of Presentation: Colby-Sawyer College, New London, NH, USA(招待講演)
  • Year and Date: 2011-06-11
• [Presentation] Crystal Structure of the Dynein Motor Domain at 5 A Resolution (2011)
  • Author(s): Takahide Kon
  • Organizer: Gordon Research Conference-Muscle & Motors
  • Place of Presentation: Colby-Sawyer College, New London, NH, USA
  • Invited
• [Presentation] Structural and functional analysis of dynein motors
  • Author(s): Takahide Kon
  • Organizer: International workshop Dynein2013
  • Place of Presentation: KOBE FASHION MUSEUM
  • Invited
• [Presentation] Structural and functional analysis of dynein motors
  • Author(s): Takahide Kon
  • Organizer: Alpbach Workshop on Molecular Motors 2013
  • Place of Presentation: Congress Centrum Alpbach, A-6236 Alpbach 246 Tyrol, Austria
  • Invited
• [Presentation] 結晶化後処理等による分解能向上例－12Åから2.8Åへ，巨大分子モーターの場合
  • Author(s): 昆 隆英
  • Organizer: 蛋白研セミナー「高難度結晶の分解能向上に秘策はあるか？」
  • Place of Presentation: 大阪大学
• [Presentation] Structural and functional analysis of dynein motors
  • Author(s): Takahide Kon
  • Organizer: UK-Japan Symposium for Mechanochemical Cell Biology
  • Place of Presentation: Scarman House, University of Warwick, Gibbet Hill Road, Coventry, CV4 7AL, UK.
  • Invited
• [Presentation] The 2.8Å; crystal structure of the dynein motor domain
  • Author(s): 昆 隆英
  • Organizer: 第12回日本蛋白質科学会年会
  • Place of Presentation: 名古屋国際会議場
• [Presentation] 結晶構造から見えてきたダイニンの力発生機構
  • Author(s): 昆 隆英
  • Organizer: 第2回分子モーター討論会
  • Place of Presentation: 東京大学
  • Invited
• [Book] Dynein | Cytoplasmic Dynein : its ATPase Cycle and ATPase-dependent Structural Changes. Comprehensive Biophysics, 1st Edition (Editor in Chief: Edward Egelman) (2012)
  • Author(s): Kon, T., Shima, T., Sutoh, K
  • Publisher: Elsevier
• [Remarks] Crystal structure of the dynein motor domain
  • URL: http://www.protein.osaka-u.ac.jp/jpn/achievement/papers/the-28-a-crystal-structure-of.php
• [Remarks] 生体内の運び屋分子『ダイニン』の原子構造を解明
  • URL: http://www.spring8.or.jp/ja/news_publications/press_release/2012/120308
• [Remarks]
  • URL: http://www.protein.osaka-u.ac.jp/jpn/achievement/papers/post-3.php
• [Remarks]
  • URL: http://www.spring8.or.jp/ja/news_publications/press_release/2011/110523