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Integrated understanding of regulations of DNA replication by cell cycle and chromatin

Research Project

Project/Area Number 23370076
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Research Field Molecular biology
Research InstitutionOsaka University

Principal Investigator

MASUKATA Hisao  大阪大学, 理学(系)研究科(研究院), 教授 (00199689)

Project Period (FY) 2011-04-01 – 2014-03-31
Project Status Completed (Fiscal Year 2013)
Budget Amount *help
¥20,020,000 (Direct Cost: ¥15,400,000、Indirect Cost: ¥4,620,000)
Fiscal Year 2013: ¥5,850,000 (Direct Cost: ¥4,500,000、Indirect Cost: ¥1,350,000)
Fiscal Year 2012: ¥6,760,000 (Direct Cost: ¥5,200,000、Indirect Cost: ¥1,560,000)
Fiscal Year 2011: ¥7,410,000 (Direct Cost: ¥5,700,000、Indirect Cost: ¥1,710,000)
KeywordsDNA複製 / 細胞周期 / クロマチン / テロメア / 分裂酵母 / シュゴシン / 複製制御 / 染色体 / 複製開始 / セントロメア / Taz1/TRF1 / Sgo2 / CDK / CMGヘリカーゼ / Mcm10 / replisome / 複製開始点 / DNAポリメラーゼイプシロン
Research Abstract

We investigated the molecular mechanisms how initiation of DNA replication is regulated by cell cycle and chromatin structures using model organism fission yeast. We found that phosphorylations of multiple replication factors, Sld3 and Drc1, by cell cycle master kinase CDK are crucial to trigger assembly of replication factors at replication origins. We further discovered the role of Mcm10 after assembly of replication factors to initiate actual DNA synthesis. In approach to elucidate the replication timing program, we discovered that telomere-repeat sequence is located near the late-replicating origins and the telomere-binding proteins are involved in replication timing program. These findings are published on high-quality international journals.

Report

(4 results)
  • 2013 Annual Research Report   Final Research Report ( PDF )
  • 2012 Annual Research Report
  • 2011 Annual Research Report
  • Research Products

    (35 results)

All 2013 2012 2011 Other

All Journal Article (11 results) (of which Peer Reviewed: 11 results) Presentation (17 results) (of which Invited: 5 results) Remarks (7 results)

  • [Journal Article] Fission Yeast RecQ Helicase Rqh1 Is Required for the Maintenance of Circular Chromosomes2013

    • Author(s)
      Tomoko Nanbu
    • Journal Title

      Mol. Cell. Biol.

      Volume: 33 Issue: 6 Pages: 1175-1187

    • DOI

      10.1128/mcb.01713-12

    • Related Report
      2013 Annual Research Report 2013 Final Research Report 2012 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Telomere-binding proteins play roles in control of replication timing2012

    • Author(s)
      Tazumi, A., Masukata, H.
    • Journal Title

      Cell Cycle

      Volume: 11(24) Issue: 18 Pages: 4492-4493

    • DOI

      10.1101/gad.194282.112

    • Related Report
      2013 Final Research Report 2012 Annual Research Report
    • Peer Reviewed
  • [Journal Article] DNA polymerization-independent functions of DNA polymerase epsilon in assembly and progression of the replisome in fission yeast2012

    • Author(s)
      Handa, T., Kanke, M., Takahashi, TS, Nakagawa, T., Masukata, H
    • Journal Title

      Mol. Biol. Cell

      Volume: 23(16) Pages: 3240-3253

    • Related Report
      2013 Final Research Report
    • Peer Reviewed
  • [Journal Article] The Prereplication Complex Recruits XEco2 to Chromatin to Promote Cohesin Acetylation in Xenopus Egg Extracts2012

    • Author(s)
      Torahiko L. Higashi
    • Journal Title

      Current Biology

      Volume: 22 Issue: 11 Pages: 977-988

    • DOI

      10.1016/j.cub.2012.04.013

    • Related Report
      2013 Final Research Report 2012 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Mcm10 plays an essential role in origin DNA unwinding after assembly of the CMG components2012

    • Author(s)
      Mai Kanke
    • Journal Title

      The EMBO Journal

      Volume: 31 Issue: 9 Pages: 2182-2194

    • DOI

      10.1038/emboj.2012.68

    • Related Report
      2013 Final Research Report 2012 Annual Research Report 2011 Annual Research Report
    • Peer Reviewed
  • [Journal Article] DNA polymerization-independent functions of DNA polymerase epsilon in assembly and progression of the replisome in fission yeast.2012

    • Author(s)
      Handa, T.
    • Journal Title

      Molecular Biology of the Cell

      Volume: 23 (16) Issue: 16 Pages: 3240-3253

    • DOI

      10.1091/mbc.e12-05-0339

    • Related Report
      2012 Annual Research Report
    • Peer Reviewed
  • [Journal Article] An abundance of pre-replicative complexes (pre-RCs) facilitates recombinational repair under replication stress in fission yeast2011

    • Author(s)
      Maki, K., Inoue, T., Onaka, A., Hashizume, H., Somete, N., Kobayashi, Y., Murakami, S., Shigaki, C., Takahashi, TS., Masukata, H., Nakagawa, T
    • Journal Title

      J. Biol. Chem.

      Volume: 286(48) Issue: 48 Pages: 41701-41710

    • DOI

      10.1074/jbc.m111.285619

    • Related Report
      2013 Final Research Report 2011 Annual Research Report
    • Peer Reviewed
  • [Journal Article] CDK promotes interactions of Sld3 and Drc1 for initiation of DNA replication in fission yeast2011

    • Author(s)
      Fukuura, M., Nagao, K., Obuse, C., Takahashi, TS., Nakagawa, T., Masukata, H
    • Journal Title

      Mol. Biol. Cell

      Volume: 22 Pages: 2620-2633

    • Related Report
      2013 Final Research Report
    • Peer Reviewed
  • [Journal Article] Auxin-inducible protein depletion system in fission yeast2011

    • Author(s)
      Kanke M
    • Journal Title

      BMC Cell Biology

      Volume: 12 Issue: 1 Pages: 8-8

    • DOI

      10.1186/1471-2121-12-8

    • Related Report
      2013 Final Research Report 2011 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Regulation of DNA replication by chromatin structures : accessibility and recruitment2011

    • Author(s)
      Hayashi, MT., Masukata, H
    • Journal Title

      Chromosoma

      Volume: 120 Pages: 39-46

    • Related Report
      2013 Final Research Report
    • Peer Reviewed
  • [Journal Article] CDK promotes interactions of Sld3 and Drc1 with Cut5 for initiation of DNA replication in fission yeast2011

    • Author(s)
      Fukuura M.、 Nagao K.、 Obuse C.、 Takahashi T.S.、 Nakagawa T.、Masukata H.
    • Journal Title

      Molecular biology of the cell

      Volume: 22(14) Issue: 14 Pages: 2620-2633

    • DOI

      10.1091/mbc.e10-12-0995

    • Related Report
      2011 Annual Research Report
    • Peer Reviewed
  • [Presentation] Replication timing control by telomere proteins2013

    • Author(s)
      升方久夫
    • Organizer
      第21回国際遺伝学会・Human Genome Meeting 2013
    • Place of Presentation
      Singapore
    • Related Report
      2013 Annual Research Report 2013 Final Research Report
  • [Presentation] Replication timing program in fission yeast2013

    • Author(s)
      升方久夫
    • Organizer
      第7回分裂酵母国際学会
    • Place of Presentation
      London UK
    • Related Report
      2013 Final Research Report
  • [Presentation] Replication timing control by centromere and telomere binding proteins2013

    • Author(s)
      升方久夫
    • Organizer
      酵母エピジェネティックミーティング
    • Place of Presentation
      福井県あわら市
    • Related Report
      2013 Annual Research Report 2013 Final Research Report
  • [Presentation] Replication timing control by chromatin proteins2013

    • Author(s)
      升方久夫
    • Organizer
      Eukaryotic DNA replication and genome maintenance
    • Place of Presentation
      Cold Spring Harbor, NY, USA
    • Related Report
      2013 Annual Research Report 2013 Final Research Report
  • [Presentation] Regulation of replication program in fission yeast : replicator, initiator and regulators2013

    • Author(s)
      升方久夫
    • Organizer
      第36回日本分子生物学会年会
    • Place of Presentation
      神戸
    • Related Report
      2013 Final Research Report
  • [Presentation] Replication timing program in fission yeast2013

    • Author(s)
      升方久夫
    • Organizer
      第7回分裂酵母国際学会
    • Place of Presentation
      London, UK
    • Related Report
      2013 Annual Research Report
    • Invited
  • [Presentation] Regulation of replication program in fission yeast: replicator, initiator and regulators2013

    • Author(s)
      升方久夫
    • Organizer
      第36回日本分子生物学会年会
    • Place of Presentation
      神戸
    • Related Report
      2013 Annual Research Report
    • Invited
  • [Presentation] Activation of replication origins and involvement of telomere proteins in timing control2012

    • Author(s)
      升方久夫
    • Organizer
      DNA replication of eukaryotic genomes at the Foundation des Treilles
    • Place of Presentation
      Les Treilles, Les Arcs, France
    • Related Report
      2013 Final Research Report
  • [Presentation] Control of global replication timing by telomere-binding proteins in fission yeast2012

    • Author(s)
      升方久夫
    • Organizer
      第8回3Rシンポジウム
    • Place of Presentation
      淡路夢舞台
    • Related Report
      2013 Final Research Report
  • [Presentation] Replication Timing Program in Fission Yeast2012

    • Author(s)
      升方久夫
    • Organizer
      第35回日本分子生物学会年会
    • Place of Presentation
      福岡
    • Related Report
      2013 Final Research Report
  • [Presentation] Activation of replication origins and involvement of telomere proteins in timing control.2012

    • Author(s)
      升方久夫
    • Organizer
      DNA replication of eukaryotic genomes at the Foundation des Treilles.
    • Place of Presentation
      Les Treilles, Les Arcs, France
    • Related Report
      2012 Annual Research Report
    • Invited
  • [Presentation] Control of global replication timing by telomere-binding proteins in fission yeast2012

    • Author(s)
      升方久夫
    • Organizer
      The 8th 3R Symposium
    • Place of Presentation
      Yumebutai, Awaji, Japan
    • Related Report
      2012 Annual Research Report
    • Invited
  • [Presentation] Replication Timing Program in Fission Yeas2012

    • Author(s)
      升方久夫
    • Organizer
      第34回日本分子生物学会年会
    • Place of Presentation
      福岡国際会議場、福岡
    • Related Report
      2012 Annual Research Report
    • Invited
  • [Presentation] Telomere-binding protein Taz1 controls replication timing of the internal late origins in fission yeast2011

    • Author(s)
      升方久夫
    • Organizer
      第34回日本分子生物学会年会
    • Place of Presentation
      パシフィコ横浜(神奈川県)
    • Year and Date
      2011-12-15
    • Related Report
      2013 Final Research Report 2011 Annual Research Report
  • [Presentation] Replication timing control by Taz1 that binds to telomeric repeats proximal to internal late replication origins2011

    • Author(s)
      升方久夫
    • Organizer
      Eukaryotic DNA Replication and Genome Maintenance
    • Place of Presentation
      Cold Spring Harbor, NY, USA
    • Year and Date
      2011-09-07
    • Related Report
      2013 Final Research Report
  • [Presentation] Replication timing control by Taz1 that binds to telomeric repeats proximal to internal late replication origins2011

    • Author(s)
      升方久夫
    • Organizer
      Eukaryotic DNA Replication and Genome Maintenance
    • Place of Presentation
      Cold Spring Harbor, USA
    • Year and Date
      2011-09-07
    • Related Report
      2011 Annual Research Report
  • [Presentation] Regulation of replication of fission yeast genome2011

    • Author(s)
      升方久夫
    • Organizer
      The Sixth International Fission Yeast Meeting
    • Place of Presentation
      Boston, USA
    • Year and Date
      2011-06-27
    • Related Report
      2013 Final Research Report 2011 Annual Research Report
  • [Remarks]

    • URL

      http://www.bio.sci.osaka-u.ac.jp/dbs01/re-paper-temp.php?id=14

    • Related Report
      2013 Final Research Report
  • [Remarks]

    • URL

      http://www.bio.sci.osaka-u.ac.jp/bio_web/lab_page/masukata/thesis/index.html

    • Related Report
      2013 Final Research Report
  • [Remarks] 大阪大学理学研究科生物科学専攻分子遺伝学研究室

    • URL

      http://www.bio.sci.osaka-u.ac.jp/dbs01/re-paper-temp.php?id=14

    • Related Report
      2013 Annual Research Report
  • [Remarks] 升方研究室

    • URL

      http://www.bio.sci.osaka-u.ac.jp/bio_web/lab_page/masukata/thesis/index.html

    • Related Report
      2013 Annual Research Report
  • [Remarks] 大阪大学理学研究科生物科学専攻升方研究室

    • URL

      http://www.bio.sci.osaka-u.ac.jp/bio_web/lab_page/masukata/

    • Related Report
      2012 Annual Research Report
  • [Remarks] 大阪大学理学研究科生物科学専攻研究室紹介升方研究室

    • URL

      http://www.bio.sci.osaka-u.ac.jp/dbs01/re-paper-temp.php?id=14

    • Related Report
      2012 Annual Research Report
  • [Remarks]

    • URL

      http://www.bio.sci.osaka-u.ac.jp/bio_web/lab_page/masukata/index.html

    • Related Report
      2011 Annual Research Report

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Published: 2011-04-06   Modified: 2019-07-29  

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