Extraction and application of structural and functional elements of proteins.
| Project/Area Number |
23370074
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Biophysics
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| Research Institution | Nara Institute of Science and Technology |
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Principal Investigator |
KAMIKUBO Hironari 奈良先端科学技術大学院大学, 物質創成科学研究科, 准教授 (20311128)
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| Co-Investigator(Kenkyū-buntansha) |
KATAOKA Mikio 奈良先端科学技術大学院大学, 物質創成科学研究科, 教授 (30150254)
YAMAZAKI Yoichi 奈良先端科学技術大学院大学, 物質創成科学研究科, 助教 (40332770)
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| Project Period (FY) |
2011-11-18 – 2014-03-31
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| Project Status |
Completed (Fiscal Year 2013)
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| Budget Amount *help |
¥9,360,000 (Direct Cost: ¥7,200,000、Indirect Cost: ¥2,160,000)
Fiscal Year 2013: ¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000)
Fiscal Year 2012: ¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000)
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| Keywords | 蛋白質工学 / 分子設計 / フォールディング / 酵素 |
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| Research Abstract |
Nowadays, artificially engineered proteins are utilized in various life science areas. In addition, engineered proteins are known to be one of promised candidates for next generation drugs. However, effective methods for rational design of artificial proteins have not been developed. Using comprehensive alanine insertion mutation analysis, we have revealed that regions of several subsequent amino acid residues in a protein, named as "Element", are responsible for the function and structure. In this work, we established a working hypothesis that those elements act as building blocks in the proteins, and verified it. In the result, by implanting the elements from a protein into another protein with a different function, we successfully modified the protein function as we wish. This success implies that the elements act as a kind of building-block to mediate a particular protein function, and will enable us to design an artificial protein rationally.
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Report
(4 results)
Research Products
(91 results)
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[Journal Article] Watching a signaling protein function in real time via 100-ps time-resolved Laue crystallography2012
Author(s)
Schotte F, Cho HS, Kaila VR, Kamikubo H, Dashdorj N, Henry ER, Graber TJ, Henning R, Wulff M, Hummer G, Kataoka M, Anfinrud PA
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Journal Title
Proc Natl Acad Sci USA
Volume: 109(47)
Pages: 19256-61
NAID
Related Report
Peer Reviewed
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