| Budget Amount *help |
¥20,150,000 (Direct Cost: ¥15,500,000、Indirect Cost: ¥4,650,000)
Fiscal Year 2013: ¥2,990,000 (Direct Cost: ¥2,300,000、Indirect Cost: ¥690,000)
Fiscal Year 2012: ¥8,060,000 (Direct Cost: ¥6,200,000、Indirect Cost: ¥1,860,000)
Fiscal Year 2011: ¥9,100,000 (Direct Cost: ¥7,000,000、Indirect Cost: ¥2,100,000)
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| Outline of Final Research Achievements |
Genome information, which is three-dimensionally organized within cells as chromatin, is searched and read out by various proteins for diverse cell functions. Although how the protein factors find their targets remains unclear, the dynamic nature of chromatin is likely crucial. To pursue this dynamics, we investigated the movement of each nucleosome in the chromatin. It had been technically challenging to image single nucleosome, because a single cell has numerous nucleosomes (≈3 x 107). To fluorescently label a small number of nucleosomes, we fused PA-GFP (PhotoActivatable-GFP) to histone H4, and stably expressed the PA-GFP-H4 in the cells. From our analysis of the single nucleosome movement, we found a local fluctuation of individual nucleosomes. This local dynamic movement would be a basis of various genome events.
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