| Budget Amount *help |
¥19,760,000 (Direct Cost: ¥15,200,000、Indirect Cost: ¥4,560,000)
Fiscal Year 2013: ¥5,980,000 (Direct Cost: ¥4,600,000、Indirect Cost: ¥1,380,000)
Fiscal Year 2012: ¥6,630,000 (Direct Cost: ¥5,100,000、Indirect Cost: ¥1,530,000)
Fiscal Year 2011: ¥7,150,000 (Direct Cost: ¥5,500,000、Indirect Cost: ¥1,650,000)
|
|---|
| Research Abstract |
The question addressed here is whether dynein generates force by ATPase-dependent linker swing. The following system has been set up for observing the linker swing at a single molecule level. The motor domain was attached on glass surface through interactions between His-tags and anti-His-tag antibodies. A small bead was attached at the end of the linker through the avidin-biotin interaction. Beads motions were observed in the presence of ATP under a microscope. Some beads (~0.1%) showed ~90 degree rotation as expected for the ATPase-dependent linker swing. To check if these rotations correspond to linker swings, ATP was quickly exchanged to AMPPNP,which should stop the linker swing. Each rotating beads were checked immediately after the solution exchange. Rotation of each bead examined so far did not stop. Further data collection is required to pick up beads motions reflecting the linker swing.
|
