Integrated understanding of regulations of DNA replication by cell cycle and chromatin
| Project/Area Number |
23370076
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Molecular biology
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| Research Institution | Osaka University |
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Principal Investigator |
MASUKATA Hisao 大阪大学, 理学(系)研究科(研究院), 教授 (00199689)
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| Project Period (FY) |
2011-04-01 – 2014-03-31
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| Project Status |
Completed (Fiscal Year 2013)
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| Budget Amount *help |
¥20,020,000 (Direct Cost: ¥15,400,000、Indirect Cost: ¥4,620,000)
Fiscal Year 2013: ¥5,850,000 (Direct Cost: ¥4,500,000、Indirect Cost: ¥1,350,000)
Fiscal Year 2012: ¥6,760,000 (Direct Cost: ¥5,200,000、Indirect Cost: ¥1,560,000)
Fiscal Year 2011: ¥7,410,000 (Direct Cost: ¥5,700,000、Indirect Cost: ¥1,710,000)
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| Keywords | DNA複製 / 細胞周期 / クロマチン / テロメア / 分裂酵母 / シュゴシン / 複製制御 / 染色体 / 複製開始 / セントロメア / Taz1/TRF1 / Sgo2 / CDK / CMGヘリカーゼ / Mcm10 / replisome / 複製開始点 / DNAポリメラーゼイプシロン |
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| Research Abstract |
We investigated the molecular mechanisms how initiation of DNA replication is regulated by cell cycle and chromatin structures using model organism fission yeast. We found that phosphorylations of multiple replication factors, Sld3 and Drc1, by cell cycle master kinase CDK are crucial to trigger assembly of replication factors at replication origins. We further discovered the role of Mcm10 after assembly of replication factors to initiate actual DNA synthesis. In approach to elucidate the replication timing program, we discovered that telomere-repeat sequence is located near the late-replicating origins and the telomere-binding proteins are involved in replication timing program. These findings are published on high-quality international journals.
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Report
(4 results)
Research Products
(35 results)
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[Journal Article] An abundance of pre-replicative complexes (pre-RCs) facilitates recombinational repair under replication stress in fission yeast2011
Author(s)
Maki, K., Inoue, T., Onaka, A., Hashizume, H., Somete, N., Kobayashi, Y., Murakami, S., Shigaki, C., Takahashi, TS., Masukata, H., Nakagawa, T
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Journal Title
J. Biol. Chem.
Volume: 286(48)
Issue: 48
Pages: 41701-41710
DOI
Related Report
Peer Reviewed
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[Journal Article] CDK promotes interactions of Sld3 and Drc1 for initiation of DNA replication in fission yeast2011
Author(s)
Fukuura, M., Nagao, K., Obuse, C., Takahashi, TS., Nakagawa, T., Masukata, H
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Journal Title
Mol. Biol. Cell
Volume: 22
Pages: 2620-2633
Related Report
Peer Reviewed
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