Dynamics of human genome DNA in living cells revealed by nucleosome imaging
| Project/Area Number |
23370078
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Molecular biology
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| Research Institution | National Institute of Genetics |
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Principal Investigator |
MAESHIMA Kazuhiro 国立遺伝学研究所, 構造遺伝学研究センター, 教授 (00392118)
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| Project Period (FY) |
2011-04-01 – 2015-03-31
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| Project Status |
Completed (Fiscal Year 2014)
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| Budget Amount *help |
¥20,150,000 (Direct Cost: ¥15,500,000、Indirect Cost: ¥4,650,000)
Fiscal Year 2013: ¥2,990,000 (Direct Cost: ¥2,300,000、Indirect Cost: ¥690,000)
Fiscal Year 2012: ¥8,060,000 (Direct Cost: ¥6,200,000、Indirect Cost: ¥1,860,000)
Fiscal Year 2011: ¥9,100,000 (Direct Cost: ¥7,000,000、Indirect Cost: ¥2,100,000)
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| Keywords | ゲノム / 遺伝学 / 核酸 / 生物物理 |
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| Outline of Final Research Achievements |
Genome information, which is three-dimensionally organized within cells as chromatin, is searched and read out by various proteins for diverse cell functions. Although how the protein factors find their targets remains unclear, the dynamic nature of chromatin is likely crucial. To pursue this dynamics, we investigated the movement of each nucleosome in the chromatin. It had been technically challenging to image single nucleosome, because a single cell has numerous nucleosomes (≈3 x 107). To fluorescently label a small number of nucleosomes, we fused PA-GFP (PhotoActivatable-GFP) to histone H4, and stably expressed the PA-GFP-H4 in the cells. From our analysis of the single nucleosome movement, we found a local fluctuation of individual nucleosomes. This local dynamic movement would be a basis of various genome events.
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Report
(4 results)
Research Products
(38 results)
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[Journal Article] Lamin B2 prevents chromosome instability by ensuring proper mitotic chromosome segregation2013
Author(s)
Kuga, T., Nie, H., Kazami, T., Satoh, M., Matsushita, K., Nomura, F., Maeshima, K., Nakayama, Y., Tomonaga, T.
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Journal Title
Oncogenesis
Volume: 3
Issue: 3
Pages: e94-e94
DOI
Related Report
Peer Reviewed
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[Journal Article] Chromatin compaction protects genomic DNA from radiation damage.2013
Author(s)
Takata, H., Hanafusa, T., Mori, T., Shimura, M., Iida,Y., Ishikawa, K., Yoshikawa, K., Yoshikawa, Y., Maeshima, K.
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Journal Title
PLOS ONE
Volume: 8
Issue: 10
Pages: e75622-e75622
DOI
Related Report
Peer Reviewed / Open Access
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[Journal Article] Chromosome engineering allows the efficient isolation of vertebrate neocentromeres2013
Author(s)
Shang WH, Hori T, Martins NM, Toyoda A, Misu S, Monma N, Hiratani I, Maeshima K, Ikeo K, Fujiyama A, Kimura H, Earnshaw WC, and Fukagawa T
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Journal Title
Dev Cell
Volume: 24
Issue: 6
Pages: 635-648
DOI
Related Report
Peer Reviewed
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